“…The total soluble phenols (TSP) content was quantified with the Folin-Ciocalteu (F-C) method (Singleton and Rossi, 1965) adapted to a microplates reader (Salgado-Escobar et al, 2020). Based on this, 25 µL of extract, 125 µL of distilled water, 20 µL of F-C reagent diluted at 1:10 ratio, and 30 µL of 20% sodium carbonate were mixed; agitation and rest were applied for 30 min in the dark, and absorbance was measured at 760 nm with an UV/Vis spectrophotometer.…”