Efficient production of a lentiviral system for displaying Crimean-Congo hemorrhagic fever virus glycoproteins reveals a broad range of cellular susceptibility and neutralization ability

Vasmehjani, Abbas Ahmadi; Salehi‐Vaziri, Mostafa; Azadmanesh, Kayhan; Nejati, Ahmad; Pouriayevali, Mohammad Hassan; Gouya, MM; Parsaeian, Mahboubeh; Shahmahmoodi, Shohreh

doi:10.1007/s00705-020-04576-9

Cited by 3 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two serological assays consistently had the highest neutralization efficiency observed in the serum sample with the highest IgG titer, which was also mentioned in a previous study ( Vasmehjani et al., 2020 ). However, comparing the IgG antibody titers and NAbs in the activated animal sera involved in this study, we found that some sera after the first vaccination were positive in sVNT, whereas these sera were negative in the rGP-based ELISA, indicating an inconsistent correlation between IgG and neutralizing antibodies.…”

Section: Discussionsupporting

confidence: 86%

“…Moreover, the overall results of the two serological assays remained consistent in that the highest neutralization efficiency was observed in the mouse serum sample with the highest IgG titer, which was also mentioned in a previous study (Vasmehjani et al, 2020). However, comparing the results of rGP-based ELISA and sVNT, we also found that some sera were positive in sVNT, whereas these sera were negative in the rGP-based ELISA.…”

Section: Sd (Figuresupporting

confidence: 88%

See 1 more Smart Citation

Establishment of two serological methods for detecting IgG and neutralizing antibodies against Crimean-Congo hemorrhagic fever virus glycoprotein

Wang,

Shi

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

IntroductionThe Crimean-Congo hemorrhagic fever virus (CCHFV), the most geographically widespread tick-borne virus, is endemic in Africa, Eastern Europe and Asia, with infection resulting in mortality in up to 30% of cases. Currently, there are no approved vaccines or effective therapies available for CCHF. The CCHFV should only be manipulated in the BSL-4 laboratory, which has severely hampered basic seroprevalence studies. MethodsIn the present study, two antibody detection methods in the forms of an enzyme-linked immunosorbent assay (ELISA) and a surrogate virus neutralization test (sPVNT) were developed using a recombinant glycoprotein (rGP) and a vesicular stomatitis virus (VSV)-based virus bearing the CCHFV recombinant glycoprotein (rVSV/CCHFV) in a biosafety level 2 (BSL-2) laboratory, respectively. ResultsThe rGP-based ELISA and rVSV/CCHFV-based sVNT were established by using the anti-CCHFV pre-GC mAb 11E7, known as a broadly cross-reactive, potently neutralizing antibody, and their applications as diagnostic antigens were validated for the specific detection of CCHFV IgG and neutralizing antibodies in experimental animals. In two tests, mAb clone 11E7 (diluted at 1:163840 or 512) still displayed positive binding and neutralization, and the presence of antibodies (IgG and neutralizing) against the rGP and rVSV/CCHFV was also determined in the sera from the experimental animals. Both mAb 11E7 and animal sera showed a high reactivity to both antigens, indicating that bacterially expressed rGP and rVSV/CCHFV have good immunoreactivity. Apart from establishing two serological testing methods, their results also demonstrated an imperfect correlation between IgG and neutralizing antibodies. DiscussionWithin this limited number of samples, the rGP and rVSV/CCHFV could be safe and convenient tools with significant potential for research on specific antibodies and serological samples.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Sd (Figuresupporting

confidence: 88%

Establishment of two serological methods for detecting IgG and neutralizing antibodies against Crimean-Congo hemorrhagic fever virus glycoprotein

Wang,

Shi

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The CCHFV‐GP‐pseudotyped LV (Lenti‐CCHFV‐GP) was generated as described previously 18 . For the pseudotyped based neutralization assay (pVNT), plasma samples from CCHF–recovered patients were heat inactivated at 56°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The CCHFV-GP-pseudotyped LV (Lenti-CCHFV-GP) was generated as described previously. 18 For the pseudotyped based neutralization assay (pVNT), plasma samples from CCHF-recovered patients were heat inactivated at 56°C for 30 min. A twofold serial dilution of each serum was prepared and mixed with 100 000 Transducing units/mL (TU/mL) of Lenti-CCHFV-GP particles.…”

Section: Cchfv-gp-pseudotyped Lv-based Neutralization Assaymentioning

confidence: 99%

Persistence of IgG and neutralizing antibodies in Crimean−Congo hemorrhagic fever survivors

Vasmehjani,

Pouriayevali,

Shahmahmoodi

et al. 2024

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

The World Health Organization classified Crimean–Congo hemorrhagic fever (CCHF) as a high‐priority infectious disease and emphasized the performance of research studies and product development against it. Little information is available about the immune response due to natural CCHF virus (CCHFV) infection in humans. Here, we investigated the persistence of IgG and neutralizing antibodies in serum samples collected from 61 Iranian CCHF survivors with various time points after recovery (<12, 12−60, and >60 months after disease). The ELISA results showed IgG seropositivity in all samples while a pseudotyped based neutralization assay findings revealed the presence of neutralizing antibody in 29 samples (46.77%). For both IgG and neutralizing antibodies, a decreasing trend of titer was observed with the increase in the time after recovery. Not only the mean titer of IgG (772.80 U/mL) was higher than mean neutralizing antibody (25.64) but also the IgG persistence was longer. In conclusion, our findings provide valuable information about the long‐term persistence of humoral immune response in CCHF survivors indicating that IgG antibody can be detected at least 8 years after recovery and low titers of neutralizing antibody can be detected in CCHF survivors.

show abstract

Pseudotyped Viruses

Wang

Zhou

et al. 2023

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Efficient production of a lentiviral system for displaying Crimean-Congo hemorrhagic fever virus glycoproteins reveals a broad range of cellular susceptibility and neutralization ability

Cited by 3 publications

References 32 publications

Establishment of two serological methods for detecting IgG and neutralizing antibodies against Crimean-Congo hemorrhagic fever virus glycoprotein

Establishment of two serological methods for detecting IgG and neutralizing antibodies against Crimean-Congo hemorrhagic fever virus glycoprotein

Persistence of IgG and neutralizing antibodies in Crimean−Congo hemorrhagic fever survivors

Pseudotyped Viruses

Contact Info

Product

Resources

About