Efficient production of fungal spores by the combination of reduction of nitrogen source content and embedding of hydrophobic polymer in an agar plate

Sugimoto, Kyoko; Oda, Shinobu

doi:10.1016/j.jbiosc.2020.12.007

Cited by 4 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mycelial morphology and growth phenotypes of fungal isolates can be variably influenced by the composition of culture growth media. Previous work demonstrated that culture media composition can impact the formation of developmental structures in the fungal host, Moura et al (2020) , Sugimoto and Oda (2021) and that these changes coupled with predicted metabolic changes in the fungus could alter endobacterial phenotypes, abundance, and behavior. To determine the effects of culture media composition on endobacterial phenotypes, P. verticillata was grown on four different solid media: (R2A) media which is favorable for bacterial growth, Vogel’s minimal (VM) media for fungi, and two common fungal media, potato dextrose (PD), and malt extract (ME) media which provides a slightly more acidic media environment.…”

Section: Resultsmentioning

confidence: 99%

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

et al. 2022

View full text Add to dashboard Cite

Several bacteria have long been known to interact intimately with fungi, but molecular approaches have only recently uncovered how cosmopolitan these interactions are in nature. Currently, bacterial–fungal interactions (BFI) are inferred based on patterns of co-occurrence in amplicon sequencing investigations. However, determining the nature of these interactions, whether the bacteria are internally or externally associated, remains a grand challenge in BFI research. Fluorescence in situ hybridization (FISH) is a robust method that targets unique sequences of interest which can be employed for visualizing intra-hyphal targets, such as mitochondrial organelles or, as in this study, bacteria. We evaluate the challenges and employable strategies to resolve intra-hyphal BFI to address pertinent criteria in BFI research, such as culturing media, spatial distribution of bacteria, and abundance of bacterial 16S rRNA copies for fluorescent labeling. While these experimental factors influence labeling and detection of endobacteria, we demonstrate how to overcome these challenges thorough permeabilization, appropriate media choice, and targeted amplification using hybridization chain reaction FISH. Such microscopy imaging approaches can now be utilized by the broader research community to complement sequence-based investigations and provide more conclusive evidence on the nature of specific bacterial–fungal relationships.

show abstract

Section: Resultsmentioning

confidence: 99%

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

et al. 2022

View full text Add to dashboard Cite

show abstract

“…To induce spore production, polypropylene (PP) was mixed with PDA medium and inoculated with the fungal mycelium. The mycelium was gently swept with a sterilized brush, and variable-temperature incubation was applied alternatively between 24 h at 28 °C and 24 h at 4 °C [ 14 ]. Specimens of the dried cultures were deposited at the Herbarium of Guizhou Academy of Agricultural Sciences (Herb.…”

Section: Methodsmentioning

confidence: 99%

Insights from the Endophytic Fungi in Amphisphaeria (Sordariomycetes): A. orixae sp. nov. from Orixa japonica and Its Secondary Metabolites

et al. 2023

View full text Add to dashboard Cite

Endophytic fungi are a remarkably diverse group of microorganisms that have imperceptible associations with their hosts for at least a part of their life cycle. The enormous biological diversity and the capability of producing bioactive secondary metabolites such as alkaloids, terpenoids, and polyketides have attracted the attention of different scientific communities, resulting in numerous investigations on these fungal endophytes. During our surveys of plant-root-based fungi in the mountain areas of Qingzhen, Guizhou Province, several isolates of endophytic fungi were identified. In this study, a novel endophytic fungus was discovered in the roots of a medicinal plant (Orixa japonica) in Southern China and introduced as a new species (Amphisphaeria orixae) based on morphological evidence and molecular phylogenetic analysis (combined ITS and LSU sequence data). To the best of our knowledge, A. orixae is the first reported endophyte as well as the first hyphomycetous asexual morph in Amphisphaeria. A new isocoumarin, (R)-4,6,8-trihydroxy-5-methylisochroman-1-one (1), and 12 known compounds (2–13) were isolated from the rice fermentation products of this fungus. Using 1D- and 2D-NMR, mass spectrometry, and ECD studies, their structures were identified. The antitumor activity of these compounds was tested. Unfortunately, none of the compounds tested showed significant antitumor activity.

show abstract

Applying the first microcapsule-based self-healing microbial-induced calcium carbonate materials to prevent the migration of Pb ions

Xue,

Cheng,

Wang

et al. 2023

Environmental Research

View full text Add to dashboard Cite

Efficient production of fungal spores by the combination of reduction of nitrogen source content and embedding of hydrophobic polymer in an agar plate

Cited by 4 publications

References 37 publications

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

Insights from the Endophytic Fungi in Amphisphaeria (Sordariomycetes): A. orixae sp. nov. from Orixa japonica and Its Secondary Metabolites

Applying the first microcapsule-based self-healing microbial-induced calcium carbonate materials to prevent the migration of Pb ions

Contact Info

Product

Resources

About