Efficient plant regeneration and Agrobacterium-mediated transformation of Begonia semperflorens-cultorum

Hirutani, Sakiko; Shimomae, Kazuki; Yaguchi, Akira; Chin, Dong Poh; Mii, Masahiro; Igawa, Tomoko

doi:10.1007/s11240-020-01858-7

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…It is also the most used transgenic method for ornamental plants, including herbaceous, woody, bulb, and perennial root ornamental species (Table 1). Since adventitious bud regeneration is still the main regeneration pathway, the leaf is the most commonly used explant in ornamental plant transformation, while the protocorm is more used for bulbous species (Hoshi et al, 2004;Abbasi et al, 2020;Hirutani et al, 2020). Regeneration of woody species is still more challenging than that of other floral species; thus, the induction of somatic calli and the subsequent development of somatic embryos became an alternative method (Mishiba et al, 2005;Chin et al, 2007).…”

Section: Genetic Transformation System: the Base Of Genetic Engineeringmentioning

confidence: 99%

Creating novel ornamentals via new strategies in the era of genome editing

et al. 2023

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Ornamental breeding has traditionally focused on improving novelty, yield, quality, and resistance to biotic or abiotic stress. However, achieving these goals has often required laborious crossbreeding, while precise breeding techniques have been underutilized. Fortunately, recent advancements in plant genome sequencing and editing technology have opened up exciting new frontiers for revolutionizing ornamental breeding. In this review, we provide an overview of the current state of ornamental transgenic breeding and propose four promising breeding strategies that have already proven successful in crop breeding and could be adapted for ornamental breeding with the help of genome editing. These strategies include recombination manipulation, haploid inducer creation, clonal seed production, and reverse breeding. We also discuss in detail the research progress, application status, and feasibility of each of these tactics.

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Section: Genetic Transformation System: the Base Of Genetic Engineeringmentioning

confidence: 99%

Creating novel ornamentals via new strategies in the era of genome editing

et al. 2023

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“…No caso das variedades que compõem o híbrido Begonia semperflorens-cultorum Hort., estas plantas possuem um potencial de adaptação a diversos ambientes devido ao seu porte, rusticidade e vigor (PAULUS et al, 2016;ZHANG et al, 2018). De acordo com Hirutani et al, (2020), em comparação com outros híbridos, essas plantas conseguem se adaptar a diferentes condições de luminosidade e apresentam maior resistência à seca. Além disso, são fáceis de cultivar e requerem menos manutenção.…”

Section: Introdução Geralunclassified

Crescimento e anatomia de \'Begonia Megawatt\' (>i<Begonia x semperflorenscultorum>/i< Hort.) cultivadas sob diferentes telas de sombreamento

Oliveira

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Growth and anatomy of 'Begonia Megawatt' (Begonia x semperflorenscultorum Hort.) cultivated under different shading netsThe production of flowers and ornamental plants requires various technologies to enhance cultivation and ensure the control of meteorological variables, such as solar radiation. In this context, a technology that has been studied is the use of shading nets that provide better conditions for plant cultivation in a protected environment. The objective of this study is to evaluate the effect of using different shading nets on the growth and anatomical structure of two varieties of 'Begonia Megawatt': 'Pink Green' and 'Red Bronze'. The experiment was conducted in a greenhouse using a randomized complete block design with split-plot arrangement. The treatments consisted of combinations of blue, red, black, and control (without netting) representing the main plots, and the varieties 'Pink Green' and 'Red Bronze' representing the subplots. The growth responses were similar for both varieties. With the use of the nets, regardless of color, an increase in leaf area, number of leaves and inflorescences, higher flower and peduncle weight, and dry matter of flowers and peduncles were observed. Moreover, the nets provided a more favorable microclimate for cultivation, resulting in commercially appealing plants. The control treatment did not show significant differences in the analyzed variables, except for a more intense coloration of the tepals. Visually, the blue nets contributed to more compact plants, while the red nets resulted in more robust and vigorous plants. Regarding anatomical aspects, the blue and red nets promoted modifications in stomatal development, with well-developed clusters, larger and more numerous stomata per cluster; in the structure of the leaf blade, with larger and more organized mesophyll tissues and a higher amount of calcium oxalate; and in the roots, with larger and less numerous vessel elements. On the other hand, the control treatment resulted in smaller clusters with fewer stomata per cluster, smaller and disarranged mesophyll tissues, and roots with a higher number of vessel elements, larger area and diameter of the vascular cylinder and xylem. The black net showed intermediate results between the blue, red nets, and the control treatment.

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