EGFR in enterocytes &amp; endothelium and HIF1α in enterocytes are dispensable for massive small bowel resection induced angiogenesis

Onufer, Emily J.; Aladegbami, Bola; Imai, Toru; Seiler, Kristen M.; Bajinting, Adam; Courtney, Cathleen; Sutton, Stephanie; Bustos, Aiza; Yao, Junjie; Yeh, Cheng-Hung; Sescleifer, Anne M.; Wang, Lihong V.; Guo, Jun; Warner, Brad W.

doi:10.1371/journal.pone.0236964

Cited by 3 publications

(3 citation statements)

References 56 publications

(70 reference statements)

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“…Anti-promyelocytic leukemia zinc finger (PLZF) (rabbit anti-PLZF antibody, 1/400, Sigma-Aldrich, HPA001499, St. Louis, MO, USA) was employed to identify undifferentiated spermatogonia [ 62 ]. Vascular structures and mature vascular structures were labeled, applying antibodies directed, respectively, towards α-SMA (anti-alpha smooth muscle actin, ab5694, Abcam, Cambridge, UK) [ 63 ] and CD31 (Cluster of differentiation-31) (PECAM-1/D8V9E- XP Rabbit, Cell Signaling, Danvers, MA, USA) [ 64 ]. Slides were incubated for 60 min at RT with a secondary anti-rabbit antibody (Envision+ system-labeled polymer-horseradish peroxidase (HRP); DAKO, K4003, Agilent, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles

Vento

Poels

Vermeulen

et al. 2021

IJMS

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Avascular transplantation of frozen–thawed testicular tissue fragments represents a potential future technique for fertility restoration in boys with cancer. A significant loss of spermatogonia was observed in xeno-transplants of human tissue most likely due to the hypoxic period before revascularization. To reduce the effect of hypoxia–reoxygenation injuries, several options have already been explored, like encapsulation in alginate hydrogel and supplementation with nanoparticles delivering a necrosis inhibitor (NECINH) or VEGF. While these approaches improved short-term (5 days) vascular surfaces in grafts, neovessels were not maintained up to 21 days; i.e., the time needed for achieving vessel stabilization. To better support tissue grafts, nanoparticles loaded with VEGF, PDGF and NECINH were developed. Testicular tissue fragments from 4–5-week-old mice were encapsulated in calcium-alginate hydrogels, either non-supplemented (control) or supplemented with drug-loaded nanoparticles (VEGF-nanoparticles; VEGF-nanoparticles + PDGF-nanoparticles; NECINH-nanoparticles; VEGF-nanoparticles + NECINH-nanoparticles; and VEGF-nanoparticles + PDGF-nanoparticles + NECINH-nanoparticles) before auto-transplantation. Grafts were recovered after 5 or 21 days for analyses of tissue integrity (hematoxylin–eosin staining), spermatogonial survival (immuno-histo-chemistry for promyelocytic leukemia zinc finger) and vascularization (immuno-histo-chemistry for α-smooth muscle actin and CD-31). Our results showed that a combination of VEGF and PDGF nanoparticles increased vascular maturity and induced a faster maturation of vascular structures in grafts.

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Section: Methodsmentioning

confidence: 99%

Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles

Vento

Poels

Vermeulen

et al. 2021

IJMS

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“…Besides enhanced enterocyte proliferation, it has been revealed that adaptation is associated with angiogenesis. In fact, prominent angiogenesis leads to improved blood circulation in the gastrointestinal tract [ 29 , 30 , 31 ].…”

Section: Intestinal Adaptationmentioning

confidence: 99%

An Overview of Short-Bowel Syndrome in Pediatric Patients: Focus on Clinical Management and Prevention of Complications

2023

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Short-bowel syndrome (SBS) in pediatric age is defined as a malabsorptive state, resulting from congenital malformations, significant small intestine surgical resection or disease-associated loss of absorption. SBS is the leading cause of intestinal failure in children and the underlying cause in 50% of patients on home parental nutrition. It is a life-altering and life-threatening disease due to the inability of the residual intestinal function to maintain nutritional homeostasis of protein, fluid, electrolyte or micronutrient without parenteral or enteral supplementation. The use of parenteral nutrition (PN) has improved medical care in SBS, decreasing mortality and improving the overall prognosis. However, the long-term use of PN is associated with the incidence of many complications, including liver disease and catheter-associated malfunction and bloodstream infections (CRBSIs). This manuscript is a narrative review of the current available evidence on the management of SBS in the pediatric population, focusing on prognostic factors and outcome. The literature review showed that in recent years, the standardization of management has demonstrated to improve the quality of life in these complex patients. Moreover, the development of knowledge in clinical practice has led to a reduction in mortality and morbidity. Diagnostic and therapeutic decisions should be made by a multidisciplinary team that includes neonatologists, pediatric surgeons, gastroenterologists, pediatricians, nutritionists and nurses. A significant improvement in prognosis can occur through the careful monitoring of nutritional status, avoiding dependence on PN and favoring an early introduction of enteral nutrition, and through the prevention, diagnosis and aggressive treatment of CRSBIs and SIBO. Multicenter initiatives, such as research consortium or data registries, are mandatory in order to personalize the management of these patients, improve their quality of life and reduce the cost of care.

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“…Understanding interactions between light and medium is a longstanding issue in biomedical imaging [1,2], as it has fundamental importance to imaging techniques such as photoacoustic imaging [3][4][5][6]. Optical resolution photoacoustic microscopy (ORPAM) is a photoacoustic imaging modality, which has demonstrated diffraction-limited lateral resolution on the surface [7], subwavelength-resolution label-free imaging [8], and subdiffraction label-free imaging [9], despite typically limited penetration depth of 1-2 mm due to the tight optical focus of ballistic photons [10].…”

Section: Introductionmentioning

confidence: 99%

Temporal Evolution of Refractive Index Induced by Short Laser Pulses Accounting for Both Photoacoustic and Photothermal Effects

Xia

et al. 2022

Applied Sciences

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Materials such as silicon, copper, gold, and aluminum exhibit strong absorption and scattering characterization under short-pulsed laser irradiation. Due to the photoelastic effect and thermoelastic relaxation, the focal area may induce a local modulation in the refractive index, which can be detected with the intensity reflection coefficient perturbation. Normally, the thermal effect causes a weak refractive index change and is negligible, compared with the pressure-induced effect in most photoacoustic analytical systems. In this study, we present a theoretical model with the whole process of absorbed energy conversion analysis for the refractive index perturbation induced by both thermal effect and photoacoustic pressure. In this model, data analysis was carried out on the transformation of the energy absorbed by the sample into heat and stress. To prove the feasibility of this model, numerical simulation was performed for the photothermal and photoacoustic effects under different incident intensities using the finite element method. Experiment results on silicon and carbon fiber verified that the refractive index change induced by the photothermal effect can be detected and be incorporated with pressure-induced refractive index change. The simulation results showed very good agreement with the results of the experiments. The main aim of this study was to further understand the absorption and conversion process of short-pulsed light energy and the resulting photothermal and photoacoustic effects.

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EGFR in enterocytes & endothelium and HIF1α in enterocytes are dispensable for massive small bowel resection induced angiogenesis

Cited by 3 publications

References 56 publications

Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles

Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles

An Overview of Short-Bowel Syndrome in Pediatric Patients: Focus on Clinical Management and Prevention of Complications

Temporal Evolution of Refractive Index Induced by Short Laser Pulses Accounting for Both Photoacoustic and Photothermal Effects

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