Efficient transgenic plantlet regeneration from hairy roots via somatic embryogenesis and hardening plantlets of Panax vietnamensis by iron nanoparticles-supplied culture

Nhựt, Dương Tấn; Duc, Huynh Huu; Hoang, Nguyen Hong; Ngan, Ha Thi My; Diem, Le Thi; Tùng, Hoàng Thanh; Khai, Hoang Dac; Mai, Nguyen Thi Nhu; Cường, Đỗ Mạnh; Luận, Vũ Quốc; Tuan, Tran Trong; Giap, Do Dang; Khang, Nguyen Nhat; Binh, Nguyen Van; Hà, Chu Hoàng; Trinh, Thi Bich Ngoc; Hương, Trịnh Thị

doi:10.1007/s11240-022-02355-9

Cited by 5 publications

(1 citation statement)

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“…The literature data overwhelmingly indicate the opposite effect of using NPs on SE. For example, in studies on Ocimum basilicum [ 23 ], Teconella undulata [ 45 , 46 ], Linum usitatissimum [ 22 ], Musa paradisiacal [ 47 ], Gloriosa superba [ 48 ], Coffea arabica [ 49 ], and Panax vietnamensis [ 50 ], NPs improved the efficiency of SE. Data on SE inhibition under the influence of NPs are available but scarce.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Gold Nanoparticles on Somatic Embryogenesis Using the Example of Arabidopsis thaliana

Godel-Jędrychowska

Milewska‐Hendel

Sala

et al. 2023

IJMS

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Although the influence of nanoparticles (NPs) on developmental processes is better understood, little is known about their impact on somatic embryogenesis (SE). This process involves changes in the direction of cell differentiation. Thus, studying the effect of NPs on SE is essential to reveal their impact on cell fate. This study aimed to examine the influence of gold nanoparticles (Au NPs) with different surface charges on the SE of 35S:BBM Arabidopsis thaliana, with particular emphasis on the spatiotemporal localization of pectic arabinogalactan proteins (AGPs) and extensin epitopes in cells changing the direction of their differentiation. The results show that under the influence of nanoparticles, the explant cells of 35S:BBM Arabidopsis thaliana seedling origin did not enter the path of SE. Bulges and the formation of organ-like structures were observed in these explants, in contrast to the control, where somatic embryos developed. Additionally, spatiotemporal changes in the chemical composition of the cell walls during the culture were observed. Under the influence of Au NPs, the following effects were observed: (1) explant cells did not enter the SE pathway, (2) the impacts of Au NPs with different surface charges on the explants were variable, and (3) the compositions of the analyzed pectic AGPs and extensin epitopes were diverse in the cells with different developmental programs: SE (control) and non-SE (treated with Au NPs).

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