Efficient sterilization using reactive oxygen species generated by a radical vapor reactor

Takatsuji, Yoshiyuki; Ishikawa, Shoko; Haruyama, Tetsuya

doi:10.1016/j.procbio.2017.01.002

Cited by 14 publications

(10 citation statements)

References 14 publications

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“…ROS are suitable for sterilization because of their strong oxidation activities. The sterilization effects of ROS by RVR have been reported previously (Takatsuji et al, 2017 ) in a study demonstrating that ROS have stronger sterilization effects than ozone toward exponential cells of E. coli (Gram-negative) and Bacillus subtilis (Gram-positive). In this present study, we effectively sterilized persister cells using the RVR.…”

Section: Introductionsupporting

confidence: 62%

“…Therefore, high concentrations of hydroxyl radical showed strong sterilization effects toward persister cells, and high concentrations of singlet oxygen and low concentrations of hydroxyl radical showed strong sterilization effects toward exponential cells. A previous study also indicated that the vapor condition had higher sterilization effects on cells in the exponential state than the condition without vapor (Takatsuji et al, 2017). It has been reported that viable E. coli persister cells can stop the production of hydroxyl radicals (Kim et al, 2011).…”

Section: Discussionmentioning

confidence: 92%

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Reactive Oxygen Species Penetrate Persister Cell Membranes of Escherichia coli for Effective Cell Killing

Kawano

Yamasaki

Sakakura

et al. 2020

Front. Cell. Infect. Microbiol.

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Persister cells are difficult to eliminate because they are tolerant to antibiotic stress. In the present study, using artificially induced Escherichia coli persister cells, we found that reactive oxygen species (ROS) have greater effects on persister cells than on exponential cells. Thus, we examined which types of ROS could effectively eliminate persister cells and determined the mechanisms underlying the effects of these ROS. Ultraviolet (UV) light irradiation can kill persister cells, and bacterial viability is markedly increased under UV shielding. UV induces the production of ROS, which kill bacteria by moving toward the shielded area. Electron spin resonance-based analysis confirmed that hydroxyl radicals are produced by UV irradiation, although singlet oxygen is not produced. These results clearly revealed that ROS sterilizes persister cells more effectively compared to the sterilization of exponential cells (* * p < 0.01). These ROS do not injure the bacterial cell wall but rather invade the cell, followed by cell killing. Additionally, the sterilization effect on persister cells was increased by exposure to oxygen plasma during UV irradiation. However, vapor conditions decreased persister cell sterilization by reducing the levels of hydroxyl radicals. We also verified the effect of ROS against bacteria in biofilms that are more resistant than planktonic cells. Although UV alone could not completely sterilize the biofilm bacteria, UV with ROS achieved complete sterilization. Our results demonstrate that persister cells strongly resist the effects of antibiotics and starvation stress but are less able to withstand exposure to ROS. It was shown that ROS does not affect the cell membrane but penetrates it and acts internally to kill persister cells. In particular, it was clarified that the hydroxy radical is an effective sterilizer to kill persister cells.

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Section: Introductionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 92%

Reactive Oxygen Species Penetrate Persister Cell Membranes of Escherichia coli for Effective Cell Killing

Kawano

Yamasaki

Sakakura

et al. 2020

Front. Cell. Infect. Microbiol.

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“…The ROS generated by the RVR are effective at the interface between oxygen plasma phase and water phase (O 2 /water interface). [21][22][23] In our previous study, we showed that the top of a droplet was flattened by HFBI self-organization at the air/water interface. [38][39][40][41] After 30 s of RVR treatment, the flat-top HFBI droplet converted to a typical curved droplet (Fig.…”

Section: Area Control Of Ros Dissociation Using a Maskmentioning

confidence: 96%

“…The produced ROS are mainly singlet oxygen ( 1 O 2 ) and hydroxyl radicals (•OH), which were quantitatively obtained in our previous studies. [21][22][23] Ozone and hydrogen peroxide are other types of ROS. These ROS have been used for sterilization and surface treatment because they have high energy and reactivity.…”

Section: Introductionmentioning

confidence: 99%

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Green Surface Cleaning in a Radical Vapor Reactor to Remove Organic Fouling on a Substrate

et al. 2018

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Green cleanup processes for adhered organic fouling on solid surfaces can be successfully performed using a radical vapor reactor (RVR). The RVR can produce large concentrations of reactive oxygen species (ROS, e.g. singlet oxygen (1 O 2) and hydroxyl radicals (•OH)) and can expose them to objective materials. The RVR finds excellent utility in the fields of sterilization and surface functionalization. In this study, RVR is employed in a green cleanup of solid surfaces fouled by an organic polymer and a protein. The RVR produced ROS and removed the adhered organic polymers and proteins from the solid surface. The mechanism of how ROS react with fouling molecules was also elucidated by surface analysis. The greatest advantage of this green RVR cleanup process is that it discharges only air and water. The ROS production and exposure by the RVR successfully cleaned the adhered organic polymer and protein at ambient temperature and pressure without any chemicals. This high-quality, low-cost cleaning technology, which does not require much time and produces no hazardous waste, makes a great contribution to the cleaning industry.

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Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

Seneviratne

Munaweera

Peiris

et al. 2022

Adv Materials Technologies

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Visible light (VL) active silver decorated iron titanate/titanium dioxide (Ag‐FeTiO3/TiO2) nanohybrids derived from natural ilmenite sand effectively cleanse the exophytic plant pathogens in explants grown by in vitro techniques. VL active nanohybrid is synthesized by acid‐hydrolysis of natural ilmenite, followed by precipitation of the lixivium and decoration with Ag onto FeTiO3/TiO2 nanoparticles, and finally calcination under a nitrogen environment to obtain Ag‐FeTiO3/TiO2. A morphological study conducted using transmission electron microscopy (TEM) confirms the formation of Ag‐FeTiO3/TiO2. Powder X‐ray diffraction (PXRD) analysis shows that the nanohybrid primarily consists of anatase, iron titanate (FeTiO3), Ag2O, and Ag. X‐ray photoelectron spectroscopy (XPS) study suggests the presence of Ag0/Ag2O/Fe2O3 composites on the FeTiO3/TiO2 particle surface. The optical band gap significantly changes from 3.14 eV (ilmenite) to 2.80 eV after the decoration of FeTiO3/TiO2 with Ag. This nanohybrid is utilized as a surface‐sterilizing agent for in vitro establishment of the Dracaena sanderiana Sander ex Mast plant. Findings illustrate Ag‐FeTiO3/TiO2 nanohybrid‐based photo‐sterilization leads to the survival of 90% of the microbes’ free cultures while the material can be re‐used due to its photocatalytic behavior. Thus, the newly synthesized nanohybrid can replace harmful sterilization agents used in tissue culture techniques.

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Efficient sterilization using reactive oxygen species generated by a radical vapor reactor

Cited by 14 publications

References 14 publications

Reactive Oxygen Species Penetrate Persister Cell Membranes of Escherichia coli for Effective Cell Killing

Reactive Oxygen Species Penetrate Persister Cell Membranes of Escherichia coli for Effective Cell Killing

Green Surface Cleaning in a Radical Vapor Reactor to Remove Organic Fouling on a Substrate

Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique

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