Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia

Ohtsubo, Norihiro; Sasaki, Keiko; Aida, Ryutaro; Ryuto, Hiromichi; Ichida, Hiroyuki; Hayashi, Yasuhiro; Abe, Tomoko

doi:10.1007/978-1-61779-558-9_24

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…On the other hand, heavy-ion beam irradiation has also led to a large variety of colors in Torenia flowers (Sasaki et al 2008). Thus, combination of chimeric repressors and heavy-ion beam irradiation (Ohtsubo et al 2012) could possibly enable the creation of a wide variety of colorful flowers with new floral traits.…”

Section: Fig 3 Simplified Flow Of the Ct Systemmentioning

confidence: 99%

Promotion of Efficient Molecular Breeding Using Chimeric Repressors in Ornamental Flowers

Sasaki

Ohtsubo

2016

JARQ

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Useful novel floral traits have recently been generated in various ornamental crops by using Chimeric REpressor gene Silencing Technology (CRES-T). CRES-T is a plant-specific gene silencing method that targets transcription factors (TFs), causing a dominant loss-of-function phenotype. In our group project, we applied CRES-T to various ornamental crops and mainly elucidated the following points: 1) CRES-T was effective for the modification of floral traits, 2) it was useful in higher polyploidy crops, and 3) it enabled the creation of commercially valuable flowers. In CRES-T, the attachment of a short repression domain derived from a transcriptional repressor to the C-terminal region of target TFs converts transcriptional activators to dominant chimeric repressors. In the target flower species, some Arabidopsis chimeric repressors were effective for the production of new floral traits without cloning the TF genes of interest. Therefore, the use of Arabidopsis chimeric repressors could be widely effective for many ornamental crops, even when the genome and/or expressed sequence tag (EST) information of target crops is unavailable or lacking. In addition, the collective transformation of chimeric repressors into ornamental crops is effective in isolating useful and/or target floral traits, such as petal colors, petal shapes, and color patterns. This technology could help to accelerate the developmental period and reduce the cost of developing new floral traits.

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Section: Fig 3 Simplified Flow Of the Ct Systemmentioning

confidence: 99%

Promotion of Efficient Molecular Breeding Using Chimeric Repressors in Ornamental Flowers

Sasaki

Ohtsubo

2016

JARQ

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“…Mutant phenotypes were observed mainly in flowers and showed wide variation in color and shape [48]. He and his coworkers succeeded in producing over 200 varieties of transgenic Torenia (Torenia fournieri Lind.)…”

Section: Photoperiodsmentioning

confidence: 99%

Plant Tissue Culture

Bhatia¹

2015

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

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“…Finally, it should be taken into account that, in addition to genetic engineering based on gene delivery, pigment mutations are also produced in torenia by heavy ion beam irradiation [51-53]. Moreover, mutations of petal color are also caused by gamma irradiation [54].…”

Section: Introductionmentioning

confidence: 99%

Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering

et al. 2013

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Advances in research in the past few years on the ornamental plant torenia (Torenia spps.) have made it notable as a model plant on the frontier of genetic engineering aimed at studying ornamental characteristics and pest control in horticultural ecosystems. The remarkable advantage of torenia over other ornamental plant species is the availability of an easy and high-efficiency transformation system for it. Unfortunately, most of the current torenia research is still not very widespread, because this species has not become prominent as an alternative to other successful model plants such as Arabidopsis, snapdragon and petunia. However, nowadays, a more global view using not only a few selected models but also several additional species are required for creating innovative ornamental traits and studying horticultural ecosystems. We therefore introduce and discuss recent research on torenia, the family Scrophulariaceae, for secondary metabolite bioengineering, in which global insights into horticulture, agriculture and ecology have been advanced. Floral traits, in torenia particularly floral color, have been extensively studied by manipulating the flavonoid biosynthetic pathways in flower organs. Plant aroma, including volatile terpenoids, has also been genetically modulated in order to understand the complicated nature of multi-trophic interactions that affect the behavior of predators and pollinators in the ecosystem. Torenia would accordingly be of great use for investigating both the variation in ornamental plants and the infochemical-mediated interactions with arthropods.

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Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia

Cited by 4 publications

References 10 publications

Promotion of Efficient Molecular Breeding Using Chimeric Repressors in Ornamental Flowers

Promotion of Efficient Molecular Breeding Using Chimeric Repressors in Ornamental Flowers

Plant Tissue Culture

Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering

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