Efficient production of NV colour centres in nanodiamonds using high-energy electron irradiation

Dantelle, Géraldine; Slablab, Abdallah; Rondin, Loïc; Lainé, Frédéric; Carrel, Frédéric; Bergonzo, P.; Perruchas, Sandrine; Gacoin, Thierry; Treussart, François; Roch, Jean-François

doi:10.1016/j.jlumin.2009.12.003

Cited by 49 publications

(47 citation statements)

References 12 publications

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“…Preparation of the ND sample was achieved following a procedure reported previously [9,37]. Commercial HPHT diamond nanocrystals are first irradiated using high-energy electrons, then annealed at 800…”

Section: Experimental Methodsmentioning

confidence: 99%

Photophysics of single nitrogen-vacancy centers in diamond nanocrystals

et al. 2015

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A study of the photophysical properties of nitrogen-vacancy (NV) color centers in diamond nanocrystals of size 50 nm or below is carried out by means of second-order time-intensity photon correlation and cross-correlation measurements as a function of the excitation power for both pure charge states, neutral and negatively charged, as well as for the photochromic state, where the center switches between both states at any power. A dedicated three-level model implying a shelving level is developed to extract the relevant photophysical parameters coupling all three levels. Our analysis confirms the very existence of the shelving level for the neutral NV center. It is found that it plays a negligible role on the photophysics of this center, whereas it is responsible for an increasing photon bunching behavior of the negative NV center with increasing power. From the photophysical parameters, we infer a quantum efficiency for both centers, showing that it remains close to unity for the neutral center over the entire power range, whereas it drops with increasing power from near unity to approximately 0.5 for the negative center. The photophysics of the photochromic center reveals a rich phenomenology that is to a large extent dominated by that of the negative state, in agreement with the excess charge release of the negative center being much slower than the photon emission process.

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Section: Experimental Methodsmentioning

confidence: 99%

Photophysics of single nitrogen-vacancy centers in diamond nanocrystals

et al. 2015

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“…The fNDs were prepared as described by Dantelle et al [14]. Briefly, high-pressure high-temperature (HPHT) nanodiamonds, purchased from Van Moppe, were irradiated with a 13.9 MeV electron beam for 1 hour, to create vacancies.…”

Section: Methodsmentioning

confidence: 99%

“…The fluorescence of fNDs arises from the embedded nitrogen-vacancy (NV) color centers that are created in high-pressure high-temperature type 1b synthetic diamonds, with emission in the red and near infrared regions of the spectrum [14]–[16]. NV color centers do not photobleach or blink.…”

Section: Introductionmentioning

confidence: 99%

Influence of the Internalization Pathway on the Efficacy of siRNA Delivery by Cationic Fluorescent Nanodiamonds in the Ewing Sarcoma Cell Model

et al. 2012

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Small interfering RNAs (siRNAs) are powerful tools commonly used for the specific inhibition of gene expression. However, vectorization is required to facilitate cell penetration and to prevent siRNA degradation by nucleases. We have shown that diamond nanocrystals coated with cationic polymer can be used to carry siRNAs into Ewing sarcoma cells, in which they remain traceable over long periods, due to their intrinsic stable fluorescence. We tested two cationic polymers, polyallylamine and polyethylenimine. The release of siRNA, accompanied by Ewing sarcoma EWS-Fli1 oncogene silencing, was observed only with polyethylenimine. We investigated cell penetration and found that the underlying mechanisms accounted for these differences in behavior. Using drugs selectively inhibiting particular pathways and a combination of fluorescence and electronic microscopy, we showed that siRNA gene silencing occurred only if the siRNA:cationic nanodiamond complex followed the macropinocytosis route. These results have potential implications for the design of efficient drug-delivery vectors.

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“…Fluorescent nanodiamonds (FNDs) are one of the new classes of fluorescent probes with high potential to be very successful in practical imaging applications [7–17]. Fluorescent properties of FNDs are the result of point defects embedded in the crystal lattice.…”

Section: Introductionmentioning

confidence: 99%

FRET Enhanced Fluorescent Nanodiamonds

Fudala¹,

Raut²,

Maliwal³

et al. 2014

CPB

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Fluorescent nanodiamonds (FNDs) are one of the new and very promising biocompatible nanomaterials that can be used both as fluorescence imaging agent and a highly versatile platform for controlled functionalization to target and deliver a wide spectrum of therapeutic agents. Among the remarkable fluorescence properties are excellent photo-stability, emission between 600–700nm, quantum yield of 1 and moderately long fluorescence lifetimes. However the low absorption cross section of fluorescent (N-V)− centers limits FNDs' brightness. In this work we show that an approach based on the Forster resonance energy transfer (FRET) may significantly enhance the fluorescence signal observed from a single ND. We demonstrate that organic dyes (fluorophores) attached to the FND surface can efficiently transfer the excitation energy to (N-V)− centers. Multiple dyes positioned in close proximity to the ND facile surface may serve as harvesting antennas transferring excitation energy to the fluorescent centers. We propose that, with the help of some of the functional groups present on the FND surface, we can either directly link flurophores or use scalable dendrimer chemistry to position many organic dyes at a calibrated distance. Also, the remaining multiple functional groups will be still available for particle targeting and drug delivery. This opens a new way for designing a new type of theranostics particles of ultra-high brightness, high photostability, specific targeting, and high capacity for drug delivery.

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Efficient production of NV colour centres in nanodiamonds using high-energy electron irradiation

Cited by 49 publications

References 12 publications

Photophysics of single nitrogen-vacancy centers in diamond nanocrystals

Photophysics of single nitrogen-vacancy centers in diamond nanocrystals

Influence of the Internalization Pathway on the Efficacy of siRNA Delivery by Cationic Fluorescent Nanodiamonds in the Ewing Sarcoma Cell Model

FRET Enhanced Fluorescent Nanodiamonds

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