Efficient repair of DNA double strand breaks in individuals from high level natural radiation areas of Kerala coast, south-west India

Jain, Vinay; Saini, Divyalakshmi; Kumar, Pradeep; Jaikrishan, G.; Das, Birajalaxmi

doi:10.1016/j.mrfmmm.2017.09.003

Cited by 20 publications

(21 citation statements)

References 68 publications

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“…The spontaneous level of DNA double strand breaks (DSBs) has not shown any increase in DSBs, rather showed marginal reduction in HLNRA individuals belonging to background dose group >5mGy/year [ 18 ]. Interestingly, DNA damage and repair study in peripheral blood mono-nuclear cells (PBMC) using gamma H2AX marker and comet assay revealed that individuals from HLNRA groups (>5.0mGy/year) have better repair efficiency as compared to NLNRA group [ 21 , 23 ]. Our group has also shown in vivo radio-adaptive response among HLNRA group of individuals after a challenge dose of 1.0 and 2.0Gy of gamma radiation[ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Global transcriptome profile reveals abundance of DNA damage response and repair genes in individuals from high level natural radiation areas of Kerala coast

Jain

Das

2017

PLoS ONE

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The high level natural radiation areas (HLNRA) of Kerala coast in south west India is unique for its wide variation in the background radiation dose (<1.0mGy to 45mGy/year) and vast population size. Several biological studies conducted in this area did not reveal any adverse effects of chronic low dose and low dose rate radiation on human population. In the present study, global transcriptome analysis was carried out in peripheral blood mono-nuclear cells of 36 individuals belonging to different background dose groups [NLNRA, (Group I, ≤1.50 mGy/year) and three groups of HLNRA; Group II, 1.51–5.0 mGy/year), Group III, 5.01-15mGy/year and Group IV, >15.0 mGy/year] to find out differentially expressed genes and their biological significance in response to chronic low dose radiation exposure. Our results revealed a dose dependent increase in the number of differentially expressed genes with respect to different background dose levels. Gene ontology analysis revealed majority of these differentially expressed genes are involved in DNA damage response (DDR) signaling, DNA repair, cell cycle arrest, apoptosis, histone/chromatin modification and immune response. In the present study, 64 background dose responsive genes have been identified as possible chronic low dose radiation signatures. Validation of 30 differentially expressed genes was carried out using fluorescent based universal probe library. Abundance of DDR and DNA repair genes along with pathways such as MAPK, p53 and JNK in higher background dose groups (> 5.0mGy/year) indicated a possible threshold dose for DDR signaling and are plausible reason of observing in vivo radio-adaptive response and non-carcinogenesis in HLNRA population. To our knowledge, this is the first study on molecular effect of chronic low dose radiation exposure on human population from high background radiation areas at transcriptome level using high throughput approach. These findings have tremendous implications in understanding low dose radiation biology especially, the effect of low dose radiation exposure in humans.

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Section: Introductionmentioning

confidence: 99%

Global transcriptome profile reveals abundance of DNA damage response and repair genes in individuals from high level natural radiation areas of Kerala coast

Jain

Das

2017

PLoS ONE

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“…This indicates that DNA DSB induction by chronic LDR radiation exposure might be detected with γ-H2AX. In recent years, it has been reported that DNA DSB levels in inhabitants of high natural background radiation (HNBR) area can be detected by the γ-H2AX assay [43][44][45]. Although there was no significant difference, PBL derived from the HNBR population had trend to higher DNA DSB levels compared to PBLs derived from residents in other areas.…”

Section: Perspective For Future Biodosimetrymentioning

confidence: 95%

“…Although there was no significant difference, PBL derived from the HNBR population had trend to higher DNA DSB levels compared to PBLs derived from residents in other areas. Interestingly, DNA damage repair capacity in PBLs derived from the HNBR population was elevated, suggesting the existence of adaptive response by chronic LDR radiation [43,45]. Taken together, monitoring of DNA DSBs by γ-H2AX may not be suitable for the assessment of cumulative dose of chronic LDR radiation, but is very valuable for evaluating biological effects of chronic LDR radiation exposure.…”

Section: Perspective For Future Biodosimetrymentioning

confidence: 99%

Assessment of DNA Damage Induction in Farm Animals After the FNPP Accident

Nakamura

2019

Low-Dose Radiation Effects on Animals and Ecosystems

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Since the Fukushima Daiichi Nuclear Power Plant (FNPP) accident is a radiation accident which occurred as the aftermath of a devastating natural disaster, the Great East Japan Earthquake, people had to face radiation risks in the chaotic state. In the situation where general citizens, scientists and politicians were all confused, the importance of accurately assessing biological risks of radiation emerged. To understand the biological effects of radiation exposure by the accident, we measured the DNA damage level using the DNA double-strand break (DSB) marker phosphorylated histone H2AX in peripheral blood lymphocytes from farm animals left behind within a 20-km radius from FNPP (the ex-evacuation zone). As a result, statistically higher levels of DNA DSBs were detected from cattle in the exevacuation zone compared to non-affected control; however, it was not able to accurately evaluate the radiation dose from this accident with phosphorylated H2AX. This is thought to be caused by the fact that various changes of metabolism with the lapse of time and the living environment of individual organisms occurred. It may, therefore, be difficult to evaluate the exposure dose of chronic low-dose-rate (LDR) radiation by a single biomarker. However, in the inevitable modern society of radiation exposure and fear of nuclear accidents, our results showed that trying a certain dosimetric biomarker for the assessment of biological impacts of long-term LDR radiation exposure is effective and crucial for the protection from radiation.

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“…Majority of oxidative damages include oxidative clustered DNA lesions (OCDLs) [7].Understanding the risk from the natural background radiation, Vinay Jain et al, the authors studied the repair of DNA double strand breaks (DSBs)in high background radiation area and determined the low dose as 1.51-5.0 mGy/year and high dose as >5.0 mGy/year. The results demonstrated that faster repair of DSBs and involvement of nonhomologous end joining repair pathway in High level natural radiation area group of individuals was assessed as adaptive response [8]. However, further studies on DNA damage induction and repair kinetics are required for the strong evidence [9,10].…”

mentioning

confidence: 94%

“…We identified 13 papers that fulfilled the criteria of natural background radiation impact on the health over the terrestrial radiation, intakes of naturally occurring radionuclides through inhalation and ingestion [5-7, 12, 13, 16, 17, 19, 21-23, 27, 28]. There are several molecular epidemiological studies that revealed low dose of natural background radiation beneficial for health, as it induce the adaptive response in the DNA damage repair capacity and antioxidant capacity of inhabitants in the high background-radiation area [1-3, 9-11, 15, 18, 25].There were just four papers, which suspected the adverse effect from natural background radiation on the human health [4,8,11,14,18,20]. However, the authors still recommended studying this question, as to be sure that radiation is a dominant risk factor for the health.…”

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confidence: 99%

The Impact of the Background Radiation on the Health

Туруспекова¹,

Tsoy²

2019

OF THE NATIONAL ACADEMY OF SCIENCES OF THE REPUBLIC OF KAZAKHST

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Abzhanov Arkhat, prof. (Boston, USA), Abelev S.K., prof. (Moscow, Russia), Aitkhozhina N.А., prof., academician (Kazakhstan) Akshulakov S.K., prof., academician (Kazakhstan) Alchinbayev М.K., prof., academician (Kazakhstan) Batpenov N.D., prof., corr. member (Kazakhstan) Berezin V.Ye., prof., corr. member. (Kazakhstan) Bersimbayev R.I., prof., academician (Kazakhstan) Berkinbaev S.F., prof. (Kazakhstan) Bisenbayev А.K., prof., academician (Kazakhstan) Bishimbayeva N.K., prof., academician (Kazakhstan) Botabekova Т.K., prof., corr. member. (Kazakhstan) Bosch Ernesto, prof. (Spain) Davletov Kairat, PhD, associate professor, executive Secretary Dzhansugurova L.B., Cand. biol., prof. (Kazakhstan) Ellenbogen Adrian, prof. (Tel-Aviv, Israel), Zhambakin K.Zh., prof., academician (Kazakhstan), deputy editor-in-chief Ishchenko Alexander, prof. (Villejuif, France) Isayeva R.B., prof. (Kazakhstan) Kaydarova D.R., prof., academician (Kazakhstan) Kokhmetova A., prof., corr. member (Kazakhstan) Kuzdenbayeva R.S., prof., academician (Kazakhstan) Lokshin V.N., prof., corr. member (Kazakhstan) Los D.А., prof. (Moscow, Russia) Lunenfeld Bruno, prof. (Israel) Makashev E.K., prof., corr. member (Kazakhstan) Mitalipov Sh.M. (America) Muminov Т.А., prof., academician (Kazakhstan) Ogar N.P., prof., corr. member (Kazakhstan) Omarov R.T., cand. biol., prof. (Kazakhstan) Prodeus A.P., prof. (Russia) Purton Saul, prof. (London, UK) Rakhypbekov Т.K., prof., corr. member. (Kazakhstan) Saparbayev Мurat, prof. (Paris, France) Sarbassov Dos, prof. (Houston, USA) Turysbekov E.K., cand. biol., assoc. prof. (Kazakhstan) Sharmanov A.T., prof. (USA) News of the National Academy of Sciences of the Republic of Kazakhstan. Series of biology and medicine.

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Efficient repair of DNA double strand breaks in individuals from high level natural radiation areas of Kerala coast, south-west India

Cited by 20 publications

References 68 publications

Global transcriptome profile reveals abundance of DNA damage response and repair genes in individuals from high level natural radiation areas of Kerala coast

Global transcriptome profile reveals abundance of DNA damage response and repair genes in individuals from high level natural radiation areas of Kerala coast

Assessment of DNA Damage Induction in Farm Animals After the FNPP Accident

The Impact of the Background Radiation on the Health

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