Efficient label-free in vivo photoacoustic imaging of melanoma cells using a condensed NIR-I spectral window

Cho, Soon-Woo; Phan, Thi Tuong Vy; Nguyen, Van Tu; Park, Sang Min; Lee, Hwidon; Oh, Junghwan; Kim, Chang-Seok

doi:10.1016/j.pacs.2023.100456

Cited by 11 publications

(6 citation statements)

References 86 publications

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“…Our primary source was Google Scholar, which yielded 12,300 total results. Of the 12,300 results, 102 studies 103,109,113,[115][116][117][132][133][134][135][136][137][138][139][140] met our criteria and are reviewed here. 3 Investigations of CM Using PAI…”

Section: Methodsmentioning

confidence: 99%

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Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Fakhoury,

Lara,

Manwar

et al. 2024

J. Biomed. Opt.

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Significance: Cutaneous melanoma (CM) has a high morbidity and mortality rate, but it can be cured if the primary lesion is detected and treated at an early stage. Imaging techniques such as photoacoustic (PA) imaging (PAI) have been studied and implemented to aid in the detection and diagnosis of CM.Aim: Provide an overview of different PAI systems and applications for the study of CM, including the determination of tumor depth/thickness, cancer-related angiogenesis, metastases to lymph nodes, circulating tumor cells (CTCs), virtual histology, and studies using exogenous contrast agents.Approach: A systematic review and classification of different PAI configurations was conducted based on their specific applications for melanoma detection. This review encompasses animal and preclinical studies, offering insights into the future potential of PAI in melanoma diagnosis in the clinic.Results: PAI holds great clinical potential as a noninvasive technique for melanoma detection and disease management. PA microscopy has predominantly been used to image and study angiogenesis surrounding tumors and provide information on tumor characteristics. Additionally, PA tomography, with its increased penetration depth, has demonstrated its ability to assess melanoma thickness. Both modalities have shown promise in detecting metastases to lymph nodes and CTCs, and an alloptical implementation has been developed to perform virtual histology analyses. Animal and human studies have successfully shown the capability of PAI to detect, visualize, classify, and stage CM.Conclusions: PAI is a promising technique for assessing the status of the skin without a surgical procedure. The capability of the modality to image microvasculature, visualize tumor boundaries, detect metastases in lymph nodes, perform fast and label-free histology, and identify CTCs could aid in the early diagnosis and classification of CM, including determination of metastatic status. In addition, it could be useful for monitoring treatment efficacy noninvasively.

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Section: Methodsmentioning

confidence: 99%

“…Our primary source was Google Scholar, which yielded 12,300 total results. Of the 12,300 results, 102 studies 103 , 109 , 113 , 115 – 117 , 132 – 140 , 165 – 245 met our criteria and are reviewed here.…”

Section: Methodsmentioning

confidence: 99%

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Fakhoury,

Lara,

Manwar

et al. 2024

J. Biomed. Opt.

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“…Supercontinuum (SC) sources have been increasingly applied for multi-wavelength excitation in PAM. 47 – 52 The use of highly nonlinear fibers, such as photonic crystal fibers, broadens the output light spectrum to hundreds of nanometers, which leads to a low energy density within each specific band. Recently, Chang et al.…”

Section: Light Sourcesmentioning

confidence: 99%

“…Supercontinuum (SC) sources have been increasingly applied for multi-wavelength excitation in PAM. [47][48][49][50][51][52] The use of highly nonlinear fibers, such as photonic crystal fibers, broadens the output light spectrum to hundreds of nanometers, which leads to a low energy density within each specific band. Recently, Chang et al reported the use of a commercial SC source with a PRR of up to 1 MHz for multispectral PAM and OCT. 17 Two wavelength bands around 528 nm and 558 nm light with a 10 nm bandwidth were used to image sO 2 of the mouse ear.…”

Section: Multi-wavelength Excitationmentioning

confidence: 99%

Sounding out the dynamics: a concise review of high-speed photoacoustic microscopy

Cho,

Nguyen,

DiSpirito

et al. 2024

J. Biomed. Opt.

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. Significance Photoacoustic microscopy (PAM) offers advantages in high-resolution and high-contrast imaging of biomedical chromophores. The speed of imaging is critical for leveraging these benefits in both preclinical and clinical settings. Ongoing technological innovations have substantially boosted PAM’s imaging speed, enabling real-time monitoring of dynamic biological processes. Aim This concise review synthesizes historical context and current advancements in high-speed PAM, with an emphasis on developments enabled by ultrafast lasers, scanning mechanisms, and advanced imaging processing methods. Approach We examine cutting-edge innovations across multiple facets of PAM, including light sources, scanning and detection systems, and computational techniques and explore their representative applications in biomedical research. Results: This work delineates the challenges that persist in achieving optimal high-speed PAM performance and forecasts its prospective impact on biomedical imaging. Conclusions Recognizing the current limitations, breaking through the drawbacks, and adopting the optimal combination of each technology will lead to the realization of ultimate high-speed PAM for both fundamental research and clinical translation.

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“…Fiber lasers are promising alternatives for PA technology because they have the potential to deliver both high pulse energy and robust performance against environmental conditions. Driven by these advantages, mature visible and NIR fiber laser technologies have been recently proposed for PA technology [17][18][19][20]. However, MIR high-energy fiber lasers have not yet been used for PAM.…”

Section: Introductionmentioning

confidence: 99%

Mid-infrared photoacoustic brain imaging enabled by cascaded gas-filled hollow-core fiber lasers

Zhang,

Sui,

Meneghetti

et al. 2024

Preprint

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SignificanceExtending the photoacoustic microscopy (PAM) into the mid-infrared (MIR) molecular fingerprint region constitutes a promising route towards label-free imaging of biological molecular structures. Realizing this objective requires a high-energy nano-second MIR laser source. However, existing MIR laser technologies are limited to either low pulse energy or free-space structure which is sensitive to environmental conditions. Fiber lasers are promising technologies for PAM for their potential of offering both high pulse energy and robust performance against environmental conditions. However, MIR high energy fiber laser has not yet been used for PAM because it is still at the infant research stage.AimWe aim to employ the emerging gas-filled anti-resonant hollow-core fiber (ARHCF) laser technology for MIR-PAM for the purpose of imaging myelin-rich regions in a mouse brain.ApproachThis laser source is developed with a ∼2.75 μJ high-pulse-energy nano-second laser at 3.4 μm, targeting the main absorption band of myelin sheaths, the primary chemical component of axons in the central nervous system. The laser mechanism relies on two-orders gas-induced vibrational stimulated Raman scattering (SRS) for nonlinear wavelength conversion, starting from a 1060 nm pump laser to 1409 nm through the 1storder Stokes generation in the nitrogen-filled 1ststage ARHCF, then, from 1409 nm to 3.4 μm through the 2ndstage hydrogen-filled ARHCF.ResultsThe developed Raman laser was used for the first time for transmission-mode MIR-PAM of mouse brain regions containing rich myelin structures.ConclusionsThis work pioneers the potential use of high-energy and nano-second gas-filled ARHCF laser source to MIR-PAM, with a first attempt to report this kind of fiber laser source for PAM of lipid-rich myelin regions in a mouse brain. The proposed ARHCF laser technology is also expected to generate high-energy pulses at the ultraviolet (UV) region, which can significantly improve the lateral resolution of the PAM.

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Efficient label-free in vivo photoacoustic imaging of melanoma cells using a condensed NIR-I spectral window

Cited by 11 publications

References 86 publications

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review

Sounding out the dynamics: a concise review of high-speed photoacoustic microscopy

Mid-infrared photoacoustic brain imaging enabled by cascaded gas-filled hollow-core fiber lasers

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