Efflux Pump Inhibition Effect of Curcumin and Phenylalanine Arginyl β- Naphthylamide (PAβN) against Multidrug Resistant Pseudomonas Aeruginosa Isolated from Burn Infections in Tanta University Hospitals

Eshra, Kareman Ahmed; Shalaby, Marwa M.

doi:10.12816/0046279

Cited by 8 publications

(11 citation statements)

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“…Ballard et al showed that the use of natural curcumin in combination with antibiotics can decrease the MICs of antibiotics (31). Another study showed that as the concentration of curcumin rises, its effectiveness as an inhibitor increases (13). In our study, 54 to 100% of aminoglycoside-resistant isolates showed a decrease in the MICs of these antibiotics after curcumin addition.…”

Section: Discussionsupporting

confidence: 53%

“…The mechanism of action of this substance is competitive inhibition, as efflux pumps in the bacteria detect and remove it instead of the target antibiotics (fluoroquinolones, mainly ciprofloxacin and levofloxacin) from the cells. As a result, antibiotics remain in the cell and increase in concentration (13). Curcumin is also a natural inhibitor derived from turmeric and has been proven to have anti-cancer, anti-inflammatory, and antimicrobial effects against viruses, bacteria, and pathogenic fungi (14).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Charkhi

Haghshenas

Mirzaei

et al. 2020

J Adv Med Biomed Res

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Background & Objective: Efflux pump inhibitors (EPIs) can block efflux pumps and are helpful in potentiating the activity of aminoglycosides against Pseudomonas aeruginosa. The present study compared the effects of phenylalanine-arginine beta naphthylamide (PAβN) and curcumin on aminoglycoside minimum inhibitory concentration (MIC) on Pseudomonas aeruginosa clinical isolates. Materials & Methods: For this descriptive-analytical study, 100 clinical isolates of Pseudomonas aeruginosa were collected and identified by differential diagnostic tests. The MICs of amikacin, gentamicin, and tobramycin were evaluated before and after adding EPIs using a micro-broth dilution test. Results: The bacteria were isolated from different types of samples, including urine (26 isolates), sputum (37 isolates), ulcers (20 isolates), catheters (eight isolates), blood (five isolates), feces (two isolates), and eyes (two isolates). Overall, 60% of the isolates were obtained from males (mean age = 47.85), and 40% from females (mean age = 44.76). In the MIC test, 11 (25.5%), 15 (34.8%), and 18 (41.8%) isolates were resistant to amikacin, gentamicin, and tobramycin, respectively. Significant reductions in the MICs of amikacin, gentamicin, and tobramycin were observed after adding curcumin in 54-100% of aminoglycoside-resistant isolates, while fewer changes in the MICs of aminoglycosides were seen against these clinical isolates after adding PAβN (36-55%). Conclusion: Curcumin and PAβN can potentiate the effect of aminoglycosides on clinical isolates of Pseudomonas aeruginosa and change their susceptibility pattern due to efflux pump inhibition. However, our outcomes detected that curcumin was more effective than the PAβN against the aminoglycoside-resistant isolates of P. aeruginosa.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Charkhi

Haghshenas

Mirzaei

et al. 2020

J Adv Med Biomed Res

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“…Another mechanism that may be contributing to the bacteria resistance is the efflux pumps, as is suggested by the results observed in the strains PE20 and PE64, which showed a decrease of CMI for MEM in presence of EPI, as has been reported in other studies. 26 These results also suggest that other resistance mechanisms, eg, the role of AmpC, 50 could be involved.…”

Section: Discussionmentioning

confidence: 82%

“… 25 Also, it has been reported that the pump inhibitor phenylalanine arginyl β-naphthylamide (EPI), allows the participation of these pumps in the resistance to meropenem and fluoroquinolones, through a mechanism of competitive inhibition, this is because the pumps recognize EPI as a substrate instead of the target antibiotics. 25 , 26 …”

Section: Introductionmentioning

confidence: 99%

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

López-García¹,

Rocha-Gracia²,

Bello-López³

et al. 2018

IDR

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PurposePseudomonas aeruginosa infections in hospitals constitute an important problem due to the increasing multidrug resistance (MDR) and carbapenems resistance. The knowledge of resistance mechanisms in Pseudomonas strains is an important issue for an adequate antimicrobial treatment. Therefore, the objective was to investigate other antimicrobial resistance mechanisms in MDR P. aeruginosa strains carrying blaIMP, make a partial plasmids characterization, and determine if modifications in oprD gene affect the expression of the OprD protein.MethodologySusceptibility testing was performed by Kirby Baüer and by Minimum Inhibitory Concentration (presence/absence of efflux pump inhibitor); molecular typing by Pulsed-field gel electrophoresis (PFGE), resistance genotyping and integrons by PCR and sequencing; OprD expression by Western blot; plasmid characterization by MOB Typing Technique, molecular size by PFGE-S1; and blaIMP location by Southern blot.ResultsAmong the 59 studied P. aeruginosa isolates, 41 multidrug resistance and carbapenems resistance isolates were detected and classified in 38 different PFGE patterns. Thirteen strains carried blaIMP; 16 blaGES and four carried both genes. This study centered on the 17 strains har-boring blaIMP. New variants of β-lactamases were identified (blaGES-32, blaIMP-56, blaIMP-62) inside of new arrangements of class 1 integrons. The presence of blaIMP gene was detected in two plasmids in the same strain. The participation of the OprD protein and efflux pumps in the resistance to carbapenems and quinolones is shown. No expression of the porin OprD due to stop codon or IS in the gene was found.ConclusionsThis study shows the participation of different resistance mechanisms, which are reflected in the levels of MIC to carbapenems. This is the first report of the presence of three new variants of β-lactamases inside of new arrangements of class 1 integrons, as well as the presence of two plasmids carrying blaIMP in the same P. aeruginosa strain isolated in a Mexican hospital.

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“…Plant sources used since ancient time to fight microbial infections appear as an interesting alternative for the discovery of new antibacterial substances against MDR bacteria [ 10 – 12 ]. For instance, a number of plant-deriving compounds with possible efflux pump inhibitor (EPI) activities such as reserpine [ 13 ], berberine [ 13 , 14 ], and curcumin [ 15 ] have been discovered, but they are still unused clinically. In our previous studies, we have also shown that edible plants could be used alone and/or in combination with commonly used antibiotics to fight infections involving Gram-negative MDR bacteria [ 16 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiotic-Potentiating Activities of Thirteen Cameroonian Edible Plants against Gram-Negative Resistant Phenotypes

Nayim

Mbaveng

Wamba

et al. 2018

The Scientific World Journal

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This work was designed to investigate the antibacterial activities of methanol extracts from thirteen Cameroonian edible plants and their antibiotic-potentiating effects against Gram-negative multidrug-resistant (MDR) phenotypes. The broth microdilution method was used to evaluate the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extracts, as well as their antibiotic-potentiating activities. The phytochemical screening of the extracts was carried out according to the standard methods. The results of phytochemical tests revealed the presence of sterols, polyphenols, and tannins in most of the tested extracts, with the other classes of secondary metabolites being selectively distributed. Tested extracts showed variable antibacterial activities with MIC values ranging from 64 to 1024 μg/mL. However, some extracts were significantly active against certain bacterial strains: seeds extract of Theobroma cacao (64 μg/mL) against Escherichia coli AG100Atet and Klebsiella pneumoniae K24, and the bark extract of Uapaca guineensis against E. coli ATCC 8739. The leaves extract of T. cacao displayed the best MBC values (256 μg/mL) against E. aerogenes EA27. Some tested extracts included extracts from the leaves of T. cacao and P. vulgaris, and the seeds of D. edulis and barks A. indica has selectively improved (2- to 64-fold) the antibacterial activities of some of the tested antibiotics, chloramphenicol (CHL), tetracycline (TET), kanamycin (KAN), streptomycin (STR), and erythromycin (ERY), against more than 70% of tested MDR bacteria. The findings of this work showed that tested plant extracts and particularly those from T. cacao and Phaseolus vulgaris can be used alone or in combination with conventional antibiotics in the treatment of infections involving multiresistant bacteria.

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Efflux Pump Inhibition Effect of Curcumin and Phenylalanine Arginyl β- Naphthylamide (PAβN) against Multidrug Resistant Pseudomonas Aeruginosa Isolated from Burn Infections in Tanta University Hospitals

Cited by 8 publications

References 0 publications

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Characterization of antimicrobial resistance mechanisms in carbapenem-resistant <em>Pseudomonas aeruginosa</em> carrying IMP variants recovered from a Mexican Hospital

Antibacterial and Antibiotic-Potentiating Activities of Thirteen Cameroonian Edible Plants against Gram-Negative Resistant Phenotypes

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