Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS

Diéguez, Lorena; Winter, Marnie; Pocock, Kyall; Bremmell, Kristin Elizabeth; Thierry, Benjamin

doi:10.1039/c4an01768d

Cited by 47 publications

(41 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, a rather complete depletion of white blood cells can be achieved by the introduction of a single-step treatment of the cells with sulfuric acid, creating a certain nanoscale roughness, which results in an increased binding of CD45-positive white blood cells to CD45-conjugated surfaces. Thereby isolation and characterization of tumor cells is increased [76]. A novel EpCAM independent enrichment strategy was proposed by Schneck et al in 2015: they combined different antibodies specific for cell surface proteins and components of the extracellular matrix (ECM) components.…”

Section: New Ctc-detection Strategiesmentioning

confidence: 99%

The Significance of Epithelial-to-Mesenchymal Transition for Circulating Tumor Cells

Kölbl

Jeschke

Andergassen

2016

IJMS

View full text Add to dashboard Cite

Epithelial to mesenchymal transition (EMT) is a process involved in embryonic development, but it also plays a role in remote metastasis formation in tumor diseases. During this process cells lose their epithelial features and adopt characteristics of mesenchymal cells. Thereby single tumor cells, which dissolve from the primary tumor, are enabled to invade the blood vessels and travel throughout the body as so called “circulating tumor cells” (CTCs). After leaving the blood stream the reverse process of EMT, the mesenchymal to epithelial transition (MET) helps the cells to seed in different tissues, thereby generating the bud of metastasis formation. As metastasis is the main reason for tumor-associated death, CTCs and the EMT process are in the focus of research in recent years. This review summarizes what was already found out about the molecular mechanisms driving EMT, the consequences of EMT for tumor cell detection, and suitable markers for the detection of CTCs which underwent EMT. The research work done in this field could open new roads towards combating cancer.

show abstract

Section: New Ctc-detection Strategiesmentioning

confidence: 99%

The Significance of Epithelial-to-Mesenchymal Transition for Circulating Tumor Cells

Kölbl

Jeschke

Andergassen

2016

IJMS

View full text Add to dashboard Cite

show abstract

“…The acidic treatment inside the PDMS channels of microfluidic chips was attempted to increase the purity of CTCs. A high‐throughput microfluidic chip with anti‐CD45 conjugated on the channel surface and roughening using Sulphur acid, achieved 99.99% WBCs capturing and 50% CTCs recovery (Diéguez, Winter, Pocock, Bremmell, & Thierry, ). Similar CTC enriching PDMS‐based membranes achieved the capture efficiency of 72.3 to 83% (Kang, Doh, & Cho, ; Zhou et al, ).…”

Section: Microfilter Structures and Fabrication Methodsmentioning

confidence: 99%

“…Track etched and cartridge filter ISET, ScreenCell (Desitter et al, 2011;Diéguez, Winter, Pocock, Bremmell, & Thierry, 2015;Fleischer, Price, & Symes, 1964;Kang, Doh, & Cho, 2015;Seal, 1964;Zhou et al, 2014) 2 Lithography CellSieve, Microfilter devices (Adams et al, 2014;Bikel et al, 2010;Chou et al, 2013;Girones et al, 2006;Mohamadi et al, 2015) 3…”

Section: Sr No Fabrication Techniquesmentioning

confidence: 99%

Filter‐based isolation, enrichment, and characterization of circulating tumor cells

Khetani

Mohammadi

Nezhad

2018

Biotech & Bioengineering

View full text Add to dashboard Cite

Isolation of circulating tumor cells (CTCs) from blood has long been a challenge due to the rarity and heterogeneity of these cells. Detection technologies have predominantly focused on different molecular or physical properties of CTCs. Size-based isolation approach using microfilters have been widely used to capture CTCs because of the difference in size and stiffness of the cells compared to other hemocytes. Isolation of rare cells based on their size was the original CTC enrichment technique and it demonstrated a simple yet rapid method that enhanced the recovery of cells with high throughput. In this review, we highlight key technical aspects of filter-based isolation, detection, and characterization of CTCs, and compare the clinical performance of filter-based devices with the approved platforms and immunoassays used for the analysis of CTCs. We have also discussed future prospective and incorporation of advances in immunochemistry technique into the filter-based platforms for enhancing the utility in clinical settings.

show abstract

“…MCF7 is the most frequently used breast cancer cell line worldwide . This cell line has been used as a CTC model in studies that isolate CTC from blood cells . Moreover, numerous studies have been reported showing that red blood cells are removed from blood samples by lysis or a similar pretreatment to facilitate the separation of CTCs from blood cells .…”

Section: Introductionmentioning

confidence: 99%

Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes

2020

View full text Add to dashboard Cite

The detection of circulating tumor cells (CTCs) in blood is crucial to assess metastatic progression and to guide therapy. Dielectrophoresis (DEP) is a powerful cell surface marker‐free method that allows intrinsic dielectric properties of suspended cells to be exploited for CTC enrichment/isolation from blood. Design of a successful DEP‐based CTC enrichment/isolation system requires that the DEP response of the targeted particles should accurately be known. This paper presents a DEP spectrum method to investigate the DEP spectra of cells without directly analyzing their membrane and cytoplasmic properties in contrast to the methods in literature, which employ theoretical assumptions and complex modeling. Integrating electric field simulations based on DEP theory with the experimental data enables determination of the DEP spectra of leukocyte subpopulations, polymorphonuclear and mononuclear leukocytes, and MCF7 breast cancer cells as a model of CTC due to their metastatic origin over the frequency range 100 kHz–50 MHz at 10 Vpp. In agreement with earlier findings, differential DEP responses were detected for mononuclear and polymorphonuclear leukocytes due to the richness of the cell surface features and morphologies of the different leukocyte types. The data reveal that the strength of the DEP force exerted on MCF7 cells was particularly high between 850 kHz and 20 MHz. These results illustrate that the proposed technique has the potential to provide a generic platform to identify DEP responses of different biological particles.

show abstract

Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS

Cited by 47 publications

References 30 publications

The Significance of Epithelial-to-Mesenchymal Transition for Circulating Tumor Cells

The Significance of Epithelial-to-Mesenchymal Transition for Circulating Tumor Cells

Filter‐based isolation, enrichment, and characterization of circulating tumor cells

Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes

Contact Info

Product

Resources

About