Efficient production of α-monoglucosyl hesperidin by cyclodextrin glucanotransferase from Bacillus subtilis

Shi, Yuan; Fang, Jingyi; Gan, Tian; Lu, Yuele; Zhu, Linjiang; Chen, Xiaolong

doi:10.1007/s00253-023-12628-8

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Protein secretion and expression not only enhance degradation efficiency but also streamline the purification process and conserve resources, making it a widely utilized technical approach. − Bacillus subtilis is one of the most extensively studied gene expression systems due to its robust protein secretion, well-defined genetic background, preference for codon-free sequences, rapid growth, and food and drug administration approval as a safe organism. − The selection of the appropriate promoter and optimal signal peptides (SPs) for protein secretion significantly influences the engineering of B. subtilis for efficient production and extracellular secretion of heterologous proteins. , Promoters, as key regulatory elements, directly govern alloprotein expression levels.…”

Section: Introductionmentioning

confidence: 99%

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

Zhao,

Wang,

Tian

et al. 2024

J. Agric. Food Chem.

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The residues of acifluorfen present a serious threat to the agricultural environment and sensitive crops. DnrA, a nitroreductase, is an intracellular enzyme that restricts the application of wild-type Bacillus sp. Za in environmental remediation. In this study, two strategies were employed to successfully secrete DnrA in strains SCK6 and Za, and the secretion expression conditions were optimized to achieve rapid degradation of acifluorfen. Under the optimal conditions, the relative activities of the DnrA supernatant from strains SCK6-D and Za-W were 3.06-fold and 3.53-fold higher than that of strain Za, respectively. While all three strains exhibited similar tolerance to different concentrations of acifluorfen, strains SCK6-D and Za-W demonstrated significantly faster degradation efficiency compared to strain Za. Furthermore, the DnrA supernatant from strains SCK6-D and Za-W could effectively reduce the toxicity of acifluorfen on maize and cucumber seedlings. This study provides an effective technical approach for the rapid degradation of acifluorfen.

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Section: Introductionmentioning

confidence: 99%

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

Zhao,

Wang,

Tian

et al. 2024

J. Agric. Food Chem.

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“…Moreover, it has been studied as an anti-obesity [17], neuroprotective [18,19], anti-cancer [20], and anti-aging agent [21,22]. In the food industry, hesperidin derivatives are commonly used as food additives to enhance the antioxidant and antimicrobial activities of active packaging films instead of hesperidin, given its limited water solubility, bitterness, and poor oral bioavailability [23,24]. Even though hesperidin has been highly researched for its potential therapeutic and environmental applications, its extraction from different citrus matrices has proven to be a challenge.…”

Section: Introductionmentioning

confidence: 99%

A Two-Step Approach to Orange Peel Waste Valorization: Consecutive Extraction of Pectin and Hesperidin

Figueira,

Pereira,

Castilho

2023

Foods

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Citrus consumption translates into large amounts of residue, the disposal of which is associated with environmental issues and high costs. Current trends in citrus waste focus on the extraction of highly valued bioactive compounds via single-compound extraction. There is a lack of knowledge on how these methodologies can be introduced into extraction schemes of bioactive compounds, maximizing the residue potential and reducing its amount. The present work aimed to address this issue by designing a consecutive extraction of pectin and hesperidin from orange peel waste. A novel method for extraction and precipitation of hesperidin with an eco-friendly approach is also presented. After neutral pretreatment, pectin extraction was conducted under acidic conditions, followed by hesperidin extraction with a drastic pH change. Pectin had a high AUA content (66.20 ± 1.25%), meeting the criteria for use in the food industry. The best-tested conditions for hesperidin extraction (30 min, 70 °C, 1:10 (w/v)) provided a yield of 1% and a purity of 84%. The designed extraction scheme shows the potential of citrus waste as a source of bioactive compounds of good quality and high interest in the food industry while following the principles of green chemistry and circular economy.

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Whole-cell biocatalysis for ε-poly-l-lysine production by a food-grade recombinant Bacillus subtilis

Li,

Guo,

Sun

et al. 2024

Enzyme and Microbial Technology

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Efficient production of α-monoglucosyl hesperidin by cyclodextrin glucanotransferase from Bacillus subtilis

Cited by 6 publications

References 41 publications

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

Nitroreductase DnrA, Utilizing Strategies Secreted in Bacillus sp. Za and SCK6, Enhances the Detoxification of Acifluorfen

A Two-Step Approach to Orange Peel Waste Valorization: Consecutive Extraction of Pectin and Hesperidin

Whole-cell biocatalysis for ε-poly-l-lysine production by a food-grade recombinant Bacillus subtilis

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