Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]

Shimomae, Kazuki; Chin, Dong Poh; Khan, Raham Sher; Mii, Masahiro

doi:10.5511/plantbiotechnology.13.0721a

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…This evidence differed from other grasses. In many grass species, the efficiency of callus induction was affected in MS medium contained 2,4-D only or 2,4-D combined with low concentration of cytokinins [12]. This different result may be due to the difference of explant or the effect of cytokinin combination.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (<i>Vetiveria nemoralis </i>A. Camus cv. Roiet)

Khunchuay

Sompornpailin

2015

AMM

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The optimum ratios of auxin and cytokinin are necessary for callus induction and plant regeneration. This ratio is a key function involving in the promoting cell division and proliferation in tissue culture. The axillary buds of in vitro plantlets fromVetiveria nemoralisA. Camuscv. Roiet were used as explants for the callus induction experiment. These explants were cultured on Murashige & Skoog (MS) medium [1] supplemented with various combinations of auxins and cytokinins. Under this experimental study, the highest frequency of callus induction was found on MS medium supplemented with 2 mgL-1α-naphthalene acetic acid (NAA) and 1 mgL-12-furanylmethyl-1H-purine-6-amine (kinetin) (62.5%). On the other hand the combination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) was toxicity to this explants. All culturing explants were dead and no calli appearance. The calli derived from each medium were transferred into the same regeneration medium (MS with 1 mgL-1NAA and 2 mgL-1BAP). After culturing on regeneration medium, calli induced from the highest callus induction medium have shown high frequencies of regeneration and also shoot number per callus (58.33% and 7.1 shoots).

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Section: Resultsmentioning

confidence: 99%

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (<i>Vetiveria nemoralis </i>A. Camus cv. Roiet)

Khunchuay

Sompornpailin

2015

AMM

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“…Also, the relative water content of GP calli was found to be significantly less than D91, which shows that low relative water content is an important factor of somatic embryogenesis and regeneration. Previous reports demonstrated that out of 3 callus types of ravenna grass, calli with lowest water content showed highest callus proliferation, whereas the calli with highest water content showed lowest proliferation and regeneration (Shimomae et al;.…”

Section: Lignin Biosynthesis Is Important During Somatic Embryogenesismentioning

confidence: 90%

Chloroplast activity provides in vitro regeneration capability in contrasting cultivars

Sirohi

Chaudhary

Baliyan

et al. 2022

Preprint

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Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. We know little about why only some cultivars in crop plants are tissue culture friendly. In this study, tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency. Multiomics studies involving transcriptomics, proteomics, metabolomics, and biochemical analysis were performed using GP and D91 callus to unravel the regulatory mechanisms. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MSMS revealed 3062 protein groups and 16989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis also revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91.

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Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley

Sirohi,

Chaudhary,

Sharma

et al. 2024

Plant Mol Biol

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Efficient plant regeneration system from seed-derived callus of ravenna grass [Erianthus ravennae (L.) Beauv.]

Cited by 6 publications

References 20 publications

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (<i>Vetiveria nemoralis </i>A. Camus cv. Roiet)

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (<i>Vetiveria nemoralis </i>A. Camus cv. Roiet)

Chloroplast activity provides in vitro regeneration capability in contrasting cultivars

Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley

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