EFFICIENT  in  vitro  PLANT  REGENERATION  FROM  CULTURED LEAF  AND  PETIOLE  EXPLANTS  OF  Isatis  constricta  Davis

Özel, Çiğdem Alev

doi:10.24326/asphc.2018.3.5

Cited by 3 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BA plays an essential role in building RNA, which leads to the building of amino acids and proteins. Thus increasing the biomass of the plant cell [16,17,18]. Table 7.…”

Section: 7mentioning

confidence: 99%

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

Rashid,

Neamah

2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Obtaining callus tissue is one of the essential aims of plant tissue culture technology due to its great importance in subsequent experiments. The present study aimed to determine the interactive effects of plant growth regulators in stimulating calli cultures from the hypocotyl of Phaseolus vulgaris. The study was conducted in the Plant Tissue Culture Laboratory, Desert Studies Center, University of Anbar. Different concentrations of 2,4-D were used, including 1.0, 2.0, 3.0, and 4.0 mg L−1, with concentrations of BA including 0, 0.5, 1.0, 1.5, and 2.0 mg L−1. Sodium hypochlorite (NaOCl) was used to obtain sterile seedlings. The seeds were sterilized with four concentrations of 0, 2, 4 and 6% for 5, 10 and 15 min. We use the complete random design (RCD) for coefficient distribution. The results showed the mixture of NaOCl 4.0% for 10 min recorded the highest percentage of sterile seedling production (SSP) at 90%. As for the induction of callus culture, the hypocotyl extracted from sterilized seedlings was grown in MS medium supplemented with 2,4-D and BA concentrations for 21 days. The results showed that the combination 2.0+1.5 mg L−1 caused the highest callus formation response (CFR) by 100%, the lowest complete callus formation (CCF) by 5.80 days, and the highest fresh weight (FW) by 4.318 g. At the same time, the combination of 2.0+2.0 mg L−1 reduced the start of callus formation (SCF) by 3.40 days.

show abstract

“…BA plays an essential role in building RNA, which leads to the building of amino acids and proteins. Thus increasing the biomass of the plant cell [16,17,18]. Table 7.…”

Section: 7mentioning

confidence: 99%

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

Rashid,

Neamah

2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…It is an important rootstock for a number of citrus fruit crops including oranges, grape fruits, Kinnows, lemons, and mandarins all over the world (Kaur, 2018) [9] . In vitro culture is a method can produce crops on a comparatively large scale in comparison with conventional plant breeding and also results in superior soma clonal variants (Kasprzyk-Pawelec et al, 2015) [7] . The development of the in vitro regeneration protocol is essential to be used routinely as a research tool for the improvement of this plant species.…”

Section: Introductionmentioning

confidence: 99%

Effect of different growth regulators on in vitro regeneration of cotyledon explants of rough lemon (Citrus jambhiri Lush.) via callus induction

Iqbal¹,

Bakshi²,

Kour³

et al. 2021

Int. J. Chem. Stud.

View full text Add to dashboard Cite

In order to establish in vitro protocols for raising aseptic cultures of cotyledon segments excised from five-week-old in vitro raised seedlings were used as explant material. Among surface sterilants, mercuric chloride 0.1% for three minutes provided least rate of contamination (24%) and the maximum explant survival (76%). The highest per cent culture establishment of 97.79% was obtained in cotyledon explants on Murashige and Skoog (MS) containing 2,4-D (3.0 mg/l) and maximum callus induction (95.41%) was observed on MS medium supplemented with 2,4-D (2.0 mg/l). Among different plant growth regulator treatments, maximum callus regeneration of 82.19% was observed on MS medium supplemented with BAP (3.0 mg/l) whereas, minimum callus regeneration (4.33%) was found in MS medium supplemented with BAP (3.0 mg/l) in combination with IAA (0.75 mg/l) and malt extract (500 mg/l). Maximum rooting response (96.00%) was recorded on MS (half strength) medium with NAA (2.0 mg/l) and was at par with rooting percentage of 92.00% on MS (half strength) medium supplemented with NAA (1.5 mg/l). The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (91.35%) was achieved in potting mixture containing garden soil with sand and farmyard manure (1:1:1).

show abstract

Sucul Bitki Bacopa monnieri (L.) Wettst.’nin Yaprak Eksplantlarından Çoklu Sürgün Rejenerasyonu Üzerine Zeatin Ön Uygulamasının Etkisi

Doğan

2019

Journal of Anatolian Environmental and Animal Sciences

View full text Add to dashboard Cite

Öz: Bacopa monnieri (L.) Wettst su ortamının iyileştirilmesinde ve geleneksel tıp sisteminde önemli bir bitkidir. Bu çalışmada, B. monnieri'nin doku kültürü teknikleri ile üretimi hedeflenmiştir. B. monneri'nin yaprak eksplantları farklı dozlarda Zeatin (ZEA)'li solüsyonlarda (0.5-8.0 mg/L) bir saat bekletilmiş ve ardından bitki büyüme düzenleyici içermeyen kültür ortamına aktarılmıştır. Yaprak eksplantlarından ilk sürgün çıkışları 16. günde 4 mg/L ZEA ön uygulamasında gözlenmiştir. Sürgün rejenerasyon frekansı % 16.66-88.89 arasında sıralanmıştır. En yüksek sürgün rejenerasyon frekansları 4 ve 8 mg/L ZEA ön uygulamasında elde edilmiştir. Genel olarak ZEA konsantrasyonu arttıkça sürgün sayısı ve sürgün uzunluğu da artmıştır. Eksplant başına maksimum sürgün sayısı (10.31 sürgün/eksplant) 4 mg/L ZEA ön uygulamasında, en uzun sürgünler ise (2.02 cm) 8 mg/L ZEA ön uygulamasında tespit edilmiştir. Rejenere sürgünler 0.25 mg/L indol-3-butirik asit (IBA) içeren kültür ortamında köklendirildikten sonra dış koşullara başarıyla alıştırılmıştır. Sonuç olarak, ZEA ön uygulamasının B. monnieri'nin in vitro üretimindeki etkinliği kaydedilmiştir.

show abstract

EFFICIENT in vitro PLANT REGENERATION FROM CULTURED LEAF AND PETIOLE EXPLANTS OF Isatis constricta Davis

Cited by 3 publications

References 8 publications

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

2,4-Dichlorophenoxyacetic Acid and 6-Benzyladenine Stimulate of Indication and Biomass Accumulation in a Phaseolus Vulgaris Callus Culture

Effect of different growth regulators on in vitro regeneration of cotyledon explants of rough lemon (Citrus jambhiri Lush.) via callus induction

Sucul Bitki Bacopa monnieri (L.) Wettst.’nin Yaprak Eksplantlarından Çoklu Sürgün Rejenerasyonu Üzerine Zeatin Ön Uygulamasının Etkisi

Contact Info

Product

Resources

About