2022
DOI: 10.3390/plants11151937
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Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Abstract: Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and t… Show more

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Cited by 15 publications
(3 citation statements)
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“…The initial requirement for establishing a plant in vitro fast propagation system is gaining active and sterile ingredients (Gu et al 2022). Explants from lacquer trees secrete raw lacquer and form an oxidized layer at the incision, which prevents them from absorbing nutrients and plant development regulators from the media (Kitajima et al 2017).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The initial requirement for establishing a plant in vitro fast propagation system is gaining active and sterile ingredients (Gu et al 2022). Explants from lacquer trees secrete raw lacquer and form an oxidized layer at the incision, which prevents them from absorbing nutrients and plant development regulators from the media (Kitajima et al 2017).…”
Section: Discussionmentioning
confidence: 99%
“…However, the lacquer sap produced by triploid lacquer trees is easily oxidized in the air to form a black lm, which obstructs the outgrowth's ability to absorb nutrients and, in extreme situations, can result in the material's death. A huge number of endophytic bacteria are present in the external environment, which is persistently warm and rainy, making it challenging for surface disinfection techniques to e ciently eradicate them (Li et al 2022). Consequently, the propagation of triploid lacquer trees in vitro has become challenging, hindering the development of effective seedling breeding techniques.…”
Section: Introductionmentioning
confidence: 99%
“…For example, mercury chloride was used successfully for Commiphora gileadensis L. leaf sterilization [30] and also for stem segment sterilization in Cnidoscolus aconitifolius (Mill.) [31]. About 95% of the cultured S. dominica L. axillary buds were developed successfully on MS media supplemented with 0.5 mg L −1 of GA 3 after four weeks of establishment.…”
Section: Plant Sterilization and In Vitro Establishmentmentioning
confidence: 96%