Efficient propagation and rooting of three citrus rootstocks using different plant growth regulators

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

“…In most of the rootstocks, maximum rooting percentage was obtained in the presence of 2 ppm NAA (Table 3). This finding is in agreement with different researchers (Edriss and Burger, 1984;Tallón et al, 2012;Gill et al, 1995), stating that, in various Citrus species, 2 ppm of NAA was the optimum concentration for maximum rooting percentage. In addition, other studies have shown that maximum rooting, in various Citrus species, can be obtained in lower NAA concentrations, such as 1 ppm (Kitto and Young, 1981;Bordon et al, 2000;Al Bahrany, 2002;Rathore et al, 2007) or 0.5 ppm of NAA (Savita Singh et al, 2011).…”

“…Al Bahrany (2002) observed that the number of roots per explant in Citrus aurantifolia 'Swing' is increased with the simultaneous increase of NAA in the media, and 2 ppm of NAA was the optimum concentration for maximum number of roots per explant. Tallón et al (2012), also observed in Sour Orange that maximum roots per explant were obtained with 2 ppm of NAA. Furthermore, in Cleopatra (Tallón et al, 2012), Citrus macrophylla (Ghorbel et al, 1998;Bordon et al, 2000) and Citrus reticulata 'Blanco' (Gill et al, 1995) 1 ppm of NAA was the optimum concentration of auxin for maximum number of roots per explants.…”

“…Tallón et al (2012), also observed in Sour Orange that maximum roots per explant were obtained with 2 ppm of NAA. Furthermore, in Cleopatra (Tallón et al, 2012), Citrus macrophylla (Ghorbel et al, 1998;Bordon et al, 2000) and Citrus reticulata 'Blanco' (Gill et al, 1995) 1 ppm of NAA was the optimum concentration of auxin for maximum number of roots per explants.…”

“…Shoot length was not significantly affected by the presence of BA in all rootstocks studied, except Volkameriana, in which significant differences were shown among the different concentrations of BA, and 'Rubidoux', in which the presence of BA significantly decreased shoot length. Tallón et al (2012), observed that the presence of BA did not affect significantly the shoot length in sour orange, but significantly affected the shoot length in Alemow and Cleopatra rootstocks. Perez-Tornero et al (2010), in various lemon varieties, and Savita Singh et al (2011) in Citrus jambhiri 'Lush', noted that BA presence significantly increased shoot length.…”

“…Highest proliferation percentage for most of the rootstocks was observed at a concentration of 2 ppm BA. Tallón et al (2012), reported also in Citrus rootstocks 'Alemow' and 'Cleopatra' the highest shoot proliferation using 2 ppm of BA. Rootstocks Citrange 'Carrizo' and Volkameriana also presented 100% shoot induction in 1 ppm of BA (Table 2).…”