Efficient replication of caprine arthritis-encephalitis virus in goat granulosa cells

Lamara, Ali; Fieni, F.; Mselli-Lakhal, Laïla; Tainturier, D.; Chebloune, Yahia

doi:10.1016/s0168-1702(01)00347-1

Cited by 36 publications

(20 citation statements)

References 30 publications

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“…MVV can replicate in vitro in a wide variety of ovine cells [30], because the restriction that the virus presents under natural conditions is no longer maintained in vitro [29]. Similarly, CAEV, the caprine counterpart of MVV, can replicate in a variety of cells in vitro [14,15,20,27,28,37] and, significantly, HIV-1 can also productively infect different cell lines in vitro including human OMEC [43].…”

Section: Discussionmentioning

confidence: 99%

“…It has been assumed that tissue macrophages are the only target cells for MVV, but several studies have demonstrated that MVV can infect a broader cell range such as oligodendrocytes, astrocytes, fibroblasts [10], endothelial cells [6] and smooth muscle cells [21], among others. It is known that the epithelial cells from granulosa, goat oviduct and those present in goat milk are highly permissive in vitro to the caprine arthritis and encephalitis virus (CAEV) [14,15,27,28]. Even early embryonic cells from in vivo-produced goat embryos transmit this virus [16].…”

Section: Introductionmentioning

confidence: 99%

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Maedi-visna virus infection of ovine mammary epithelial cells

et al. 2006

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-The aim of this work was to perform a complete study of maedi-visna virus (MVV) infected mammary glands of naturally-infected sheep, and to determine if cells other than macrophages undergo a productive viral infection in this organ. Fifteen seropositive and two seronegative ewes were selected from MVV-infected flocks on the basis of clinical indurative mastitis and three sheep from an MVV-free flock. Within the mammary gland, MVV-positive cells were located by immunohistochemistry in the stroma and the epithelial alveolar barrier, most likely the ovine mammary epithelial cells (OMEC) of the acini. In situ hybridization confirmed these findings. Ultrastructural studies showed the presence of lentivirus-like particles budding off the cell surface in the alveolar barrier and also free in the acinar lumen. The presence of mammary histopathological lesions and MVV together with clear indications of productive infection (demonstration of a cytopathic effect in OMEC cultures and infection of co-cultures) were observed in the 15 seropositive and one of the seronegative sheep from the infected flock. These findings demonstrate that the OMEC were infected in vivo and probably underwent productive infection when studied ex-vivo. The OMEC of MVV-free sheep, which had subsequently been infected in vitro with MVV, also showed productive infection when challenged in vitro, confirming the replication of MVV in OMEC in vitro. The presence of MVV-infected OMEC in the mammary gland from infected animals, the productive infection in these OMEC and the release of lentiviral particles to the acinar lumen may have relevance in the pathogenesis and transmission of MVV infection. maedi-visna / mammary gland / epithelial cells / indurative mastitis / ovine

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Maedi-visna virus infection of ovine mammary epithelial cells

et al. 2006

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“…Lors de cultures in vitro, les cellules de la granulosa (Lamara et al, 2001) comme les cellules épithéliales d'oviductes (Lamara et al, 2002a) ont été démontrées permissives à la réplication du virus. In vivo, l'utilisation combinée de techniques d'immunofluorescence et d'hybridation in situ a permis de démon-trer que les cellules épithéliales utérines était infectées par le CAEV (Ali Al Ahmad et al, 2012b).…”

Section: Risques Liés Au Transfert Embryonnaireunclassified

Evaluation du risque de transmission du virus de l’arthrite encéphalite caprine (CAEV) par les biotechnologies de la reproduction

Fieni¹

2014

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Evaluation of the risk of CAEV (caprine arthritis encephalitis virus) transmission via assisted reproduction techniques Caprine Arthritis-Encephalitis Virus (CAEV) has been identified in the tissues of male and female genital tract, in the semen, in uterine and vaginal secretions, and in embryo flushing medias. So a health risk is associated with artificial insemination (AI), In vitro fecundation (IVF) and embryo transfer (ET). However, it has been demonstrated that sperm fraction, oocytes free of granulosa cells, embryo with intact zona pellicida and after ten washing, take off from naturally infected goats are free of CAEV. So with the respect of definite process, IA, IVF and ET are associated with low level CAEV transmission risk and TE can be use as preventive health measure.

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“…Les animaux malades sont réformés, les animaux infectés et leurs produits (sperme, ovocytes…) (LAMARA et al, 2001;LAMARA et al, 2002) sont susceptibles de limitation à l'exportation lorsqu'ils proviennent d'animaux séropositifs. Ces différentes observations ont conduit à la mise en place en France de deux plans de contrôle ou d'éradication de CAEV d'abord, puis de VMV.…”

Section: • Pourquoi S'intéresser Aux Rétrovirus Animauxunclassified

Rétrovirus et pathologie comparée

Mornex¹,

Chebloune²,

Leroux³

et al. 2003

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Les rétrovirus sont des virus à ARN qui infectent et sont susceptibles de rendre malade l'homme et l'animal. Les infections rétrovirales des petits ruminants et des équidés ont des caractéristiques communes : lorsqu'il existe un diagnostic sérologique, des animaux infectés peuvent être séronégatifs ; les animaux infectés ne sont pas toujours malades ; l'infection et la maladie peuvent être reproduits expérimentalement. Au total, les rétrovirus animaux, en particulier des petits ruminants et des équidés, sont une cause de maladies spontanées, transmissibles chez ces animaux et peuvent être utilisés comme modèle de plusieurs maladies humaines. Mots

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Efficient replication of caprine arthritis-encephalitis virus in goat granulosa cells

Cited by 36 publications

References 30 publications

Maedi-visna virus infection of ovine mammary epithelial cells

Maedi-visna virus infection of ovine mammary epithelial cells

Evaluation du risque de transmission du virus de l’arthrite encéphalite caprine (CAEV) par les biotechnologies de la reproduction

Rétrovirus et pathologie comparée

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