Efficient resynthesis of oilseed rape (Brassica napus L.) from crosses of winter types B. rapa × B. oleracea via simple ovule culture and early hybrid verification

Hilgert-Delgado, Alois Antonín; Klíma, Miroslav; Viehmannová, Iva; Urban, Milan O.; Cusimamani, Eloy Fernández; Vyvadilová, M.

doi:10.1007/s11240-014-0593-2

Cited by 7 publications

(2 citation statements)

References 44 publications

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“…The appearance of mixoploids can be considered as a consequence of endoreduplication (so-called endopolyploidy), a commonly occurring phenomenon in germinating embryos, their cotyledons, and first true leaves in the genus Brassica (see Kudo & Kimura 2001), and/or spontaneous di-or polyploidization during embryogenesis. Therefore, possible overlapping peaks (see Figures 4F, G, I) may complicate the identification of spontaneous or induced doubled haploids (Hilgert et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Klíma,

Shmeit,

Kopecký

et al. 2024

Czech J. Genet. Plant Breed.

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Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids -especially n+2n -were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination.

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Section: Resultsmentioning

confidence: 99%

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Klíma,

Shmeit,

Kopecký

et al. 2024

Czech J. Genet. Plant Breed.

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“…In an inter-specific cross the resultant embryo may be aborted because of parental mutual incompatibility or abnormal meiosis (Brown and Brown, 1996). This can be overcome by immature embryo culture, a process that can rescue inherently weak, immature or hybrid embryos to prevent degeneration (Ćosić et al, 2013;Hilgert-Delgado et al, 2015;Maheswaran and Williams, 1986). The successful application of this technique depends on the stage of the embryo being rescued and cultured in vitro (Finkelstein and Crouch, 1986).…”

Section: Introductionmentioning

confidence: 99%

In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties

Zisan¹,

Robin²,

Hoque³

et al. 2015

J. biosci. agric. res.

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Brassica rapa (AA, 2n = 20) is a prime oilseed species in Bangladesh that can be improved via biotechnological approaches. Rescue of developing embryos after an interspecific hybridization, which otherwise usually tend to abort, is very important for improvement of this species, that require an efficient embryo culture protocol. The immature torpedo shaped embryos (451-700 µm) and mature walking-stick type (>700 µm) embryos of five B. rapa varieties namely, Safal, Agrani, BINA Sarisha-6, BARI Sarisha-6, BARI Sarisha-15 were cultured in basal MS media supplemented with 1 mgL-1 BAP, 0.5 mgL-1 NAA & 1 mgL-1 2,4-D. The immature embryo culture followed indirect somatic embryogenesis process but the mature embryos followed direct organogenesis. The immature embryos induced callus within 11-15 days whereas the mature embryos regenerated plantlets through direct organogenesis within 8-11 days. The varieties Agrani, BARI Sarisha-6 and BINA Sarisha-6 induced the highest percentages of callus from immature embryos and the varieties Safal and BARI Sarisha-15 regenerated the highest percentage of plantlet from mature embryos. Safal and Agrani induced callus and regenerated plantlets comparatively earlier than other varieties. An increase in the concentration of BAP from 10 to 20 ppm in MS media + 5 ppm IBA decreased the size of the plantlets of all varieties except the Agrani. The data could be used in improving the existing embryo rescue protocols. The direct organogenesis process of mature embryos can potentially shorten the breeding cycles.

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Economic/Academic Importance of Brassica rapa

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2015

Compendium of Plant Genomes

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Efficient resynthesis of oilseed rape (Brassica napus L.) from crosses of winter types B. rapa × B. oleracea via simple ovule culture and early hybrid verification

Cited by 7 publications

References 44 publications

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

In vitro callus induction and plantlet regeneration is influenced by the maturity status of embryos of Brassica rapa varieties

Economic/Academic Importance of Brassica rapa

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