Efficient production of polysaccharide by Chaetomium globosum CGMCC 6882 through co-culture with host plant Gynostemma pentaphyllum

Wang, Zichao; Gao, Wenshuo; Li, Xiaoying; Chen, Peizhang; Lu, Wenbo; Wang, Fuzhuan; Li, Haifeng; Sun, Qi; Zhang, Huiru

doi:10.1007/s00449-019-02169-8

Cited by 7 publications

(1 citation statement)

References 34 publications

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“…The total saponins content determined using the method of vanillin colorimetry was up to 83.0% in TSGP, indicating that the non-saponin compounds occupied 17.0%, which was coincident with previous studies regarding that G. pentaphyllum contained saponins, flavonoids, polysaccharide, sterol and other chemical compositions. [38][39][40] In this regard, the 17.0% non-saponin compounds derived from nonpolar D101 macroporous resin column might be sterol, flavonoids and other chemical ingredients, [38][39][40] but the significantly lower concentration of flavonoids and sterols in TSGP was possibly insufficient to show biological activities, relative the saponins-enriched fraction, although sterol and flavonoids was approved to exert the pro- tective effects against atherosclerosis and other benefits. 40,41) Furthermore, antioxidant activities of TSGP were verified by DPPH , O 2 − , HO and ferric reducing ability of plasma, indicating that TSGP had a significant antioxidant function (Fig.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Saponins-Enriched Fraction of <i>Gynostemma pentaphyllum</i> against High Choline-Induced Vascular Endothelial Dysfunction and Hepatic Damage in Mice

Yang

Zhao

Ren

et al. 2020

Biological & Pharmaceutical Bulletin

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Choline as a quaternary amine nutrient is metabolized to trimethylamine by gut microbiota and subsequently oxidized to circulating trimethylamine-N-oxide (TMAO), a gut-derived metabolite associated with liver toxicity and cardiovascular disease. The study was to probe the possible vasoprotective and hepatoprotective effects of total saponins of Gynostemma pentaphyllum (TSGP) in 3% high-choline water-feeding mice. The purified TSGP was obtained with content of 83.0% saponins, and its antioxidant activities were evaluated in vitro. Furthermore, the mice fed with high choline for 8 weeks significantly expressed vascular endothelial dysfunction and liver oxidative stress (p < 0.01 vs. Normal). Administration of TSGP at 400 and 800 mg/kg•body weight (b.w.) significantly lowered the serum total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), endothelin-1 (ET-1) and thromboxane A 2 (TXA 2) levels, as well as hepatic malondialdehyde (MDA) formation, but effectively elevated the serum nitric oxide (NO), endothelial nitric oxide synthase (eNOS) and prostaglandin I 2 (PGI 2) levels, as well as alanine aminotransferase (ALT), aspartate aminotransferase (AST), T-superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in high choline-fed mice. Hematoxylin-eosin (H&E) and oil red O staining also suggested that TSGP could exert the significant protection against endothelial dysfunction and liver injury in high choline-treated mice. These findings suggest that TSGP is of the saponins-enriched extract, and is a good candidate of dietary supplement and therapeutic application in vascular and hepatic oxidative injury.

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