Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli

Abstract

Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20±0.02mol/mol) than glucose (0.48±0.03mol/mol). The concentration and molar yield of succinic acid were 22.40±0.12g/L and 1.13±0.02mol/mol total sugar respectively after 72h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57±0.17g/L) and galactose (18.03±0.10g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production.

Effective production of succinic acid from coconut water (Cocos nucifera) by metabolically engineered Escherichia coli with overexpression of Bacillus subtilis pyruvate carboxylase

“…Fermentation of C. nucifera water with E. coli M6PM produced a final concentration of 11.78 ± 0.02 g/L succinic acid and yield of 1.23 ± 0.01 mol/mol, 0.66 ± 0.01 g/g total sugars after 72 h dual-phase fermentation in M9 medium. This makes up 72% of the maximum theoretical yield and is comparable to some results of succinic acid production in the literature [[38], [39], [40], [41], [42]]. Hence , C. nucifera water could be used for the production of succinic acid in large scale and other biochemicals.…”

Demonstration of parallel algal processing: production of renewable diesel blendstock and a high-value chemical intermediate

“…Previous A. succinogenes 130Z batch fermentations of macroalgae achieved productivities of 0.3-3.9 g L −1 h −1 and yields of 0.73-0.92 g g −1 total sugars. [13][14][15] It must be noted that our titer values are a function of the sugar concentration in the PAL and so comparisons with other sugar sources are not necessarily applicable. Our yields and productivity values are lower than those seen with other sugar sources and we attribute that to the higher salt concentration introduced during the pretreatment and neutralization steps.…”

“…13,14 Similarly, sugars released from Palmaria palmata using a two-step hydrochloric acid followed by enzymatic hydrolysis pretreatment were fermented to produce succinic acid by an engineered E. coli strain that achieved a maximum titer, yield, and productivity of 22.4 g L −1 , 0.73 g g −1 total sugars, and 0.3 g L −1 h −1 respectively. 15 Recently, combined algal processing (CAP) demonstrated integrated biofuel production from pretreated algal biomass. 16 The CAP process employs acid pretreatment of algal biomass to provide whole-slurry for ethanol fermentation followed by distillation and lipid extraction of the stillage to generate biofuel precursors.…”

Complete Genome Sequence of Actinobacillus succinogenes GXAS137, a Highly Efficient Producer of Succinic Acid

“…SA production has been accomplished by different microorganisms, including Anaerobiospirillum succiniciproducens , Actinobacillus succinogenes , Basfia succiniciproducens , Mannheimia succiniciproducens ( 6 ), Saccharomyces cerevisiae ( 7 ), and Escherichia coli ( 8 ). Although these organisms have achieved a competitive performance, A. succinogenes , a capnophilic anaerobic Gram-negative bacterium, is promising because of its ability to form SA naturally at appreciable yields and productivities from a broad range of carbon sources ( 9 – 11 ).…”