Efficient slow-growth conservation and assessment of clonal fidelity of Ullucus tuberosus Caldas microshoots

Hammond, Stacy Denise Hammond; Viehmannová, Iva; Zámečnı́k, Jiřı́; Panis, Bart; Čepková, Petra Hlásná

doi:10.1007/s11240-019-01653-z

Cited by 11 publications

(7 citation statements)

References 30 publications

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“…Since the stressful conditions imposed by tissue culture procedures, and their implications on the epi-genetic stability of cultured material, are well known [ 72 , 73 ], many studies using molecular markers have been carried out to examine the genetic stability of in vitro conserved cultures [ 74 , 75 , 76 ]. However, the number of studies focused on the DNA methylation-related stability of slow-growth cultures is scarce ( Table 1 ).…”

Section: In Vitro Plant Conservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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Section: In Vitro Plant Conservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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“…The conservation protocols for Dendrobium draconis, Bulbophyllum auricomum Lindl., Ullucus tuberous species of the subfamily Epidendroideae, Arnica montana L. used MS reduced to half its ionic strength as basal medium (Than, 2013;Hammond Hammond et al, 2019;Menezes-Sá et al, 2019;Petrova et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Another modification made to the culture medium is sorbitol and mannitol as carbon sources. The use of mannitol on slow growth protocols has been reported for Globe artichoke, Ullucus tuberosus (Tavazza et al, 2015;Hammond Hammond et al, 2019). On the other hand, the combined effect of mannitol and sucrose allowed the conservation of Amburana cearensis plants with better characteristics than using sucrose alone (Matos Alvim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The slow growth can be achieved by basal culture medium modification, sucrose, and plant growth regulators (PGRs) concentration changes (Ruta et al, 2020). Other substances used to slow growth are mannitol and sorbitol (da Costa Santos et al, 2011;Tavazza et al, 2015;Hammond Hammond et al, 2019;Muñoz et al, 2019;Petrova et al, 2021;). Furthermore, reports of slow-growth techniques include protocols for conserved material multiplication (Hammond-Hammond et al, 2019;Ramírez-Mosqueda et al, 2019;Fonseka, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Germinación asimbiótica, conservación ex situ e in vitro regeneración de plantas de Catasetum integerrimum Hook

Puc¹,

Cool²

2022

polibotanica

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Podopterus mexicanus Humb. & Bonpl. Polygonaceae. "Rompe capa". Árboles o arbustos de 1.5-6.0 m de altura, ramas con espina terminal, braquiblastos, hojas fasciculadas, flores blancas a verdosas en fascículos, y frutos cubiertos por el perianto externo que forma alas delgadas y largamente decurrentes hacia el pedicelo. Crece en bosques tropicales caducifolios y bosques espinosos, sobre suelo rocoso negro derivado de rocas ígneas. En elevaciones de 550-760 m. Florece de abril a mayo y fructifica de junio a septiembre.

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“…Nodal explants used in all the experiments were derived from rooted shoots propagated in vitro using tip and nodal cutting technique. In vitro propagation through nodal segments has been widely used for plant regeneration and germplasm conservation of medicinal value plants (Hammond et al 2019;Joo et al 2019) due to its advantages related to high rate of multiplication and genetically stability of the regenerated plants.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

Dreger

Gryszczyńska

Szalata

et al. 2020

Plant Cell Tiss Organ Cult

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In this study, a micropropagation protocol using nodal explants from in vitro grown plants of Chamerion angustifolium (L.) Holub was developed and analysis of oenothein B and selected phenolic acids in shoot cultures was performed for the first time. For shoot induction and multiplication Murashige and Skoog’s (MS) basal medium supplemented with 2-isopentenyladenine (2iP), zeatin (Z) and 6-benzyloaminopurine (BAP) was used. 2iP was the most responsive in terms of promoting shoots per explant with the maximum (6.57 ± 1.14) recorded at a concentration of 2.0 mg L−1 after 6 weeks of culture. After two subcultures the multiplication rate was increased up to 19 shoots per explant on medium with 2iP (1.0 mg L−1). To prevent tissue browning, ascorbic acid and casein hydrolysate were added to the induction medium, resulting in a reduction of browning by 30%. The rooted plantlets were successfully transferred to soil and acclimatized with 97% frequency. Quantitative and qualitative assessments of oenothein B and phenolic acid contents in in vitro regenerated shoots as well as in ex vitro plants were performed using high-performance liquid chromatography with a diode-array detector (HPLC-DAD) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC MS/MS) methods. Oenothein B (1.62‒4.55 g 100 g−1 DW), ellagic acid, gallic and caffeic acids were identified in in vitro regenerated plants. The results of this study confirm that the oenothein B-producing plantlets can be obtained using the micropropagation method with axillary shoots being a valuable source of oenothein B and phenolic acids.

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Efficient slow-growth conservation and assessment of clonal fidelity of Ullucus tuberosus Caldas microshoots

Cited by 11 publications

References 30 publications

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Germinación asimbiótica, conservación ex situ e in vitro regeneración de plantas de Catasetum integerrimum Hook

Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

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