“…Thus, when using hard cut-offs in tracheal diameter, it is difficult to know how much of total tracheal volume that actually is quantified, and how much is found in trachea and tracheoles with smaller diameters. Future studies could explore possibilities to improve minimum resolution, for instance using machine learning algorithms that improve capacity to distinguish tracheal structures in fuzzy images (Yang et al, 2020). More generally, it would be important to study insect species across a wide range of body sizes to determine the distribution of tracheal size relationships (Aitkenhead et al, 2020;Kaiser et al, 2007) at different scanning resolutions (Iwan et al, 2015).…”