EgAP2-1, an AINTEGUMENTA-like (AIL) gene expressed in meristematic and proliferating tissues of embryos in oil palm

Morcillo, Fabienne; Gallard, Anthony; Pillot, Marion; Jouannic, Stéfan; Aberlenc-Bertossi, Frédérique; Collin, Myriam; Verdeil, Jean‐Luc; Tregear, James

doi:10.1007/s00425-007-0574-3

Cited by 56 publications

(45 citation statements)

References 42 publications

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“…Transient expression of both Bbm and Wus2 in the same cells often produced growth stimulation greater than that by Bbm or Wus2 alone, with a mixed phenotype in which apparently embryogenic and organogenic structures were intermingled ( Figure 1F). In dicots, ectopic expression of BBM has been reported to produce somatic embryos in various species and tissue types, including Arabidopsis cotyledons (Boutilier et al, 2002), Arabidopsis leaves (Morcillo et al, 2007;Bandupriya et al, 2014), Brassica napus leaves (Boutilier et al, 2002), and callus from Populus tomentosum (Deng et al, 2009). However, none of these reports indicates any stimulation of transformation frequencies.…”

Section: Discussionmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.

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Section: Discussionmentioning

confidence: 99%

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

et al. 2016

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“…While the exact function of BBM is still unclear, the data suggest a role for BBM in either the induction and/or the maintenance of embryo development. Data from another BBM-like gene, EgAP2-1, isolated from Elaeis guineensis (oil palm), suggest that it also may play a role in oil palm zygotic and somatic embryo development (Morcillo et al, 2007). The complete gene sequences and gene prediction of the four ASGR-BBMlike genes suggests that they are potentially functional.…”

Section: Discussion Sequence Analysis Of Asgr and Asgr-recombinant Bamentioning

confidence: 99%

Sequence Analysis of Bacterial Artificial Chromosome Clones from the Apospory-Specific Genomic Region ofPennisetumandCenchrus

et al. 2008

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Apomixis, asexual reproduction through seed, is widespread among angiosperm families. Gametophytic apomixis in Pennisetum squamulatum and Cenchrus ciliaris is controlled by the apospory-specific genomic region (ASGR), which is highly conserved and macrosyntenic between these species. Thirty-two ASGR bacterial artificial chromosomes (BACs) isolated from both species and one ASGR-recombining BAC from P. squamulatum, which together cover approximately 2.7 Mb of DNA, were used to investigate the genomic structure of this region. Phrap assembly of 4,521 high-quality reads generated 1,341 contiguous sequences (contigs; 730 from the ASGR and 30 from the ASGR-recombining BAC in P. squamulatum, plus 580 from the C. ciliaris ASGR). Contigs containing putative protein-coding regions unrelated to transposable elements were identified based on protein similarity after Basic Local Alignment Search Tool X analysis. These putative coding regions were further analyzed in silico with reference to the rice (Oryza sativa) and sorghum (Sorghum bicolor) genomes using the resources at Gramene (www.gramene.org) and Phytozome (www.phytozome.net) and by hybridization against sorghum BAC filters. The ASGR sequences reveal that the ASGR (1) contains both gene-rich and gene-poor segments, (2) contains several genes that may play a role in apomictic development, (3) has many classes of transposable elements, and (4) does not exhibit large-scale synteny with either rice or sorghum genomes but does contain multiple regions of microsynteny with these species.

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“…The overexpression of native or heterologous BBM genes also induces regeneration in other species (Morcillo et al, 2007;Deng et al, 2009;El Ouakfaoui et al, 2010;Heidmann et al, 2011;Lutz et al, 2011;Bandupriya et al, 2014;Yang et al, 2014;Florez et al, 2015;Lowe et al, 2016) and, therefore, is used as a biotechnology tool to improve plant transformation in model and crop species (Deng et al, 2009;Heidmann et al, 2011;Lutz et al, 2011;Florez et al, 2015;Lowe et al, 2016).…”

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confidence: 99%

The BABY BOOM Transcription Factor Activates the LEC1-ABI3-FUS3-LEC2 Network to Induce Somatic Embryogenesis

et al. 2017

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Somatic embryogenesis is an example of induced cellular totipotency, where embryos develop from vegetative cells rather than from gamete fusion. Somatic embryogenesis can be induced in vitro by exposing explants to growth regulators and/or stress treatments. The BABY BOOM (BBM) and LEAFY COTYLEDON1 (LEC1) and LEC2 transcription factors are key regulators of plant cell totipotency, as ectopic overexpression of either transcription factor induces somatic embryo formation from Arabidopsis (Arabidopsis thaliana) seedlings without exogenous growth regulators or stress treatments. Although LEC and BBM proteins regulate the same developmental process, it is not known whether they function in the same molecular pathway. We show that BBM transcriptionally regulates LEC1 and LEC2, as well as the two other LAFL genes, FUSCA3 (FUS3) and ABSCISIC ACID INSENSITIVE3 (ABI3). LEC2 and ABI3 quantitatively regulate BBM-mediated somatic embryogenesis, while FUS3 and LEC1 are essential for this process. BBM-mediated somatic embryogenesis is dose and context dependent, and the contextdependent phenotypes are associated with differential LAFL expression. We also uncover functional redundancy for somatic embryogenesis among other Arabidopsis BBM-like proteins and show that one of these proteins, PLETHORA2, also regulates LAFL gene expression. Our data place BBM upstream of other major regulators of plant embryo identity and totipotency.

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EgAP2-1, an AINTEGUMENTA-like (AIL) gene expressed in meristematic and proliferating tissues of embryos in oil palm

Cited by 56 publications

References 42 publications

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

Sequence Analysis of Bacterial Artificial Chromosome Clones from the Apospory-Specific Genomic Region ofPennisetumandCenchrus

The BABY BOOM Transcription Factor Activates the LEC1-ABI3-FUS3-LEC2 Network to Induce Somatic Embryogenesis

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