Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch

Yan, Xiangqiao; Wang, Kejia; Zheng, Keyuan; Zhang, Lifeng; Yang, Yong; Liu, Qi; Zhu, Minhao

doi:10.48130/fr-2023-0004

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…We observed both high and low induction rates at a 6-BA/NAA ratio of 3/1. The intensity of PGRs was also important; as the intensity of hormone combinations of the same ratio increased, shoot regeneration was first increased and then decreased; this phenomenon has also been reported during in vitro regeneration in other species (Shekhawat et al, 2021), indicating that both hormone combinations of ratio and intensity are important for the shoot regeneration rate (Yan et al, 2023). The in vitro regeneration of non-model plants typically relies on gradient hormone combinations or empirical experiments.…”

Section: Discussionmentioning

confidence: 75%

“…Our findings indicate that the choice of culture medium significantly impacts plantlet elongation and health, which formed different growth states and leaf colors: MS and 1/2MS media resulted in smaller adventitious shoots with curled, green leaves; WPM medium facilitated better shoot elongation and healthier green leaves, whereas DCR and 2× DCR media also supported shoot elongation but led to yellow and red leaf coloration, respectively. Studies on cultivated woody plants with obvious stems, such as Eucalyptus benthamii explant sources ( Esposito-Polesi et al., 2020 ), Larix olgensis ( Yan et al., 2023 ), Juniper species ( Hazubska-Przybył, 2019 ), and Persea americana Mill. cv.…”

Section: Discussionmentioning

confidence: 99%

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An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Yan,

Zheng,

et al. 2024

Front. Plant Sci.

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Bretschneidera sinensis is a monotypic species of rare and tertiary relic trees mainly distributed in China. B. sinensis is a potentially valuable horticultural plant, which has significant ornamental and research value, and is a crucial tool for the study of phylogeography. The artificial cultivation of B. sinensis is of great scientific value and practical significance. In this study, we developed a direct organogenesis process of B. sinensis using mature zygotic embryos as initial materials. The highest sterile germination induction (54.5%) from the mature zygotic embryo was obtained in a Murashige and Skoog (MS) medium with 2.0 mg·L−1 6-benzylaminopurine (6-BA) and 0.2 mg·L−1 α-naphthaleneacetic acid (NAA). The highest percentage of shoot regeneration (90.37%) was attained using 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA in the MS medium. The Woody Plant Medium (WPM) had the greatest adventitious shoot elongation rate of 93.33%. The most optimized rooting rate was 88.89% in a half-strength MS medium containing 2.0 mg·L−1 indole-3-butyric acid (IBA) and 1.0 mg·L−1 NAA. The genetic fidelity of in vitro regenerated plantlets was assessed using inter-simple sequence repeats and random amplified polymorphic DNA molecular markers, confirming the genetic uniformity and stability of regenerated B. sinensis plantlets. Our research presents an effective in vitro propagation system for B. sinensis, laying the groundwork for its germplasm conservation and large-scale production while maintaining high genetic integrity.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Yan,

Zheng,

et al. 2024

Front. Plant Sci.

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“…Larch are hardy, cold-resistant trees that grow rapidly and are resistant to decay. Larch wood can therefore be used for the construction of bridges and poles for example, while it also serves as a raw material for the wood fiber industry [ 1 ]. It dominates the world’s commercial forests and forest resources [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

H2O2 Significantly Affects Larix kaempferi × Larix olgensis Somatic Embryogenesis

Zhu,

Zhang,

Xiong

et al. 2024

IJMS

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Larch is widely distributed throughout the world and is an important species for timber supply and the extraction of industrial raw materials. In recent years, the hybrid breeding of Larix kaempferi and Larix olgensis has shown obvious heterosis in quick-growth, stress resistance and wood properties. However, its growth and development cycle is too long to meet general production needs. In order to shorten the breeding cycle, we have for the first time successfully established and optimized a somatic embryogenesis system for Larix kaempferi × Larix olgensis. We found that the highest rate of embryonal-suspensor mass (ESM) induction was observed when late cotyledonary embryos were used as explants. The induced ESMs were subjected to stable proliferation, after which abscisic acid (ABA) and polyethylene glycol (PEG) were added to successfully induce somatic embryos. Treatment with PEG and ABA was of great importance to somatic embryo formation and complemented each other’s effect. ABA assisted embryo growth, whereas PEG facilitated the formation of proembryo-like structures. On top of this, we studied in more detail the relationship between redox homeostasis and the efficiency of somatic embryogenesis (frequency of ESM induction). During subculture, we observed the gradual formation of three distinct types of ESM. The Type I ESM is readily able to form somatic embryos. In contrast to type I, the type III ESM suffers from severe browning, contains a higher level of hydrogen peroxide (H2O2) and demonstrates a decreased ability to form somatic embryos. External treatment with H2O2 decreased the somatic embryogenesis efficiency of Type I and type III ESMs, or the higher the exogenous H2O2 content, the lower the resulting somatic embryogenesis efficiency. We found that treatment with the H2O2 scavenger DMTU (dimethylthiourea) could significantly increase the somatic embryogenesis efficiency of the type III ESM, as a result of a decline in endogenous H2O2 content. Overall, these findings have contributed to setting up a successful somatic embryogenesis system for larch production.

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“…At present, plant tissue culture technology is becoming more and more mature, and many poplar genotypes have established mature regeneration systems [ 16 , 17 , 18 ]. The regeneration of many woody plants (including poplar and conifer species) can be carried out in various ways, such as those involving organs, somatic embryos and protoplasts, which lay the foundation for the genetic study of woody plants [ 6 , 7 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar

Li,

Wang,

Guan

et al. 2023

Life

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A series of tissue culture regeneration protocols were conducted on gray poplar (P. tremula × P. alba) to select the most efficient callus induction medium, adventitious shoot differentiation medium, shoot elongation medium and rooting medium, which laid the foundation for the optimization of genetic transformation technology for gray poplar. The results showed that the Woody Plant Medium (WPM) supplemented with 0.10 mg L−1 kinetin (KT) and 1.00 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was the most suitable medium for callus induction. The callus induction rates of different tissues were greater than 85.7%. The optimal adventitious shoot differentiation medium was the WPM supplemented with 0.02 mg L−1 thidiazuron (TDZ), and the adventitious shoot differentiation rates of young tissues were 22.2–41.9%. The optimal direct differentiation medium was the Murashige and Skoog (MS) medium supplemented with 0.20 mg L−1 6-benzylaminopurine (6-BA), 0.10 mg L−1 indole butyric acid (IBA) and 0.001 mg L−1 TDZ, and the differentiation rate of adventitious shoots was greater than 94%. The best shoot elongation medium for adventitious shoots was the MS medium with 0.10 mg L−1 naphthylacetic acid (NAA). After 45 days of cultivation in the MS medium with 0.10 mg L−1 NAA, the average plant height was 1.8 cm, and the average number of elongated adventitious shoots was 11 per explant. The 1/2 MS medium with 0.10 mg L−1 NAA showed the best performance for rooting, and later, shoot growth. The direct shoot induction pathway can induce adventitious shoots much faster than the indirect adventitious shoot induction pathway can, and the time cost via the direct adventitious shoot induction pathway can be shortened by 2–6 weeks compared to that of the indirect shoot induction pathway.

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Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch

Cited by 4 publications

References 36 publications

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

H2O2 Significantly Affects Larix kaempferi × Larix olgensis Somatic Embryogenesis

Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar

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