Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol

Bader, Araceli Natalia; Rizza, Lara Sánchez; Consolo, Verónica Fabiana; Curatti, Leonardo

doi:10.1016/j.algal.2020.101926

Cited by 26 publications

(7 citation statements)

References 51 publications

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“…Cell wa ll structure, composition and cell size need to be considered, in order to select appropriate hydrolysis methods and improve product recovery [6]. It wa s previously reported that for algal hydrolysis, the best efficiencies of intracellular bioproduct release were achieved using dilute acid and enzymatic methods [7][8][9][10]. While dilute acid methods are fa ster and cheaper, they have the disadvantage of producing fermentation inhibitors [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production

et al. 2021

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“…Thermostable enzymes are thus a potential candidate for industrial application in starch pre-treatment. A study on commercial enzymes for saccharification of algal biomass also showed promising results [ 9 ]. Enzymatic activity depends on various factors such as pH, temperature, salinity and presence of metal ions.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a noble thermostable algal starch liquefying alpha-amylase from Aeribacillus pallidus BTPS-2 isolated from geothermal spring of Nepal

Timilsina

Pandey

Shrestha

et al. 2020

Biotechnology Reports

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A thermophilic strain, Aeribacillus pallidus BTPS-2 was isolated from Bhurung geothermal spring of Nepal. The 16 s rRNA sequence showed 99.8 % similarity with the type strain Aeribacillus pallidus DSM 3670. The morphological, physiological and biochemical properties were similar to the type strain. Alpha-amylase from A. pallidus BTPS-2 was purified to 19-fold purification by DEAE-Cellulose ion exchange chromatography. The K m value of amylase on starch was 0.51 ± 0.05 mg/mL. The optimum pH and temperature were 7.0 and 70 °C. SDS-PAGE analysis showed a single band at 100 kDa. The half-life of the enzyme at 80 °C was 2.81 h. The enzyme showed an inhibitory effect in the presence of Fe 2+ , Pb 2+ , Sn 2+ and Hg 2+ at 10 mM concentrations. TLC analysis showed that the enzyme is a liquifying alpha-amylase. The enzyme reduced the viscosity of algal biomass suspension up to 74.2 ± 0.17 % which was more efficient than Bacillus amyloliquefaciens alpha-amylase (80.5 ± 0.2 %).

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“…pH is one of the factors that affects the nutrient uptake of an organism, cell aggregation, and cell membrane destabilization (Debnath et al, 2021). For this reason, optimum pH should be maintained in algal growth media to achieve maximum biomass and carbohydrate production [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Municipal Landfill Leachate Treatment and Sustainable Ethanol Production: A Biogreen Technology Approach

et al. 2022

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Sustainable material sources are an important agenda to protect the environment and to meet human needs. In this study, Scenedesmus sp. was used to treat municipal landfill leachate via batch and continuous cultivation modes to protect the environment and explore sufficient biomass production for bioethanol production using Saccharomyces cerevisiae. Physicochemical characteristics of leachate were determined for the phases before, during, and after the process. Batch and continuous cultivation were used to treat raw leachate to determine optimum conditions for treatment. Then, the biomass of Scenedesmus sp. with and without sonication was used as a substrate for ethanol production. Sonication was carried out for biomass cell disruption for 20 min at a frequency of 40 kHz. Through batch cultivation mode, it was found that pH 7 was the optimum condition for leachate treatment. Continuous cultivation mode had the highest removal values for COD, phosphorus, and carbohydrate, namely 82.81%, 79.70%, and 84.35%, respectively, among other modes. As for ethanol production, biomass without sonication with 9.026 mg·L−1 ethanol, a biomass concentration of 3.300 µg·L−1, and pH 5 were higher than biomass with sonication with 5.562 mg·L−1 ethanol, a biomass concentration of 0.110 µg·L−1, and pH 5. Therefore, it is evident that the leachate has the potential to be treated by Scenedesmus sp. and converted to bioethanol in line with the concept of sustainable materials.

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Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol

Cited by 26 publications

References 51 publications

Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production

Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae species for bioethanol production

Purification and characterization of a noble thermostable algal starch liquefying alpha-amylase from Aeribacillus pallidus BTPS-2 isolated from geothermal spring of Nepal

Municipal Landfill Leachate Treatment and Sustainable Ethanol Production: A Biogreen Technology Approach

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