Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes

Abstract

Selection of Antiviral Immunobiotics in PIE Cells different from those previously reported for the CRL1505 strain in in vitro and in vivo studies. The results of this work confirm that new immunobiotic strains with the ability of stimulating both local and distal antiviral immune responses can be efficiently selected by evaluating the expression of biomarkers in PIE cells.

The Modulation of Mucosal Antiviral Immunity by Immunobiotics: Could They Offer Any Benefit in the SARS-CoV-2 Pandemic?

“…In addition, the selection of new antiviral immunobiotic strains that have different biotechnological properties could enhance the development of various types of functional foods that could be used in nutritional immunobiotic interventions around the world to reduce the incidence and severity of intestinal and pulmonary infections caused by viruses such as the SARS-CoV-2. In this regard, our recent in vitro and in vivo studies demonstrated that L. plantarum MPL16 modulated the intestinal and respiratory antiviral immunity in a similar way as the CRL1505 strain (Albarracin et al, 2020). Whereas, L. rhamnosus CRL1505 has been used mainly in dairy functional products, the MPL16 strain has shown a remarkable ability to growth and ferment wakame (Undaria pinnatifida) that is the most popular edible brown algae in Asian countries.…”

“…In vitro studies performed in intestinal epithelial cells showed that L. rhamnosus CRL1505 was able to improve the production of type I interferons (Villena et al, 2014 ). Moreover, transcriptomic studies revealed a remarkable ability of the CRL1505 strain to enhance the expression of IFN-α and IFN-β as well as the antiviral factors Nplr3, Oas1, OasLA, Mx2 , and RNAseL in intestinal epithelial cells when compared to other immunomodulatory lactobacilli (Albarracin et al, 2017 , 2020 ). In vitro experiments also demonstrated that L. rhamnosus CRL1505 is capable of augmenting the expression of MHC-II and CD80 and the production of IFN-γ in intestinal DCs and macrophages (Villena et al, 2014 ).…”

“…Our results indicated that the immunobiotic treatment was capable of reducing damage to intestinal epithelial cells mediated by the TLR3-IL-15-CD8αα + NKG2D + cells axis (Tada et al, 2016 ). Then, we have conclusively demonstrated that a dietary intervention with L. rhamnosus CRL1505 is able to increase resistance to challenge with intestinal virus, reducing viral replication and favorably modulating the inflammatory response triggered by the viral attack (Villena et al, 2012a , 2014 ; Tada et al, 2016 ; Albarracin et al, 2017 , 2020 ).…”

“…The beneficial effects of L. rhamnosus CRL1505 in the respiratory tract were summarized from Villena et al ( 2012a ), Chiba et al ( 2013 ), and Zelaya et al ( 2014 ). The beneficial effects of L. rhamnosus CRL1505 in the intestinal tract were summarized from Villena et al ( 2014 ), Tada et al ( 2016 ), and Albarracin et al ( 2017 , 2020 ).…”

Immunobiotic Lactobacillus jensenii TL2937 Alleviates Dextran Sodium Sulfate-Induced Colitis by Differentially Modulating the Transcriptomic Response of Intestinal Epithelial Cells

“…Tissue samples (100 mg) were collected from the distal portion of the colon and homogenized in 1 ml of PBS containing protease inhibitors (0.1 mM phenylmethylsulfonyl fluoride, 0.1 mM benzethonium chloride, 10 mM ethylenediaminetetraacetate, 20 KI aprotinin A, and 0.05% Tween 20) and centrifuged for 15 min at 3,000 × g. Blood samples were obtained by cardiac puncture under anesthesia as described previously (26). Intestinal fluid samples were obtained according to Albarracin et al (27). Briefly, the small intestine was flushed with 5 ml of PBS and the fluid was centrifuged (10,000 g, 4 • C for 10 min) to separate particulate material.…”