Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826

Hols, Pascal; Seksik, Philippe; Dutot, Philippe; Reymund, Jacqueline; Chabot, Paul; Delplace, Brigitte; Delcour, Jean; Mercenier, Annick

doi:10.1099/00221287-143-8-2733

Cited by 51 publications

(54 citation statements)

References 49 publications

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“…Cell wall mutants with a defective alanine racemase gene (Alr Ϫ mutants) have been described for both L. plantarum NCIMB8826 (12) and L. lactis MG1363 (11). When we compared the WT and Alr Ϫ strains, we observed that the growth of both the MD007 and PH3960 mutant strains was impaired after D-alanine starvation, as expected (Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Mucosal Delivery of Antigen with Cell Wall Mutants of Lactic Acid Bacteria

et al. 2004

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The potential of recombinant lactic acid bacteria (LAB) to deliver heterologous antigens to the immune system and to induce protective immunity has been best demonstrated by using the C subunit of tetanus toxin (TTFC) as a model antigen. Two types of LAB carriers have mainly been used, Lactobacillus plantarum and Lactococcus lactis, which differ substantially in their abilities to resist passage through the stomach and to persist in the mouse gastrointestinal tract. Here we analyzed the effect of a deficiency in alanine racemase, an enzyme that participates in cell wall synthesis, in each of these bacterial carriers. Recombinant wild-type and mutant strains of L. plantarum NCIMB8826 and L. lactis MG1363 producing TTFC intracellularly were constructed and used in mouse immunization experiments. Remarkably, we observed that the two cell wall mutant strains were far more immunogenic than their wild-type counterparts when the intragastric route was used. However, intestinal TTFC-specific immunoglobulin A was induced only after immunization with the recombinant L. plantarum mutant strain. Moreover, the alanine racemase mutant of either LAB strain allowed induction of a much stronger serum TTFC-specific immune response after immunization via the vagina, which is a quite different ecosystem than the gastrointestinal tract. The design and use of these mutants thus resulted in a major improvement in the mucosal delivery of antigens exhibiting vaccine properties.

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Section: Resultsmentioning

confidence: 99%

Enhanced Mucosal Delivery of Antigen with Cell Wall Mutants of Lactic Acid Bacteria

et al. 2004

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“…They were obtained by electroporation with a plasmid carrying the TTFC-encoding gene under the control of a constitutive (p ldh ) or inducible (p nisA ) promoter, pMEC4 (erythromycin resistance) or pMEC46 (chloramphenicol resistance), respectively (24; Chagnaud et al, unpublished). A control strain harboring the nonexpressing plasmid pTG2247 (chloramphenicol resistance) was used as a negative control (7). Bacteria were grown in MRS broth (Difco, Detroit, Mich.) containing the appropriate antibiotic, erythromycin or chloramphenicol, at a final concentration of 5 or 10 g per ml, respective.…”

Section: Methodsmentioning

confidence: 99%

Mucosal Immune Responses and Protection against Tetanus Toxin after Intranasal Immunization with RecombinantLactobacillus plantarum

et al. 2001

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The use of live microorganisms as an antigen delivery system is an effective means to elicit local immune responses and thus represents a promising strategy for mucosal vaccination. In this respect, lactic acid bacteria represent an original and attractive approach, as they are safe organisms that are used as food starters and probiotics. To determine whether an immune response could be elicited by intranasal delivery of recombinant lactobacilli, a Lactobacillus plantarum strain of human origin (NCIMB8826) was selected as the expression host. Cytoplasmic production of the 47-kDa fragment C of tetanus toxin (TTFC) was achieved at different levels depending on the plasmid construct. All recombinant strains proved to be immunogenic by the intranasal route in mice and able to elicit very high systemic immunoglobulin G (IgG1, IgG2b, and IgG2a) responses which correlated to the antigen dose. No significant differences in enzyme-linked immunosorbent assay IgG titers were observed when mice were immunized with live or mitomycin C-treated recombinant lactobacilli. Nevertheless, protection against the lethal effect of tetanus toxin was obtained only with the strains producing the highest dose of antigen and was greater following immunization with live bacteria. Significant TTFC-specific mucosal IgA responses were measured in bronchoalveolar lavage fluids, and antigen-specific T-cell responses were detected in cervical lymph nodes, both responses being higher in mice receiving a double dose of bacteria (at a 24-h interval) at each administration. These results demonstrate that recombinant lactobacilli can induce specific humoral (protective) and mucosal antibodies and cellular immune response against protective antigens upon nasal administration.

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“…Some strains of Lactobacillus are found as natural commensals of the gastrointestinal tract (GI tract), the oral cavity and the female uro-genital tract of animals and humans. The ability to adhere to specific regions of the GI tract is a property which has stimulated research aimed at the use of lactobacilli as delivery-vehicles for therapeutic compounds such as immunomodulators, antibodies, enzymes and vaccines (Marteau & Rambaud 1993Hols et al 1997Slos et al 1998). Lactobacillus plantarum arrives in the small intestine in an active state (Vesa et al 2000) and is frequently encountered as a natural inhabitant of the human gastro-intestinal tract (Ahrne et al 1998).…”

Section: Lactobacillus Plantarum Wcfs1 (Contributed By Michiel Kleerementioning

confidence: 99%

Discovering lactic acid bacteria by genomics

Klaenhammer

Altermann

Arigoni

et al. 2002

Lactic Acid Bacteria: Genetics, Metabolism and Applications

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This review summarizes a collection of lactic acid bacteria that are now undergoing genomic sequencing and analysis. Summaries are presented on twenty different species, with each overview discussing the organisms fundamental and practical significance, environmental habitat, and its role in fermentation, bioprocessing, or probiotics. For those projects where genome sequence data were available by March 2002, summaries include 30 a listing of key statistics and interesting genomic features. These efforts will revolutionize our molecular view of Gram-positive bacteria, as up to 15 genomes from the low GC content lactic acid bacteria are expected to be available in the public domain by the end of 2003. Our collective view of the lactic acid bacteria will be fundamentally changed as we rediscover the relationships and capabilities of these organisms through genomics.

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Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826

Cited by 51 publications

References 49 publications

Enhanced Mucosal Delivery of Antigen with Cell Wall Mutants of Lactic Acid Bacteria

Enhanced Mucosal Delivery of Antigen with Cell Wall Mutants of Lactic Acid Bacteria

Mucosal Immune Responses and Protection against Tetanus Toxin after Intranasal Immunization with RecombinantLactobacillus plantarum

Discovering lactic acid bacteria by genomics

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