Efficient isolation of thermophilic and thermotolerant mucoralean fungi

Tansey, Michael R.

doi:10.1007/bf00436699

Cited by 12 publications

(6 citation statements)

References 20 publications

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“…It became known as a taxonomically selective fungicide when Bollen and Fuchs (3) and Edgington et al (6) demonstrated that it inhibited most ascomycetous fungi (including biologically related anamorphic fungi also classifiable in the anamorph division Fungi Imperfecti) but caused slight or no inhibition for most basidiomycetous and zygomycetous fungi. Since then it has been used in numerous selective media for the division Basidiomycota (8), the division Zygomycota (17), and members of the resistant genus Alternaria (1). It has also been used to aid in the classification of fungi of uncertain taxonomic disposition, particularly nonsporulating entities such as the E-strain somatic anamorphs of the discomycetous Tricharina species (4).…”

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confidence: 99%

The benomyl test as a fundamental diagnostic method for medical mycology

Summerbell

1993

J Clin Microbiol

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The fungicide benomyl has long been known to differentially affect major taxonomic groups of fungi. In the present study 163 species or aggregates of closely similar species of medically important fungi and actinomycetes, as well as species commonly isolated as clinical contaminants, were tested to determine their reactions to three concentrations of benomyl. Fungi of basidiomycetous, endomycetous, and microascaceous affinities were highly resistant, including all common yeasts and Geotrichum, Pseudallescheria, Scedosporium, and Scopulariopsis species. Also resistant were fungi of pleosporalean affinities with poroconidial anamorphs, such as Alternaria, Bipolaris, Curvularia, and Exserohilum species. Most other fungi of ascomycetous affinity were moderately to strongly susceptible. Such fungi included dermatophytes; Coccidioides, Blastomyces, and Histoplasma species; Sporothrix schenckii; medically important aspergilli; and "black yeasts." Benomyl testing aided in the provisional identification of nonsporulating mycelia, including common basidiomycetous isolates obtained as contaminants as well as nonsporulating Aspergillus fumigatus from pulmonary sources.

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confidence: 99%

The benomyl test as a fundamental diagnostic method for medical mycology

Summerbell

1993

J Clin Microbiol

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“…In addition, the purity of the isolated fungus was confirmed by microscopic examination of the culture at 400X magnification using light microscopy. After ensuring purity, the cultures were subcultured on PDA slants and allowed to grow for a period of 3-5 days and subsequently stored at 4°C as stock cultures [29].…”

Section: Direct Soil Plating By Warcup Methodsmentioning

confidence: 99%

Screening and isolation of substitute-rennet producing thermophilic phycomycetes, by modified Warcup method and improved selective medium

Khademi¹,

Abachi²,

Mortazavi³

2013

IOSR-JAVS

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Fungal milk-clotting enzymes are as valuable as bovine Chymosin in the cheese industry. Members of the Mucoraceae family, have important biotechnological potential as some species are enzyme producers at industrial scale. In this study, 31 isolates of thermophilic Phycomycetes of different taxa were selected from different sample sites and were studied for their aspartic protease producing capabilities. For primary isolation, modified Warcup method using improved Phycomycetes selective solid media along with high temperature selective for thermophilic Mucorals were applied. The purpose of this study was to collect the local biotechnologically important strains of thermophilic phycomycetes capable of producing rennin-like proteases. Based on a high relative enzyme activity (REA), the majority (83.7%) of the 31 species of isolated thermophilic Phycomycetes were selected for next isolation steps. By trial production of acid proteases using shake flask fermentation, Milk clotting activity (MCA), and Protease activity (PA) of each fermentation broth of primary isolates were measured. Later, based on their high MCA/PA ratio, three isolates were chosen for macroscopic and microscopic examinations of each isolate determining their taxonomy identification and classification. The results showed that two of final isolates (HPA-01 and ZDO-16) belong to Rhizomucor family (R. nainitalensis and R. pusillus) and the third thermophilic Zygomycetes (ZDP-03) is a Rhizopus (R. rhizopodiformis). Of the 3 isolates, Rhizomucor nainitalensis was chosen for further studies and could be introduced as a commercially important isolate in future.

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“…However, Malbranchea sulfurea and Talaroniyces cluponti did not show any proteolytic activity. TANSEY (1984) isolated several thermophilic fungi such as A.fumigutus, R . pusillus, T. lunuginosus, Chuetomium thermophilum, Rhizopus rhizopodiforrnis and Paecilomyces vuriotii from different sources including bran, corn meal, rice seeds, sunflower seeds and others.…”

Section: Tsiklinskymentioning

confidence: 99%

Some physiological studies on fungi isolated from poultry feedstuffs

Megalla

Nassar

Moharram

et al. 1990

J. Basic Microbiol.

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A total of 506 isolates of mesophilic, thermophilic and thermotolerant fungi isolated from the poultry feed ingredients included soybean meals, ground maize, cottonseed cake, wheat bran and fish meal, on glucose-Czapek's agar, Littman oxgall agar at 28 degrees C and yeast starch agar (YPSs) at 45 degrees C, were screened for their ability to produce hydrolytic protease enzyme on solid media. Most of the fungal isolates were able to produce such enzymes but with variable capabilities. The highest proteolytic activity was exhibited by some isolates of Penicillium chrysogenum, Aspergillus flavus, Thermoascus thermophilus and Rhizopus chizopodifarmis. Of all fungal isolates screened for proteolytic activity, Penicillium chrysogenum and Thermoascus thermophilus produced the highest amounts of proteases. These two isolates were used to study the effect of some environmental and nutritional factors on their proteolytic activity. It was found that the highest yield of protease by P. chrysogenum (12.5 units) was achieved 3 days after incubation at 30 degrees C. Marked reduction in protease activity was observed at 37 degrees C. The thermophilic fungus T. thermophillus exhibited maximum (18 units) proteolytic activity 6 days after incubation at 45 degrees C. The enzyme yield was reduced to 13 units at 50 degrees C. Among the seven carbon sources tested, sucrose was the most appropriate for maximum protease production by both P. chrysogenum and T. thermophilus (13.2 and 12.8 units, respectively). Of the sixteen nitrogen sources investigated, NaNO3 was the best inorganic additive nitrogenous salt which induced the highest proteolytic activity by P. chrysogenum and T. thermophilus, whereas DL-tryptophan was the most preferable organic nitrogen compound for maximum protease production by the two fungi tested.

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Efficient isolation of thermophilic and thermotolerant mucoralean fungi

Cited by 12 publications

References 20 publications

The benomyl test as a fundamental diagnostic method for medical mycology

The benomyl test as a fundamental diagnostic method for medical mycology

Screening and isolation of substitute-rennet producing thermophilic phycomycetes, by modified Warcup method and improved selective medium

Some physiological studies on fungi isolated from poultry feedstuffs

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