EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Parry, Jean M.; Velarde, Nathalie V.; Lefkovith, Ariel; Zegarek, Matthew H.; Hang, Julie S.; Ohm, Jonathan T.; Klancer, Richard; Maruyama, Rika; Druzhinina, Marina; Grant, Barth D.; Piano, Fabio; Singson, Andrew

doi:10.1016/j.cub.2009.09.015

Cited by 73 publications

(130 citation statements)

References 15 publications

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“…EGG-4/5 also requires EGG-3 for proper localization. Without EGG-4/5, MBK-2 is not localized to the cortex and embryos lacking EGG-4/5, which have defects in meiosis, polar body formation, F-actin dynamics, and eggshell deposition (73).…”

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

“…EGG-3 binds MBK-2 and, after meiosis, co-localizes with it in the cortex of oocytes and in cytoplasmic puncta (72,74,75). EGG-3 plays a central role in spatial localization and temporal regulation of molecules that are directly involved in executing the oocyte-to-embryo transition (72)(73)(74).…”

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

“…The oocyte cortex plays a critical role in regulating fertilization and establishing embryonic polarity (14,72,73). Our current understanding is that EGG-3, EGG-4/5, and MBK-2 bind to each other and form a complex at the oocyte cortex (Fig.…”

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

“…EGG-3, EGG-4, and EGG-5 are considered pseudo-phosphatases because they contain an inactive protein tyrosine phosphatase (PTP) domain that lacks a key residue in the catalytic active site (72,73,76). 62% of all C. elegans and 40% of human PTP-domain proteins are thought to be pseudo-phosphatases (77).…”

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

“…EGG-3, EGG-4, and EGG-5 are pseudo-phosphatases in the oocyte that regulate MBK-2 activity, a kinase known to regu-http://bmbreports.org late the oocyte-to-embryo transition (72)(73)(74)(75). EGG-3, EGG-4, and EGG-5 are considered pseudo-phosphatases because they contain an inactive protein tyrosine phosphatase (PTP) domain that lacks a key residue in the catalytic active site (72,73,76).…”

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

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Fertilization and the oocyte-to-embryo transition in C. elegans

Marcello¹,

Singson²

2010

BMB Reports

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Fertilization is a complex process comprised of numerous steps. During fertilization, two highly specialized and differentiated cells (sperm and egg) fuse and subsequently trigger the development of an embryo from a quiescent, arrested oocyte. Molecular interactions between the sperm and egg are necessary for regulating the developmental potential of an oocyte, and precise coordination and regulation of gene expression and protein function are critical for proper embryonic development. The nematode Caenorhabditis elegans has emerged as a valuable model system for identifying genes involved in fertilization and the oocyte-to-embryo transition as well as for understanding the molecular mechanisms that govern these processes. In this review, we will address current knowledge of the molecular underpinnings of gamete interactions during fertilization and the oocyte-to-embryo transition in C. elegans. We will also compare our knowledge of these processes in C. elegans to what is known about similar processes in mammalian, specifically mouse, model systems. [BMB reports 2010; 43(6): 389-399]

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Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

Section: The Oocyte-to-embryo Transitionmentioning

confidence: 99%

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Fertilization and the oocyte-to-embryo transition in C. elegans

Marcello¹,

Singson²

2010

BMB Reports

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The eggshell in the C. elegans oocyte‐to‐embryo transition

Johnston

Dennis

2011

Genesis

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Summary: In egg-laying animals, embryonic development takes place within the highly specialized environment provided by the eggshell and its underlying extracellular matrix. Far from being simply a passive physical support, the eggshell is a key player in many early developmental events. Herein, we review current understanding of eggshell structure, biosynthesis, and function in zygotic development of the nematode, C. elegans. Beginning at sperm contact or entry, eggshell layers are produced sequentially. The earlier outer layers are required for secretion or organization of inner layers, and layers differ in composition and function. Developmental events that depend on the eggshell include polyspermy barrier generation, high fidelity meiotic chromosome segregation, osmotic barrier synthesis, polar body extrusion, anterior-posterior polarization, and organization of membrane and cortical proteins. The C. elegans eggshell is proving to be an excellent, tractable system to study the molecular cues of the extracellular matrix that instruct cell polarity and early development. genesis 50:333-349, 2012. V V C 2011 Wiley Periodicals, Inc.

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The molecular underpinnings of fertility: Genetic approaches in Caenorhabditis elegans

Mei

Singson

2020

Advanced Genetics

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The study of mutations that impact fertility has a catch-22. Fertility mutants are often lost since they cannot simply be propagated and maintained. This has hindered progress in understanding the genetics of fertility. In mice, several molecules are found to be required for the interactions between the sperm and egg, with JUNO and IZUMO1 being the only known receptor pair on the egg and sperm surface, respectively. In Caenorhabditis elegans, a total of 12 proteins on the sperm or oocyte have been identified to mediate gamete interactions. Majority of these genes were identified through mutants isolated from genetic screens. In this review, we summarize the several key screening strategies that led to the identification of fertility mutants in C. elegans and provide a perspective about future research using genetic approaches. Recently, advancements in new technologies such as high-throughput sequencing and Crispr-based genome editing tools have accelerated the molecular, cell biological, and mechanistic analysis of fertility genes. We review how these valuable tools advance our understanding of the molecular underpinnings of fertilization. We draw parallels of the molecular mechanisms of fertilization between worms and mammals and argue that our work in C. elegans complements fertility research in humans and other species.

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EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Cited by 73 publications

References 15 publications

Fertilization and the oocyte-to-embryo transition in C. elegans

Fertilization and the oocyte-to-embryo transition in C. elegans

The eggshell in the C. elegans oocyte‐to‐embryo transition

The molecular underpinnings of fertility: Genetic approaches in Caenorhabditis elegans

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