Efficient isolation of the rare diarrhoeic shellfish toxin, dinophysistoxin-2, from marine phytoplankton

James, Kevin J.; Bishop, Alan; Healy, Brendan M; Roden, Cilian; Sherlock, Ian R.; Twohig, Marian; Draisci, Rosa; Giannetti, Luigi; Lucentini, Luca

doi:10.1016/s0041-0101(98)00184-6

Cited by 34 publications

(13 citation statements)

References 29 publications

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“…DTX1 was first detected in Dinophysis fortii in Japan; DTX2 was identified in shellfish in Ireland during a DSP episode (Van Egmond et al, 1993). DTX2 was isolated also from a marine phytoplankton biomass mainly consisting of Dinophysis acuta (James et al, 1999). A new isomer of DTX2, named DTX2B, was isolated and identified in Irish mussel extracts (James et al, 1997).…”

Section: Chemical Propertiesmentioning

confidence: 99%

Marine biotoxins and its detection

Suresh¹,

Gauri²,

Desai³

et al. 2014

Afr. J. Environ. Sci. Technol.

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The incidences of intoxication due to the consumption of marine foods have been increasing in recent years. This is due to the presence of biotoxins in foods of marine origin. The biotoxins will be accumulated in the marine foods due to the consumption of toxic biota of marine origin. When this contaminated food is taken by the humans or animals, those toxins will be transferred to them causing intoxication and lethality. Among these intoxications, most of them are caused by the harmful algal blooms (HAB). In order to avoid the harmful effects from marine biotoxins, it is necessary to have the proper knowledge. In this manuscript, the different types of biotoxins, source of intoxication, characteristics of toxins, detection and control measures are discussed in detail.

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Section: Chemical Propertiesmentioning

confidence: 99%

Marine biotoxins and its detection

Suresh¹,

Gauri²,

Desai³

et al. 2014

Afr. J. Environ. Sci. Technol.

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“…Further, profile and toxin content per cell are needed to parameterize models on uptake and detoxification kinetics by different shellfish species (Blanco et al, 1995) because different toxins-okadaic acid (OA), dinophysistoxin-2 (DTX2)-exhibit important differences in their detoxification kinetics (Blanco et al, 2005). Studies on the toxin profile and content of D. acuta by high performance liquid chromatography (HPLC) in both plankton concentrates rich in this species and in single cell isolates (''picked cells'') showed that D. acuta from Ireland, Spain, and Portugal contained OA and DTX2 (James et al, 1999;Vale and Sampayo, 2000;Ferná ndez et al, 2001). More recent studies by liquid chromatography coupled to mass spectrometry (LC-MS) have shown a widespread presence of pectenotoxins (PTXs) in all D. acuta populations tested from New Zealand and Europe , and also the identification of OAdiol-esters in strains from New Zealand (Suzuki et al, 2004) and Spain (Pizarro et al, 2008a).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variability of lipophilic toxins during a Dinophysis acuta bloom in Western Iberia: Differences between picked cells and plankton concentrates

et al. 2009

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“…Dinophysistoxin-2 (DTX-2) was isolated from contaminated mussels [17] and 7-epipectenotoxin-2 seco acid (7-epi-PTX2SA) was isolated from phytoplankton biomass that was predominantly D. acuta [23]. A certified standard lyophilised mussel material (MUS-2, National Research Council, Halifax, Canada) containing two DSP toxins was reconstituted in methanol immediately prior to use to give a mixture with 2.5 µg total toxins mL -1 (2.29 µg OA, certified, and 0.21 µg DTX-1 mL -1 , uncertified).…”

Section: Materials and Chemicalsmentioning

confidence: 99%

“…4-bromomethyl-7-methoxycoumarin, BrMMC) [15,16], quinoxalinone derivatives (e.g. 3-bromomethyl-6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone, BrDMEQ) [17], luminarines [18], 9-chloromethylanthracene (CA) [19], N-(9-acridinyl)bromoacetamide (NABA) [20], 1-bromoacetylpyrene (BAP) [21,22], and 4-[2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalyl-ethyl]-1,2,4-triazoline-3,5-dione (DMEQ-TAD) [23].…”

Section: Introductionmentioning

confidence: 99%

Comparison of different fluorimetric HPLC methods for analysis of acidic polyether toxins in marine phytoplankton

Nogueiras

Gago-Martı́nez

Paniello

et al. 2003

Analytical and Bioanalytical Chemistry

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The human toxic syndrome, diarrhetic shellfish poisoning (DSP), is caused by polyether toxins that are present in bivalve molluscs but originate from some species of marine phytoplankton. During the last few years different HPLC methods with fluorescence detection (FLD) have been proposed for analysis of marine toxins, including polyether toxins, in shellfish and phytoplankton. Several derivatization reagents have been proposed in the literature, with the aim of converting the acidic DSP toxins into their corresponding fluorescent derivatives. In this work we report results obtained from HPLC-FLD analysis of extracts from phytoplankton, including Dinophysis spp.,harvested off the south-west coast of Ireland. Three different reagents were used for fluorescent derivatization: 3-bromomethyl-6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone (BrDMEQ), 9-chloromethylanthracene (CA), and "in situ" 9-anthracenyldiazomethane (ADAM). Derivatization was performed under conditions previously optimised. The DSP derivatives were cleaned using different SPE procedures then analysed by HPLC-FLD. In this study, the use of BrDMEQ, CA, and "in situ" ADAM was compared in terms of sensitivity and selectivity. Evaluation of HPLC methods for analysis of DSP toxin derivatives was also conducted; the presence of okadaic acid (OA), dinophysistoxin-2 (DTX-2), and pectenotoxin-2 seco acids (PTX1SAs) was detected in the sample extracts studied.

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Efficient isolation of the rare diarrhoeic shellfish toxin, dinophysistoxin-2, from marine phytoplankton

Cited by 34 publications

References 29 publications

Marine biotoxins and its detection

Marine biotoxins and its detection

Seasonal variability of lipophilic toxins during a Dinophysis acuta bloom in Western Iberia: Differences between picked cells and plankton concentrates

Comparison of different fluorimetric HPLC methods for analysis of acidic polyether toxins in marine phytoplankton

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