Efficient synthesis of Ala-Tyr by L-amino acid ligase coupled with ATP regeneration system

Chen, Xiaoping; Du, Xinxin; Zhao, Qiang; Hu, Yingkao; Tian, Caihong; Song, Wenlu

doi:10.1007/s12010-023-04365-5

Cited by 2 publications

(1 citation statement)

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“…TTN cofactor of many regeneration systems have lower values than the system used in our work and even the extension to the regeneration starting from AMP shows comparable values to PPKs, which were successfully implemented in enzymatic syntheses either for AMP or ADP phosphorylation. [46][47][48] The threshold value for TTN cofactor of 100 is surpassed only from a few examples, [12,44,49,50] STY also seldomly reaches the threshold value of 2 g L À 1 h À 1 [12,51,52] and only few examples surpass the TOF threshold of 1 s À 1 . [12,22,42,46,49] Noticeably, often compromises have to be made for the metrics, as some exceed the values of e. g. TTN E , but not for TTN cofactor , TOF or productivity and vice versa.…”

Section: Evaluation Of Atp Regenerating Systemsmentioning

confidence: 96%

Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems

Siedentop,

Prenzel,

Waldvogel

et al. 2023

ChemElectroChem

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Adenosine‐5’‐triphosphate (ATP) plays a crucial role in many biocatalytic reactions and its regeneration can influence the performance of a related enzymatic reaction significantly. Here, we established electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase (ACK) for the phosphorylation of mevalonate catalyzed by mevalonate kinase. A yield of 84 % for the product mevalonate phosphate was reached and a total turnover number for ADP of 68. These metrics are promising for the development of an economic feasible bioprocess and surpass many other enzymatic ATP regeneration systems. A comparison was made to polyphosphate kinases (PPKs), ACK, pyruvate kinase, and creatine kinase in terms of the phosphate donor properties and biocatalytic metrics of exemplary reactions. Furthermore, our system was expanded by a PPK that enables the phosphorylation of AMP, which can broaden the spectrum of applications even further.

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