EGCG ameliorates the hypoxia-induced apoptosis and osteogenic differentiation reduction of mesenchymal stem cells via upregulating miR-210

Qiu, Yiyan; Yang, Chen; Zeng, Tenghui; Guo, Weizhuang; Zhou, Wenyu; Yang, Xinjian

doi:10.1007/s11033-015-3936-0

Cited by 26 publications

(15 citation statements)

References 54 publications

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“…At the same concentration, EGCG can also decrease osteoclastogenesis via the regulation of osteoprotegrin (OPG) and the receptor activator of Nuclear factor-kB (RANK) ligand (RANKL) in pre-osteoclast feeder cells, ST2 [32]. Qiu's study also showed that 10-40 µmol/L of EGCG significantly inhibited hypoxia-induced apoptosis in BMSCs and increased the level of runt-related transcription factor 2 (Runx2), BMP-2, type I procollagen, and alkaline phosphatase activity [33]. We found No published data regarding the effect dose of EGCG on fracture healing.…”

Section: Discussionmentioning

confidence: 99%

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

et al. 2020

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Green tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGCG can also enhance the osteogenic differentiation of murine bone marrow mesenchymal stem cells and inhibit the osteoclastogenesis in RAW264.7 cells by modulation of the receptor activator of nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegrin (OPG) (RANK/RANKL/OPG) pathway. Our previous study also found that EGCG can promote bone defect healing in the distal femur partially via bone morphogenetic protein-2 (BMP-2). Considering the osteoinduction property of BMP-2, we hypothesized that EGCG could accelerate the bone healing process with an increased expression of BMP-2. In this manuscript, we studied whether the local use of EGCG can facilitate tibial fracture healing. Fifty-six 4-month-old rats were randomly assigned to two groups after being weight-matched: a control group with vehicle treatment (Ctrl) and a study group with 10 µmol/L, 40 µL, EGCG treatment (EGCG). Two days after the operation, the rats were treated daily with EGCG or vehicle by percutaneous local injection for 2 weeks. The application of EGCG enhanced callus formation by increasing the bone volume and subsequently improved the mechanical properties of the tibial bone, including the maximal load, break load, stiffness, and Young’s modulus. The results of the histology and BMP-2 immunohistochemistry staining showed that EGCG treatment accelerated the bone matrix formation and produced a stronger expression of BMP-2. Taken together, this study for the first time demonstrated that local treatment of EGCG can accelerate the fracture healing process at least partly via BMP-2.

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Section: Discussionmentioning

confidence: 99%

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

et al. 2020

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“…Therefore, miR‐210 might accelerate the malignant progression of OS through promoting the dedifferentiation of OS cells. There is evidence that miR‐210 promotes the osteoblastic differentiation of bone marrow mesenchymal stem cells by targeting activin A receptor type 1B (AcvR1b) and ephrin‐A3 (EFNA3) (Mizuno et al, ; Qiu et al, ). Based on our previous analysis of gene expression (GSE38135), there was no change in the expression of AcvR1b or EFNA3 during the dedifferentiation of OS (data not shown).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐210 is increased and it is required for dedifferentiation of osteosarcoma cell line

Zhang

Mai

Chen

2017

Cell Biology International

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Osteosarcoma (OS) is the most common malignant bone tumor and is prevalent in adolescents. In clinical studies, miR-210 has been reported to be tightly correlated to the poor prognosis of OS. Nevertheless, its roles in OS have not been fully elucidated. In view of the central role played by OS stem cells (OSCs) in the malignant progression of OS, this study investigated the influence of miR-210 on the formation of OSCs. Our previous findings suggested that the microenvironment of bone, abundant TGF-β1 and hypoxia, could induce OS cells to dedifferentiate into OSCs. In this study, we found that miR-210 participated in the dedifferentiation of OS cells into OSCs, and inhibiting it significantly suppressed the formation of OSCs. Further results suggested that miR-210 promoted the expression of TGF-β1 and its downstream effectors Snail1 and Slug which were highly elevated in the process of OS dedifferentiation. Additionally, the target gene of miR-210 was also investigated. It was found that NFIC was significantly reduced by miR-210 treatment and also during OS dedifferentiation. Therefore, this study suggested that miR-210 promoted OS cells dedifferentiation and uncovered its role in the malignant progress of OS.

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“…The fact that the amount of polyphenols found in the yerba mate infusion are greater than its amount found in blended tea support this hypothesis (Heck & de Mejia, ). Epigallocatechin, which a high amount is also is found in green tea, has been showing a favorable effect on bone metabolism in experimental studies (Qiu et al, ). Moreover, subjects that have an intake of three or more cups per day of black tea were found to have fewer fractures than subjects with a lesser intake (Myers et al, ).…”

Section: Discussionmentioning

confidence: 99%

The yerba mate intake has a neutral effect on bone: A case–control study in postmenopausal women

Veiga

Bringhenti

Bolignon

et al. 2017

Phytotherapy Research

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Nutritional factors have been associated with osteoporosis and fractures. The intake of coffee may increase the risk of fracture whereas the intake of black and green tea is associated with its reduction. Recently, consumption of yerba mate was associated with increased bone mineral density in postmenopausal women. Nonetheless, its influence on fracture is not known. The aim of this study was to evaluate the effect of yerba mate tea intake on fractures, bone markers, calcium homeostasis, and oxidative stress in postmenopausal women. A case-control study was carried out in South Brazil, 46 women with fractures and 49 controls completed the study. There was no significant difference between the frequency of fractures in women who drank mate tea and women who did not (48.3% vs. 48.5%, p = .99). Moreover, there was no significant difference concerning the serum levels of total calcium, phosphorus, PTH, vitamin D, P1NP, and CTX in the subjects with the history of yerba mate use when compared to controls. Higher serum levels of NOx were found in women who drank the yerba mate infusion. In conclusion, the yerba mate intake is not associated with fracture, and it appears to have a neutral effect on the bone metabolism.

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EGCG ameliorates the hypoxia-induced apoptosis and osteogenic differentiation reduction of mesenchymal stem cells via upregulating miR-210

Cited by 26 publications

References 54 publications

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

MicroRNA‐210 is increased and it is required for dedifferentiation of osteosarcoma cell line

The yerba mate intake has a neutral effect on bone: A case–control study in postmenopausal women

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