Efficient production of polymalic acid from xylose mother liquor, an environmental waste from the xylitol industry, by a T-DNA-based mutant of Aureobasidium pullulans

Feng, Jun; Li, Tianfu; Zhang, Xiao; Chen, Jie; Zhao, Tiantao; Zou, Xiang

doi:10.1007/s00253-019-09974-x

Cited by 24 publications

(6 citation statements)

References 40 publications

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“…Besides, for the two fermentation systems, there have a significant changed in the relative abundances of Aureobasidium pullulans and Cladosporium delicatulum during fermentation process. Previous studies indicated that Aureobasidium pullulans was related to the submerge fermentation at industrial scale …”

Section: Resultsmentioning

confidence: 99%

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Enantiomeric effect of paclobutrazol on the microorganism composition during wine fermentation

et al. 2020

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Pesticide residues in food can bring potential risks to human health and has been widely concerned in recent years. In the current study, the influence of paclobutrazol, which resided in raw material (grape) on wine fermentation process, were investigated. The degradation kinetic results indicated that the enantiomers of paclobutrazol not be degraded during 30 days of fermentation process. In order to achieve the fermented microorganism information of diversity, community composition, and function, the analysis of 16S rRNA and ITS sequencing were performed. Results demonstrated that the dominant microorganisms multiplied and the microbial diversity in the samples decreased as the fermentation process progresses. Furthermore, the paclobutrazol stimulated the growth of Pichia, which was observed during wine fermentation and which may have an underlying impact on the quality of the wine. The above results inferred that paclobutrazol residue could disturb the microbial community stability during wine fermentation, and the stable existence of paclobutrazol will cause potential risks to food safety and human health. In this work, we have successfully devised a method to investigate the influences of pesticide residues in raw materials during food processing and conclusions from this study could provide basis for dietary risk assessment.

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Section: Resultsmentioning

confidence: 99%

“…Previous studies indicated that Aureobasidium pullulans was related to the submerge fermentation at industrial scale. 33,34…”

Section: F I G U R E 4 Fungi Composition Of Wine Samples In Genus Levelmentioning

confidence: 99%

Enantiomeric effect of paclobutrazol on the microorganism composition during wine fermentation

et al. 2020

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“…The pBARGPE1 plasmid with the strong promoter of Aspergillus nidulans P gpdA was used for gene overexpression, and the pK2 series shuttle plasmid was used to integrate the DNA overexpression cassette into the genome, as described in our previous work. 29 Total RNA was extracted using a fungal RNA kit (Omega, USA). HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, China) was used to transcribe RNA into total cDNA, and the cDNA library was used as a template to amplify the target gene.…”

Section: Methodsmentioning

confidence: 99%

Carbon-economic biosynthesis of poly-2-hydrobutanedioic acid driven by nonfermentable substrate ethanol

Wang

et al. 2022

Green Chem.

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“…A diversity of saccharides (e.g., glucose, sucrose, fructose, and xylose) can be utilized by A. pullulans as a carbon source for metabolism in the cytoplasm and mitochondrial matrix, producing malate for PMLA synthesis; in contrast, P. polycephalum can only utilize d -glucose [ 62 – 65 ]. Genomic sequences revealed that A. pullulans varieties encode a large number of enzymes responsible for various sugar degradation or transportation, including polysaccharide lyase, glycosyltransferase, glycoside hydrolase, carbohydrate esterase, and carbohydrate-binding modules [ 66 ].…”

Section: Bioproduction Of Pmla and Its Characterizationsmentioning

confidence: 99%

Polymalic acid for translational nanomedicine

Huang

Qian

et al. 2022

J Nanobiotechnol

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With rich carboxyl groups in the side chain, biodegradable polymalic acid (PMLA) is an ideal delivery platform for multifunctional purposes, including imaging diagnosis and targeting therapy. This polymeric material can be obtained via chemical synthesis, or biological production where L-malic acids are polymerized in the presence of PMLA synthetase inside a variety of microorganisms. Fermentative methods have been employed to produce PMLAs from biological sources, and analytical assessments have been established to characterize this natural biopolymer. Further functionalized, PMLA serves as a versatile carrier of pharmaceutically active molecules at nano scale. In this review, we first delineate biosynthesis of PMLA in different microorganisms and compare with its chemical synthesis. We then introduce the biodegradation mechanism PMLA, its upscaled bioproduction together with characterization. After discussing advantages and disadvantages of PMLA as a suitable delivery carrier, and strategies used to functionalize PMLA for disease diagnosis and therapy, we finally summarize the current challenges in the biomedical applications of PMLA and envisage the future role of PMLA in clinical nanomedicine. Graphical Abstract

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Efficient production of polymalic acid from xylose mother liquor, an environmental waste from the xylitol industry, by a T-DNA-based mutant of Aureobasidium pullulans

Cited by 24 publications

References 40 publications

Enantiomeric effect of paclobutrazol on the microorganism composition during wine fermentation

Enantiomeric effect of paclobutrazol on the microorganism composition during wine fermentation

Carbon-economic biosynthesis of poly-2-hydrobutanedioic acid driven by nonfermentable substrate ethanol

Polymalic acid for translational nanomedicine

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