Efficient mRNA delivery with graphene oxide-polyethylenimine for generation of footprint-free human induced pluripotent stem cells

Choi, Hye Yeon; Lee, Tae-Jin; Yang, Gwang-Mo; Oh, Jee Young; Won, Jihye; Han, Jihae; Jeong, Gajin; Kim, Jong-Pil; Kim, Jin‐Hoi; Kim, Byung Soo; Cho, Ssang-Goo

doi:10.1016/j.jconrel.2016.06.007

Cited by 100 publications

(72 citation statements)

References 63 publications

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“…In vivo, PEI-GO/pDNA VEGF complexes where injected in the peri-infarcted area in a rat model of myocardial infarction, leading to a significant increase in the number of microcapillaries in the area of injection, together with a reduction in scar size and an improvement in cardiac function compared to controls. Choi et al have not only been first to demonstrate mRNA complexation and efficient delivery by a GO-PEI construct, but also to report the generation of putative induced pluripotent stem (iPS) cells following such a strategy 67 . Delivery of synthetic mRNA or total RNA extracted from pluripotent stem cells generated cell colonies that expressed pluripotency markers, showed a pattern of DNA methylation similar to that of pluripotent cells and were able to differentiate in vitro towards all three germ-layers.…”

Section: Intracellular Molecular Sensingmentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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Section: Intracellular Molecular Sensingmentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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“…Moreover, Choi et al (2016) created GO-PEI complexes for the efficient generation of "footprint-free" induced pluripotent stem cells to avoid mRNA degradation. After the administration of GO-PEI/RNA complexes, the cells showed enhanced reprogramming efficiency and well-developed rat and human iPSCs from adult adipose tissue-derived fibroblasts, with no need for repetitive daily transfection (Choi et al, 2016). More recently, nontoxic graphene quantum dots were synthesized with two distinct (green and red) emission colors (Ghafary, Nikkhah, Hatamie, & Hosseinkhani, 2017 Overall functionalized graphene-based delivery systems showed superior characteristics in many of the drug-and gene-delivery studies (Figure 2).…”

Section: Gene Deliverymentioning

confidence: 99%

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Mohajeri

Behnam

Sahebkar

2018

Journal Cellular Physiology

214

100

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One of the major components in the development of nanomedicines is the choice of the right biomaterial, which notably determines the subsequent biological responses. The popularity of carbon nanomaterials (CNMs) has been on the rise due to their numerous applications in the fields of drug delivery, bioimaging, tissue engineering, and biosensing. Owing to their considerably high surface area, multifunctional surface chemistry, and excellent optical activity, novel functionalized CNMs possess efficient drug-loading capacity, biocompatibility, and lack of immunogenicity. Over the past few decades, several advances have been made on the functionalization of CNMs to minimize their health concerns and enhance their biosafety. Recent evidence has also implied that CNMs can be functionalized with bioactive peptides, proteins, nucleic acids, and drugs to achieve composites with remarkably low toxicity and high pharmaceutical efficiency. This review focuses on the three main classes of CNMs, including fullerenes, graphenes, and carbon nanotubes, and their recent biomedical applications.

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“…In the same year, cocktails of mRNA encoding Yamanaka factors were also used to generate iPSCs, followed by transfection with MyoD mRNA to induce further differentiation into myogenic cells [208]. Subsequently, other researchers attempted to improve the mRNA-based reprogramming technology by optimizing the transcript factor cocktails [209] or employing graphene oxide-polyethylenimine as the mRNA delivery system [210]. …”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical applications of mRNA nanomedicine

et al. 2018

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As an attractive alternative to plasmid DNA, messenger RNA (mRNA) has recently emerged as a promising class of nucleic acid therapeutics for biomedical applications. Advances in addressing the inherent shortcomings of mRNA and in the development of nanoparticle-based delivery systems have prompted the development and clinical translation of mRNA-based medicines. In this review, we discuss the chemical modification strategies of mRNA to improve its stability, minimize immune responses, and enhance translational efficacy. We also highlight recent progress in nanoparticle-based mRNA delivery. Considerable attention is given to the increasingly widespread applications of mRNA nanomedicine in the biomedical fields of vaccination, protein-replacement therapy, gene editing, and cellular reprogramming and engineering.

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Efficient mRNA delivery with graphene oxide-polyethylenimine for generation of footprint-free human induced pluripotent stem cells

Cited by 100 publications

References 63 publications

Graphene materials as 2D non-viral gene transfer vector platforms

Graphene materials as 2D non-viral gene transfer vector platforms

Biomedical applications of carbon nanomaterials: Drug and gene delivery potentials

Biomedical applications of mRNA nanomedicine

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