Efficient protocol for in vitro propagation of Laelia rubescens Lindl. from asymbiotic seed germination

Mayo-Mosqueda, Alberto; Maceda-López, Luis F.; Andrade‐Canto, Silvia B.; Noguera-Savelli, Eliana; Caamal-Velázquez, Humberto; Cano-Sosa, Julia; Alatorre‐Cobos, Fulgencio

doi:10.1016/j.sajb.2020.07.030

Cited by 8 publications

(3 citation statements)

References 44 publications

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“…A report by Mayo-Mosqueda [47] suggested that the capsule age and maturity influenced orchid seed viability. Embryos would only be viable after the ripening of the capsules [41].…”

Section: Discussionmentioning

confidence: 99%

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

et al. 2023

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Paraphalaenopsis labukensis Shim, A. Lamb & C.L. Chan (P. labukensis) is an endangered monopodial epiphytic orchid threatened due to habitat fragmentation and overharvesting. Consequently, this research aimed to achieve in vitro propagation of P. labukensis through asymbiotic seed germination and seedling development. This study focused on identification and optimizing capsule maturity, basal media and nutrient requirements. In addition, after hand pollinating the plant, their capsules’ flowers, length, girth, and colour variations were recorded weekly to ascertain morphological maturity characters of this species. Murashige and Skoog (MS), Knudson C (KC), and Vaccin and Went (VW) basal media were used to determine seed vegetation at 60, 90, and 120 days. Subsequently, for the proliferation and development of protocorms, the best basal media were those supplemented with different organic additives (coconut water (CW) and banana homogenate (BH)) and plant growth regulators (PGRs). As a result, 120 days after pollination (DAP) was chosen as the best capsule age for germination since the capsules had reached maturity. The highest germination rate was attained after 90 days of culture in Knudson C (KC) medium, with 98.78 ± 0.89%, followed by Murashige and Skoog (MS) medium, with 92.80 ± 3.26%. The KC supplemented with 0.5 mg/L NAA media documented a maximum percentage of 17.25 ± 0.96%. The 5 g/L (w/v) BH recorded a higher protocorm proliferation percentage than CW. Additionally, after 150 days of culture (DOC), this medium combination produced the most leaves, averaging four or five. The result of this present study has successfully established an effective in vitro propagation protocol for this species.

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“…A report by Mayo-Mosqueda [47] suggested that the capsule age and maturity influenced orchid seed viability. Embryos would only be viable after the ripening of the capsules [41].…”

Section: Discussionmentioning

confidence: 99%

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

et al. 2023

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“…One technique to study seed germination includes in vitro cultivation, the most developed micropropagation technique for quickly and successfully obtaining orchid seedlings, mainly with threatened species or of commercial interest (Mayo-Mosqueda et al 2010, Salazar-Mercado 2012, Aguilar-Morales & López-Escamilla 2013. Mayo-Mosqueda et al (2020), developed this technique with L. speciosa (subject to special protection under Mexican law) obtaining 100 % germination, showing that in vitro micropropagation has high potential in the quest to reduce the impact of plant subtraction and maintain genetic diversity as a supporting ES.…”

Section: Botanical Sciences / Online Firstmentioning

confidence: 99%

Ecosystem services provided by orchids: a global analysis

Hernández-Mejía,

De la Rosa-Manzano,

Delgado-Sánchez

2024

Bot. Sci.

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Background: Orchids constitute one of the most diverse group of plants, they provide a large number of benefits to human society. Questions: What are the ecosystem services provided by orchids? Data description: We performed a systematic review based on 413 articles, of which 224 provide relevant information, focusing on publications by ecosystem service category, continent, genus, and quartile of journal. Study site and years studied: studies of orchids on all continents from 2000 to 2021. Methods: databases reviewed were BioOne, CONRICyT, EBSCO, EndNote, Google Scholar, JSTOR, Mendeley, ScienceDirect, Wiley library, as well as reviews of specialized books. Results: We found 75 articles on cultural services, followed by 66 on provisioning services, 64 on supporting and 19 on regulating services. The Epidendroideae subfamily was the most mentioned with 90.9 %, with the genus Dendrobium having the most mentions (39.13 %). Most of the orchid ecosystem service research was conducted on the American and Asian continents, where cultural and provisioning services were prevalent, respectively. Of all the publications, 54.7 % belong to quartile 1, and 2.4 % to quartile 4. Conclusions: This research contributes to our understanding of ecosystem services provided by orchids, which may allow us a more comprehensive understanding of how human society benefits from orchids, and what could be lost if these species are not conserved and used sustainably.

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“…In vitro planting is the process of germinating a seed embryo in a laboratory under favourable growth circumstances similar to that of a biological organism. Seed germination performance in vitro is affected by a variety of factors, including culture conditions, seed maturity, carbohydrates, plant growth regulators, and organic additions [35,36]. T. foenum-graecum includes several key phytochemicals that give birth to numerous therapeutic characteristics required to treat a variety of human health conditions.…”

Section: Journal Of Asian Scientific Researchmentioning

confidence: 99%

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

Agha

Sidik

Radzun

et al. 2022

5003

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Trigonella foenum-graecum is an important leguminous plant used in medicine and human health since ancient times, and the various civilizations of the world, as the Egyptian, Greek, and Indian civilizations, have been used in traditional medicine. Trigonella foenum-graecum contains a wide range of pharmacological effects because of the presence of several beneficial plant secondary metabolites. However, the resistant nature of this plant causes major challenges in in vitro multiplication, emphasizing the need to assess novel approaches for the propagation of this plant. As a result, this study reports on the effects of BAP, NAA, and 2,4-D on T. foenum graecum seed germination in vitro. The germination percentage recorded every week for 4 weeks, the parameter's last mean was calculated. The results showed that 2.0 mg/L BAP created the best effect in the parameter studied. The results revealed that 2.0 mg/l BAP provided the greatest response germination percentage (86 ±2.357%). These results highlight the optimum value of growth hormones producing T. foenum graecum plant with a minimum germination rate in a large scale.

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Efficient protocol for in vitro propagation of Laelia rubescens Lindl. from asymbiotic seed germination

Cited by 8 publications

References 44 publications

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

Ecosystem services provided by orchids: a global analysis

The Influence of Different Concentrations of Plant Hormones in Vitro on Seeds Germination of Fenugreek (Trigonella Foenum-Graecum)

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