Efficient whole-cell biocatalyst for Neu5Ac production by manipulating synthetic, degradation and transmembrane pathways

Zhu, Deqiang; Zhan, Xi; Wu, Jianrong; Gao, Minjie; Zhao, Zhongsheng

doi:10.1007/s10529-016-2215-z

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…Zhu and others found that in this era of extremely rich material life and spiritual life, more and more people like music, like to play music, and like to express their feelings through music. In traditional music performance, people usually get all kinds of wonderful music by playing various musical instruments [7]. Khan et al found that due to the economy, for various reasons, such as playing skills and types of instruments, many people cannot get their favorite instruments to play.…”

Section: Literature Reviewmentioning

confidence: 99%

Construction of Virtual Piano Performance System Based on Visual Gesture Recognition

Tian

2022

Mobile Information Systems

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In order for computers to understand people’s gestures through cameras and react accordingly, through in-depth research, gesture recognition technology in natural human-computer interaction is proposed. Combining natural human-computer interaction technology with music performance, using computer vision-based gestures, music is played in a virtual environment. Experiments show that the virtual piano has 14 piano keys. For the realization of piano performance, it is each piano key; once the piano key is greater than the set value, just call m_Wave.Load() to make a sound. According to the CWave class in the object-oriented MFC class library under VC++, create an object m_Wave of class CWave. Then, according to the m_Wave.Load() function of the CWave class, the connection of the sound is realized. It successfully solves the troubles of music lovers, enriches people’s spiritual life, and has certain practicability and scalability.

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Section: Literature Reviewmentioning

confidence: 99%

Construction of Virtual Piano Performance System Based on Visual Gesture Recognition

Tian

2022

Mobile Information Systems

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“…Although researchers have tried to metabolically engineer a corresponding E. coli strain, only 1.5 g/L Neu5Ac was obtained by fermentation [ 37 ]. In contrast, a de novo two-step biosynthetic pathway for Neu5Ac was designed and assembled in a single E. coli strain; this new pathway co-expressed GlcNAc 2-epimerase (EC 5.1.3.8, AGE) from cyanobacteria and Neu5Ac aldolase (EC 4.1.3.3, NanA) [ 16 , 38 , 39 ] or Neu5Ac synthase (EC 4.1.3.19, NeuB) from bacteria [ 23 , 25 , 40 ]. Whole-cell biocatalysts were developed for Neu5Ac production by assembling a heterologous biosynthetic pathway in E. coli.…”

Section: Whole-cell Biocatalyst Design Principlesmentioning

confidence: 99%

Whole-cell biocatalysts by design

2017

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Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals.

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“…In 2016, Neu5Ac was approved as a food additive in the United States of America and in the European Union and the Republic of China in 2017 [ 7 ]. Due to its importance, Neu5Ac production by enzymatic [ 5 , 8 ] or via whole cell production is still under investigation [ 9 – 11 ]. For this study, the enzymatic synthesis was chosen for its simple reaction sequence ( Figure 1 ) and high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Shift of the reaction equilibrium at high pressure in the continuous synthesis of neuraminic acid

Reich¹,

Aßmann²,

Hölting³

et al. 2022

Beilstein J. Org. Chem.

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The importance of a compound that helps fight against influenza is, in times of a pandemic, self-evident. In order to produce these compounds in vast quantities, many researchers consider continuous flow reactors in chemical industry as next stepping stone for large scale production. For these reasons, the synthesis of N-acetylneuraminic acid (Neu5Ac) in a continuous fixed-bed reactor by an immobilized epimerase and aldolase was investigated in detail. The immobilized enzymes showed high stability, with half-life times > 173 days under storage conditions (6 °C in buffer) and reusability over 50 recycling steps, and were characterized regarding the reaction kinetics (initial rate) and scalability (different lab scales) in a batch reactor. The reaction kinetics were studied in a continuous flow reactor. A high-pressure circular reactor (up to 130 MPa) was applied for the investigation of changes in the position of the reaction equilibrium. By this, equilibrium conversion, selectivity, and yield were increased from 57.9% to 63.9%, 81.9% to 84.7%, and 47.5% to 54.1%, respectively. This indicates a reduction in molar volume from N-acetyl-ᴅ-glucosamine (GlcNAc) and pyruvate (Pyr) to Neu5Ac. In particular, the circular reactor showed great potential to study reactions at high pressure while allowing for easy sampling. Additionally, an increase in affinity of pyruvate towards both tested enzymes was observed when high pressure was applied, as evidenced by a decrease of KI for the epimerase and KM for the aldolase from 108 to 42 mM and 91 to 37 mM, respectively.

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Efficient whole-cell biocatalyst for Neu5Ac production by manipulating synthetic, degradation and transmembrane pathways

Abstract: A three-module whole-cell biocatalyst strategy by manipulating synthetic, degradation and transmembrane pathways in E. coli was an economical method for Neu5Ac production.

Cited by 12 publications

References 19 publications

Construction of Virtual Piano Performance System Based on Visual Gesture Recognition

Construction of Virtual Piano Performance System Based on Visual Gesture Recognition

Whole-cell biocatalysts by design

Shift of the reaction equilibrium at high pressure in the continuous synthesis of neuraminic acid

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