Efficient plant regeneration via meristematic nodule culture in Paeonia ostii ‘Feng Dan’

Xu, Li; Cheng, Fangyun; Zhong, Yuan

doi:10.1007/s11240-021-02216-x

Cited by 7 publications

(9 citation statements)

References 43 publications

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“…Histological examination con rmed this morphogenic response. This is contrary to previous conclusion that callus formation was necessary for MNs induction in tree peony (Xu et al 2022a). In fact, there were two morphogenesis pathways has been reported on MNs induction, direct (Moyo et al 2009;Ferreira et al 2009;Piéron et al 1998) and indirect with callus formation (Fortes and Pais 2000; Batista et al 2000).…”

Section: Discussioncontrasting

confidence: 99%

“…According to Xu et al (2022a), CPPU and TDZ, two kinds of cytokinin with high activity, played remarkably positive roles in MNs induction and shoots regeneration from callus. Meanwhile, DO and direct MNs induction only occurred in DM containing TDZ or CPPU in this research, but not in presence of BA, which further supported previous speculation that high level of cytokinin was indispensable for differentiation (Xu et al 2022a;Meng et al 2017). The result that TDZ + NAA, CPPU + NAA treatment in DIM had a better effect on the induction of MNs and DO than BA + NAA treatment also consistent with this conclusion.…”

Section: Discussionsupporting

confidence: 89%

“…In the present study, both of CPPU and TDZ concentration had signi cant effect on the frequency of DO (p<0.01), but TDZ showed no signi cant effect on the direct MN induction rate (p>0.05). Similar result has been concluded in tree peony that CPPU was considerably effective in promoting MNs induction from callus (Xu et al 2022a), and in herbaceous peony that TDZ was appropriate for direct organogenesis from cotyledon explants (Zhao et al 2017). Concurrently, the best performance of DO and direct MNs induction was presented on CPPU and TDZ combination at different level of concentration in our study, and higher level suitable for DO.…”

Section: Discussionsupporting

confidence: 83%

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Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

fan,

li,

et al. 2024

Preprint

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Tissue culture is preferred for solving the shortcoming of low efficiency in terms of conventional propagation ways in tree peony, an economically important woody plant in China with various purposes. However, callus differentiation is hard to obtain during in vitro regeneration. Meristematic nodule (MN) is a favorable way capable of overcoming this problem, but possesses a lengthy process. Direct organogenesis excluding the callus step is needed to simplify the procedure. This study firstly presented a protocol of direct organogenesis and direct MNs induction and differentiation using cotyledon explant for in vitro regeneration of P.ostii ‘Feng Dan’. The highest direct MNs induction rate (41.67%) and frequency of direct organogenesis (DO) (66.67%) was achieved under the following procedure. The explants were pretreated in dedifferentiation induction medium (DIM) [Murashige and Skoog (MS) medium with 2.27 µMthidiazuron (TDZ)+5.37 µM α-naphthylacetic acid (NAA)] for 10 days, and then the cotyledons without callus induced were transferred to differentiation medium (DM) [Woody plant medium (WPM) containing 2.02 µM N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU)+2.27 µM TDZ and 4.04 µM CPPU+4.54 µM TDZ] respectively, with 6 subcultures, 90 days in total. The regenerated shoots rooted and transplanted successfully. Histological study conﬁrmed the process of DO and direct MNs induction, and revealed that shoots and MNs were originated from increased division of meristematic cell under cortical tissue, as well as from actively divided meristematic cells around vascular center. Moreover, shoots regenerated through MNs differentiation were originated from the epidermal and subepidermal cells. This study is an innovation and supplement in the field of in vitro regeneration in tree peony, and will be conductive to clonal micropropagation, fundamental studies of developmental biology and genetic transformation.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 83%

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Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

fan,

li,

et al. 2024

Preprint

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“…No somatic embryogenesis was observed on any of the tested media combinations. Similar to a study on tree peony 15 , the regeneration of Xanthosoma involved four distinct phases that included callus with multiple meristematic regions, pre- nodules, meristematic nodules and shoot primordia. Formation of meristematic nodules either directly or indirectly via callogenesis has been reported in a number of species including Acacia mangium 16 , Eucalyptus globulus 17 , Humulus lupulus 18 , 19 , Populus euphratica 20 , Sclerocarya birrea 21 etc.…”

Section: Resultsmentioning

confidence: 95%

Histological and molecular insights in to in vitro regeneration pattern of Xanthosoma sagittifolium

Bansal

Sharma

Singh

et al. 2023

Sci Rep

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A study on the effect of various phytohormonal combinations on in vitro propagation of Cocoyam [Xanthosoma sagittifolium (L.) Schott] was conducted to develop an improved and efficient in vitro regeneration protocol for its mass multiplication. Histological analysis to understand the in vitro regeneration pattern and genetic fidelity assessment of regenerated plants were also carried out. Single shoots excised from in vitro established cultures of X. sagittifolium were used as explants. Among the 32 different phytohormonal combinations tested, indirect organogenesis with intervening callus phase was observed on majority of the media combinations. Meristematic clump formation was optimally achieved on all the tested media combinations with maximum 43.54 ± 0.51 shoot primordia on MS medium containing 0.2 mg/L BAP + 0.1 mg/L NAA followed by 36.44 ± 0.76 shoot primordia on MS medium having 2.5 mg/L TDZ. Micro-morphological analysis of different morphogenetic structures revealed that the regeneration of cocoyam is well executed via meristematic nodules, shoot primordia formation that may evolve in to proper shoots. Adventitious shoots (> 2 cm) were successfully (100.00 ± 0.00%) rooted on the half-strength MS medium containing IBA (0.05–1.0 mg/L) and IAA (0.05–0.5 mg/L). The number of roots ranged from 0.78 ± 0.31 on the control half-strength MS medium to 13.94 ± 0.46 on half-strength MS supplemented with 1.0 mg/L IBA. Considering somaclonal variations as a potential restriction to in vitro multiplication of plants, genetic stability was assessed using 40 ISSR primers. The PCR amplification profiles obtained from all the tested propagules (calli, meristematic clumps, regenerated plantlets) were similar to the mother plants indicating the homogeneity of the individuals raised through the regeneration protocol reported here.

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“…Similar results have been reported for S. hybrida [ 17 ] and S. plebeia [ 22 ]. Additionally, the ratio of cytokinin and auxin being applied can alter their interaction and impose a significant effect on shoot regeneration and proliferation [ 38 , 39 , 40 ]. We found that the combination of 3 mg L −1 cytokinin and 0.1 mg L −1 auxin was the most effective in promoting shoot proliferation.…”

Section: Discussionmentioning

confidence: 99%

Development of a Highly Efficient Shoot Organogenesis System for an Ornamental Aeschynanthus pulcher (Blume) G. Don Using Leaves as Explants

Yang

Yuan

et al. 2022

Plants

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Aeschynanthus pulcher (Blume) G. Don, the “lipstick plant” is a prized ornamental plant with distinctive flowers. Here, we introduce a novel in vitro regeneration method for A. pulcher using leaf explants and an optimized combination of phytohormone plant growth regulators (PGRs). The optimal conditions for shoot regeneration included 1 mg L−1 polyvinyl pyrrolidone (PVP) plus 3 mg L−1 thidiazuron (TDZ), inducing a response rate of 82.4% and a shoot/explant ratio of 38.6. When the Murashige and Skoog (MS) medium contained indole-3-butyric acid (IBA) alone, leaves first differentiated into adventitious roots and then adventitious shoots. Leaves cultured on MS medium containing 1 g L−1 PVP, 3 mg L−1 TDZ, 5 mg L−1 casein, and 0.1 mg L−1 α-naphthaleneacetic acid (NAA) for 30 d exhibited the highest embryogenic callus (EC) induction rate (95.6%). The optimal shoot proliferation coefficient (21.5) was obtained when shoots derived from EC were cultured on the same medium as that used for EC induction for 5 weeks. The most effective medium for rooting of elongated shoots was MS medium containing 1 g L−1 PVP, 5 mg L−1 casein, 3 mg L−1 6-benzyladenine (BA), and 0.1 mg L−1 NAA, and the number of roots reached 18.8. The regenerated plants grown in a greenhouse had 100% survival following one week of hardening. Overall, our effective and efficient propagation method should result in shortened culture periods and reduced production costs, allowing for the future selective breeding and genetic improvement of A. pulcher.

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Efficient plant regeneration via meristematic nodule culture in Paeonia ostii ‘Feng Dan’

Cited by 7 publications

References 43 publications

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

Paeonia ostii ‘Feng Dan’ plant regeneration through direct organogenesis and direct meristematic nodule culture

Histological and molecular insights in to in vitro regeneration pattern of Xanthosoma sagittifolium

Development of a Highly Efficient Shoot Organogenesis System for an Ornamental Aeschynanthus pulcher (Blume) G. Don Using Leaves as Explants

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