Efficient transduction of neural cells
            <i>in vitro</i>
            and
            <i>in vivo</i>
            by a baculovirus-derived vector

Sarkis, Chamsy; Serguera, Ché; Pêtres, Stéphane; Buchet, Delphine; Ridet, Jean‐Luc; Edelman, L; Mallet, Jacques

doi:10.1073/pnas.260472897

Cited by 151 publications

(144 citation statements)

References 31 publications

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“…It has been reported that baculovirus vectors produced in insect cells are sensitive to substantial inactivation in vitro by human, rat and guinea-pig serum, and that the vectors have extremely poor infectivity in vivo in mice and rats due to complement inactivation. [28][29][30] By testing gp64-pseudotyped lentiviral vectors, whether the complement sensitivity of baculovirus vectors in rodent serum is due to recognition of the specific envelope glycoprotein or the insect-derived lipid envelope could be evaluated. In this study, the gp64-pseudotyped lentiviral vectors were not inactivated by serum from C57BL/6 mice or Nude rats (Figure 5b).…”

Section: Gp64-pseudotyped Lentiviral Vectors In Vivomentioning

confidence: 99%

Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro

et al. 2004

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Section: Gp64-pseudotyped Lentiviral Vectors In Vivomentioning

confidence: 99%

Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro

et al. 2004

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“…1 Among the various gene delivery vectors that are capable of transducing glial cells is the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based vectors, recently emerging as a new type of gene therapy vehicles. [4][5][6][7][8] Baculoviruses infect astrocytes after being injected into the brain. 6,9 It is worth noting that the brain injection does not offer cell-type specificity: in addition to astrocytes, other types of cells in the brain, including neurons, can be transduced by the injected baculoviruses.…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7][8] Baculoviruses infect astrocytes after being injected into the brain. 6,9 It is worth noting that the brain injection does not offer cell-type specificity: in addition to astrocytes, other types of cells in the brain, including neurons, can be transduced by the injected baculoviruses. 6,[9][10][11] To restrict transgene expression to astrocytes, the promoter of the glial fibrillary acidic protein (GFAP) has been widely used.…”

Section: Introductionmentioning

confidence: 99%

“…6,9 It is worth noting that the brain injection does not offer cell-type specificity: in addition to astrocytes, other types of cells in the brain, including neurons, can be transduced by the injected baculoviruses. 6,[9][10][11] To restrict transgene expression to astrocytes, the promoter of the glial fibrillary acidic protein (GFAP) has been widely used. 12 With its cellular authentic sequence, the promoter is less sensitive to promoter silencing as compared to viral promoters, thus being potentially useful for long-term expression.…”

Section: Introductionmentioning

confidence: 99%

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Astrocytic expression of transgene in the rat brain mediated by baculovirus vectors containing an astrocyte-specific promoter

Wang

2006

Gene Ther

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Therapeutic gene expression in glial cells has been tested for the treatment of neurological diseases in animal models. Many of such studies used the promoter of the glial fibrillary acidic protein (GFAP) to restrict gene expression to astrocytes. We have investigated in the current study whether it is possible to improve the transcriptional activity of the cellular promoter, while maintaining its cell-type specificity. We constructed an expression cassette containing a hybrid cytomegalovirus (CMV) enhancer/GFAP promoter and placed it into baculovirus vectors, a type of viral vectors capable of transducing astrocytes. In another vector design, we used inverted terminal repeats (ITRs) from adeno-associated virus (AAV) to flank the expression cassette. The recombinant baculoviruses with the hybrid promoter improved gene expression levels over two orders of magnitude in glial cell lines and by 10-fold in the rat brain when compared to the baculoviruses with the GFAP promoter alone. The expression was further improved by ITR flanking, reaching levels higher than that mediated by the baculovirus vectors with the CMV immediate-early enhancer/ promoter (CMV promoter). Using these recombinant baculoviruses, we observed extended in vivo transgene expression in the rat brain at 90 days postinjection, by which time the gene expression from baculovirus vectors with the GFAP or CMV promoter had already become undetectable. The astrocyte specificity of the GFAP promoter was preserved in the engineered expression cassette with the CMV enhancer and the AAV ITRs, as demonstrated by immunohistological analysis of brain samples and an axonal retrograde transport assay. Taken together, our findings suggest that these baculovirus vectors may serve as useful tools for astrocyte-specific gene expression in the brain.

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“…The GP64 has been utilized to pseudotype lentiviral vectors to transduce a variety of cell lines in vitro [15]. The GP64 mediated transduction was found to be efficient and therefore pseudotyping of baculovirus vector was reported to be redundant [14]. However, several researchers [9,19] have reported the utility of vesicular stomatitis virus glycoprotein (VSV-G, 511 amino acids long) pseudotyping of baculovirus for gene delivery into mammalian systems.…”

Section: Introductionmentioning

confidence: 99%

Baculovirus mediated transduction: analysis of vesicular stomatitis virus glycoprotein pseudotyping

et al. 2014

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The recombinant baculoviruses were constructed to investigate the necessity of VSV-G pseudotyping for mammalian cell transduction. The viruses were designed to express green fluorescent protein (GFP) gene under the control of cytomegalovirus promoter, with or without pseudotyping with VSV-G. VSV-G was placed under the control of polyhedrin promoter that is recognized by insect cells, allowing the formation of pseudotyped baculovirus. The study findings demonstrate that the pseudotyping of baculovirus significantly enhanced transduction efficiency compared to non-pseudotyped baculovirus, resulting in consequent distinction in the expression of GFP in mammalian cells. The results confirmed that pseudotyping is important for baculovirus mediated gene delivery. Further, when full-length VSV-G pseudotyping was compared with truncated VSV-G containing GED domain (G-stem of ectodomain in conjunction with the TM and CT domains of the glycoprotein), latter was relatively less efficient in transducing mammalian cells. This study demonstrated that pseudotyping with full-length VSV-G had better transduction efficiency in mammalian cells. However, at higher multiplicity of infection, both fulllength and truncated VSV-G showed equivalent transduction. This study established the significance of pseudotyping of baculovirus with full-length VSV-G for efficient transduction of mammalian cells, utilizing the highly sensitive GFP marker system. These findings have significant implications in designing of baculovirus vector based antigen delivery for developing new generation vaccines.

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Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector

Cited by 151 publications

References 31 publications

Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro

Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro

Astrocytic expression of transgene in the rat brain mediated by baculovirus vectors containing an astrocyte-specific promoter

Baculovirus mediated transduction: analysis of vesicular stomatitis virus glycoprotein pseudotyping

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