Efficient production of hydroxymethyl-2-furfurylamine by chemoenzymatic cascade catalysis of bread waste in a sustainable approach

A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum

“…The biological reaction conditions have considerable influence on the biocatalytic activity. [ 31 ] Tracking the effect of reaction temperature, pH, and reaction time on catalytic efficiency enables optimal performance. The highest catalytic activity was obtained at 30°C (Figure 3A).…”

“…This may be due to how the increased shaking speed disrupts the structure of the enzyme. [ 31 ] When the system contains high‐titer substrates, the cell catalysts and glycosyl donors increase proportionally with respect to the substrate concentration making the interaction space between substrate and enzyme closer. Accordingly, the shaking speed and reaction time need to be adjusted to obtain highly efficient conversion.…”

Significantly enhanced bioconversion of high titer biomass-derived furfural to furfuryl alcohol by robust endogenous aldehyde reductase in a sustainable way

“…The sustainable manufacture of biofuran chemicals from abundant, inexpensive and renewable lignocellulose is crucial to achieving a clean biorefinery process. 58–60 One-pot two-step reaction combining non-enzymatic and enzymatic transformation provides a sustainable way to manufacture valuable biobased compounds from lignocellulose. This constructed hybrid process could be used to valorize lignocellulose to FOL by sequential catalysis with biochar Sn-CRS chemocatalyst and FF182 cell biocatalyst in the presence of formic acid.…”

A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum

“…The fed-batch reaction was carried out in optimal conditions as described above. The initial reaction mixture (0.5 mL) containing 5 g/L Reb A, 12.5 g/L UDPG, 50 mM PBS (pH 8.0), and 72 OD 600 (27.36 g DCW/L) StUGT cell catalysts was incubated at 30°C for 60 h. In the fed-batch reaction, 5 g/L RA was added once at 16 h; in another reaction, 2.5 g/L Reb A was added twice at 16 and 30 h. Samples were collected at different time intervals (16,30,36,42,48,52, and 60 h) to measure Reb A and Reb D concentrations.…”