Ege Üniversitesi Hastanesi'ne başvuran hastalarda enzim işaretli floresan test ile elde edilen Epstein-Barr virüsü serolojik test sonuçlarının değerlendirilmesi

Soylu, Mustafa Kemal; Zeytinoğlu, Ayşın; Altuğlu, İmre

doi:10.19161/etd.344069

Cited by 2 publications

(3 citation statements)

References 3 publications

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“…Fidan et al [15] detected that 91.4% of the patients were IgG seropositive. Soylu et al [16] reported that the rate of IgG seropositive patients was 81%. The data of our study suggests that acute infection rate was 6.1% and IgG seropositivity rate was 86.6%, seronegativity rate was 7.3%.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

Görgün

Havuz

Mehel³

2020

Preprint

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Background: The aim of this study was to diagnose Epstein-Barr virüs (EBV) infection using indirect immunofluorescence assay (IIFA) method and to evaluate these patients clinically. More than 90% of children are infected with EBV until the age of six. Methods: The tests were studied by using EBV IIFA method. A total of 247 patients, 186 adults, and 61 children, were included in the study. Results: 5 (2.7%) of the adults were EBV-Capsid antigen (EBV-CA) IgM positive, 175 (94.1%) were IgG positive and 6 (3.2%) were seronegative. 10 (16.4%) of the child patients were IgM positive, which is considered as an acute infectious mononucleosis (IM) infection marker, whereas the child patients had a significantly higher IgM rate than adults (p <0.001). 39 (63.9%) of the child patients were IgG positive and 12 (19.7%) of them were seronegative. The rate of IgG positivity in children was significantly lower than in adults (p <0.001).There was no significant difference between the genders in terms of IgM and IgG positivity rates in both adults and children(p> 0.05 for each).Conclusion: These results suggest that the presence of an acute EBV infection should be considered when the patient has viral diseases with similar clinical picture especially for children.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

Görgün

Havuz

Mehel³

2020

Preprint

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“…(6,15). İzole VCA IgG pozitifliği farklı çalışmalarda %1.7-7.9 arasında değişen oranlarda bildirilmektedir (9,10,(15)(16)(17). Ayrıca izole VCA IgG pozitifliğinin heterofil antikor pozitifliğiyle ilişkisi söylenerek, çocuklarda özellikle 10 yaş altında daha çok akut infeksiyonu desteklediği bildirilmiştir.…”

Section: Yöntemlerunclassified

“…İzole EBNA-1 IgG pozitifliği, VCA IgG üretiminde yetersizlik meydana gelmesi veya antikorların zamanla kaybolması gibi nedenlerle gelişebilmektedir ve farklı çalışmalarda %0.8-5.3 arasında değişen oranlarda bildirilmektedir (6,9,10,17,21…”

Section: Yöntemlerunclassified

Investigation of Epstein-Barr Virus Antibodies by Chemiluminescent Microparticle Immunoassay Method in Patients of Different Age Groups: Evaluation of Atypical Serological Profiles

Duran¹,

Ati²

2021

Klimik Derg

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Objective: Epstein-Barr virus (EBV) can cause different clinical pictures from infectious mononucleosis (IM) to malignancies such as B-cell lymphomas, Burkitt’s lymphoma, nasopharyngeal carcinoma, and Hodgkin lymphoma. VCA-IgM, VCA-IgG, EBNA-1 IgG antibodies are the most commonly used antibodies in revealing the serological profile. This study aimed to examine the serological profiles of patients with suspected EBV infection and to interpret the atypical profiles encountered. Methods: The results of VCA-IgM, VCA-IgG, and EBNA-1 IgG antibodies studied in the Microbiology Laboratory between 2017-2019 were evaluated retrospectively. EBV serological tests (VCA-IgM, VCA-IgG, and EBNA-1 IgG) were performed according to the manufacturer’s recommendations using the chemiluminescent microparticle immunoassay (CMIA) method (Architect, Abbott, Wiesbaden, Germany). Results: Of the 2486 patients evaluated, 1341 (53.9%) were male, 1145 (46.1%) were female, and the average age was determined as 16.93 ± 19.5. EBV past infection was detected in 56.65% of the cases, the acute infection was detected in 17.25%, and 21.09% did not encounter EBV. Atypical serological profile was detected in 5.01%. As an atypical profile, the most common positivity of three antibodies together (3.90%), then isolated VCA-IgG positivity (0.91%), and isolated EBNA-1 IgG positivity (0.20%) were determined. It was determined that 24.24% of the cases with an atypical profile were immunosuppressive patient. Conclusions: The rate of encountering EBV in our study is 78.91%. The atypical EBV serological profile rate was found to be 5.01%. Approximately one-fourth of the cases with an atypical profile was found to be in the patient group with immune disorders. It is thought that antibody tests are not sufficient to determine the stage of infection, especially in these patient groups, and further tests should be performed. It has been demonstrated that serological monitoring is required for the interpretation of atypical profiles. Keywords: Epstein-Barr virüs, serological tests, chemiluminescent microparticle immunoassay (CMIA), atypical serological profile

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Ege Üniversitesi Hastanesi'ne başvuran hastalarda enzim işaretli floresan test ile elde edilen Epstein-Barr virüsü serolojik test sonuçlarının değerlendirilmesi

Cited by 2 publications

References 3 publications

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

Evaluation of Epstein-Barr Virus Indirect Immunofluorescence Assay Results

Investigation of Epstein-Barr Virus Antibodies by Chemiluminescent Microparticle Immunoassay Method in Patients of Different Age Groups: Evaluation of Atypical Serological Profiles

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