Efficient manipulation of microparticles in bubble streaming flows

Wang, Cheng; Jalikop, Shreyas V.; Hilgenfeldt, Sascha

doi:10.1063/1.3654949

Cited by 94 publications

(102 citation statements)

References 48 publications

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“…They are greatly beneficial in a number of proposed applications in chemical, biological, and physical processes such as mixing, [149][150][151] pumping, 152,153 characterizing enzymatic reactions, 154 cell lysis, 155 and sorting and enrichment. 156,157 A thorough review article on applications of oscillating microbubbles in microfluidics is recently written by Hashmi et al 158 Although, there are a number of techniques to generate bubbles in microfluidic channels, 159,160 using a cavity is found to be the simplest. Upon generation of the bubble, a piezoelectric transducer can be coupled with the device to oscillate the bubble.…”

Section: A Cavitation Microstreamingmentioning

confidence: 99%

“…165 The maximum streaming velocity, which is located near the bubble, can then be approximated from u s $ 2p 2 af . 155 However, in high applied voltages and typical applied frequencies in microfluidic systems 156,157 (15 kHz < f < 100 kHz), Re s $ 1 which makes the theoretical solution more complicated as the full Navier-Stokes equations must be solved. Nevertheless, RNW streaming can describe the flow in these cases quite accurately.…”

Section: A Cavitation Microstreamingmentioning

confidence: 99%

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Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturized diagnostic devices and rare cell enrichment techniques. Due to inherent problems of isolation methods based on the biomarkers and antigens, separation approaches exploiting physical characteristics of cells of interest, such as size, deformability, and electric and magnetic properties, have gained currency in many medical assays. Here, we present an overview of the cell/particle sorting techniques by harnessing intrinsic hydrodynamic effects in microchannels. Our emphasis is on the underlying fluid dynamical mechanisms causing cross stream migration of objects in shear and vortical flows. We also highlight the advantages and drawbacks of each method in terms of throughput, separation efficiency, and cell viability. Finally, we discuss the future research areas for extending the scope of hydrodynamic mechanisms and exploring new physical directions for microfluidic applications.

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Section: A Cavitation Microstreamingmentioning

confidence: 99%

Section: A Cavitation Microstreamingmentioning

confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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“…This will allow perform label-free imaging of the captured bio-targets using our device with a higher sensitivity as that can be achieved using the sensing mechanism described here and in our earlier work. 6,7 Wang et al 33 investigated the inertial effects due to vortical flow separation and due to the particles in such flow and found that oscillating microbubbles driven by ultrasound can initiate a steady streaming flow around the bubbles. This flow affects the microspheres' movement to exhibit size-dependent behaviors.…”

Section: E Comparison With Other Hydrodynamic Mechanismsmentioning

confidence: 99%

Optimization of microfluidic microsphere-trap arrays

Sarder

et al. 2013

Biomicrofluidics

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Microarray devices are powerful for detecting and analyzing biological targets. However, the potential of these devices may not be fully realized due to the lack of optimization of their design and implementation. In this work, we consider a microsphere-trap array device by employing microfluidic techniques and a hydrodynamic trapping mechanism. We design a novel geometric structure of the trap array in the device, and develop a comprehensive and robust framework to optimize the values of the geometric parameters to maximize the microsphere arrays' packing density. We also simultaneously optimize multiple criteria, such as efficiently immobilizing a single microsphere in each trap, effectively eliminating fluidic errors such as channel clogging and multiple microspheres in a single trap, minimizing errors in subsequent imaging experiments, and easily recovering targets. We use finite element simulations to validate the trapping mechanism of the device, and to study the effects of the optimization geometric parameters. We further perform microsphere-trapping experiments using the optimized device and a device with randomly selected geometric parameters, which we denote as the un-optimized device. These experiments demonstrate easy control of the transportation and manipulation of the microspheres in the optimized device. They also show that the optimized device greatly outperforms the un-optimized device by increasing the packing density by a factor of two, improving the microsphere trapping efficiency from 58% to 99%, and reducing fluidic errors from 48% to a negligible level (less than 1%). The optimization framework lays the foundation for the future goal of developing a modular, reliable, efficient, and inexpensive lab-on-a-chip system.

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“…3,4 Particles are passed through a channel with a flow field driven by oscillating bubbles and pressure. The flow field becomes a combination of closed and open streamlines.…”

mentioning

confidence: 99%

“…The mechanism of the trapping and sorting arises from the differences between interactions of the particles with the fluid. [2][3][4][5][6][7][8] In particular, counter-rotating vortices have been used to sort particles and manipulate biopolymers. They have been used to deposit DNA precisely across electrodes 9 and trap DNA.…”

mentioning

confidence: 99%

Separation of DNA by length in rotational flow: Lattice-Boltzmann-based simulations

2015

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We use a lattice-Boltzmann based Brownian dynamics simulation to investigate the separation of different lengths of DNA through the combination of a trapping force and the microflow created by counter-rotating vortices. We can separate most long DNA molecules from shorter chains that have lengths differing by as little as 30%. The sensitivity of this technique is determined by the flow rate, size of the trapping region, and the trapping strength. We expect that this technique can be used in microfluidic devices to separate long DNA fragments that result from techniques such as restriction enzyme digests of genomic DNA. V C 2015 AIP Publishing LLC.[http://dx.doi.org/10.1063/1.4926667] The development of novel methods for manipulating biopolymers such as DNA is required for the continued advancement of microfluidic devices. Techniques such as restriction enzyme digests for genomic sequencing rely on the detection of DNA that differ in length by sometimes thousands of base pairs. 1 Methods that separate DNA strands with resolutions on the order of kilobase pairs are required to analyze the products of this technique. To gain an insight into possible techniques to separate polymers, it can be helpful to review the methods to separate particles in microfluidic devices. Experimental work has shown how hydrodynamic mechanisms can lead to separation of particles based on size and deformability. 2 Eddies, microvortices, and hydrodynamic tweezers have been used to trap and sort particles. The mechanism of the trapping and sorting arises from the differences between interactions of the particles with the fluid. [2][3][4][5][6][7][8] In particular, counter-rotating vortices have been used to sort particles and manipulate biopolymers. They have been used to deposit DNA precisely across electrodes 9 and trap DNA. 10,11 Vortex flow may therefore be a good basis for a technique for sorting DNA by length.Streaming flow has been used in experiments to separate colloids of different size. Previous work on DNA has shown that counter-rotating vortices can be used to trap DNA dynamically. Long strands of DNA have been observed to stretch between the centers of two counter-rotating vortices. The polymer stays trapped in this state for significant amounts of time. 12 In a different experiment, the vortices were used to thermally cycle the polymer and allow replication via the polymerase chain reaction (PCR). The DNA is also trapped against one wall by a thermophoretic force in these experiments. 10 The strength of the trap is controlled by the gradient in temperature created by a focused infrared laser beam.Trapping DNA at one wall by counter-rotating vortices has also been explored in simulation and found to depend on the Peclet number, Pe ¼ u max L/D m , where u max is the maximum speed of the vortex, L is the box size, and D m is the diffusion coefficient of one bead in the

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Efficient manipulation of microparticles in bubble streaming flows

Cited by 94 publications

References 48 publications

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Optimization of microfluidic microsphere-trap arrays

Separation of DNA by length in rotational flow: Lattice-Boltzmann-based simulations

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