Egg consumption improves vascular and gut microbiota function without increasing inflammatory, metabolic, and oxidative stress markers

Liu, Xiang; Shao, Yijia; Sun, Jinhua; Tu, Jiazichao; Wang, Zhichao; Tao, Jun; Chen, Jimei

doi:10.1002/fsn3.2671

Cited by 17 publications

(17 citation statements)

References 54 publications

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“…Importantly, eggs and their components have been reported to improve gut microbiota. A recent study reported that egg consumption improved gut microbiota [140]. Fermented egg-milk beverage also increased beneficial bacteria from Lachnospiraceae and Lactobacillus [141].…”

Section: Eggsmentioning

confidence: 95%

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

Chen¹,

Vitetta²

2022

Front. Biosci. (Schol Ed)

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The main entry point of SARS-CoV-2 is the respiratory tract and as such immune defence in this site determines if the virus will spillover to the systemic circulation and circulate and infect other major organs. The first line of mucosal immune defence is composed of mucins, an epithelial barrier, and immune cells in the nasal cavity. The lung immune defence is carried out by numerous alveoli. The lung microbiota is a key factor in determining the efficacy of lung mucosal immunity protection. The intestinal microbiota has been demonstrated to affect the severity of COVID-19. Gut dysbiosis is involved in hyperinflammation and multiple organ failure through communications with multiple organs. The gut lung axis could be the earliest axis affected in COVID-19. Through the gut-lung axis, gut dysbiosis can affect the pathogenesis of the lung in COVID-19. In this review, we summarise the effects that gut dysbiosis can progress on the lung, and the lung microbiota. The possible mechanisms and approaches for modulation are discussed.

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Section: Eggsmentioning

confidence: 95%

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

Chen¹,

Vitetta²

2022

Front. Biosci. (Schol Ed)

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“…TMAO contained in the Exos was detected on a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system consisting of an Agilent 1260 high-performance liquid chromatography (HPLC) and 6420 triple quadrupole mass spectrometer with an electrospray ionization source (ESI) (Agilent), which was performed according to the standard protocols as described in our previous study (Liu et al, 2021a). Briefly, 100 μl of the specimen was mixed with the internal standard working solution (d9-TMAO), and 300 μl of acetonitrile was added to the sample and then vortexed, and the mixture was centrifuged at 15,294×g for 5 min.…”

Section: Detection Of Trimethylamine-n-oxidementioning

confidence: 99%

TMAO-Activated Hepatocyte-Derived Exosomes Impair Angiogenesis via Repressing CXCR4

Xiang

Shao

et al. 2022

Front. Cell Dev. Biol.

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Objective: Trimethylamine-N-oxide (TMAO) was found to play crucial roles in vascular endothelial function. However, the exact molecular mechanisms are not yet entirely clear. Recently, we found that exosomes (Exos) isolated from TMAO-treated hepatocytes (TMAO-Exos) contained a distinctive profile of miRNAs compared to those from the TMAO-free group (Control-Exos). Furthermore, TMAO-Exos could notably promote inflammation, damage vascular endothelial cells (VECs), and impair endothelium-dependent vasodilation. This study aimed to further evaluate the effects of TMAO-Exos on VECs and explore the underlying mechanisms.Methods: Exos were isolated from the hepatocyte culture supernatant with or without TMAO, using differential centrifugation. Then, VECs were treated with these Exos for 48 h and subjected to RNA-sequencing for detecting the changes of alternative polyadenylation (APA) and mRNA. After validation by qPCR and western blotting, the recombinant viruses were used to mediate the overexpression of C-X-C motif chemokine receptor 4 (CXCR4). The in vitro VEC function was evaluated by cell migration and tube formation, and in vivo angiogenesis was investigated in hindlimb ischemia models.Results: Exos released from hepatocytes were differentially regulated by TMAO; both could be taken up by VECs; and furthermore, TMAO-Exos significantly reduced cell migration and tube formation in vitro and impaired perfusion recovery and angiogenesis after hindlimb ischemia, by down-regulating the CXCR4 expression. However, TMAO-Exos failed to regulate the splicing events, at least in this experimental setting, which suggested that TMAO-Exos may affect CXCR4 expression via an APA-independent manner.Conclusions: Our findings revealed a novel indirect mechanism by which TMAO impaired endothelial function through stimulating hepatocytes to produce Exos that possessed distinctive activity. The crosstalk between the liver and vascular endothelial mediated by these Exos may offer a new target for restraining the harmful effects induced by TMAO.

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“…Brachial-ankle PWV and endothelial function improved, with no effect on BP, inflammation, oxidative stress, or TMAO. Microbiota was not modified, but a reduction of tryptophan degradation was observed [ 91 ].…”

Section: Systematic Reviewmentioning

confidence: 99%

The Gut Microbiota and Vascular Aging: A State-of-the-Art and Systematic Review of the Literature

Agnoletti

Piani

Cicero

et al. 2022

JCM

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The gut microbiota is a critical regulator of human physiology, deleterious changes to its composition and function (dysbiosis) have been linked to the development and progression of cardiovascular diseases. Vascular ageing (VA) is a process of progressive stiffening of the arterial tree associated with arterial wall remodeling, which can precede hypertension and organ damage, and is associated with cardiovascular risk. Arterial stiffness has become the preferred marker of VA. In our systematic review, we found an association between gut microbiota composition and arterial stiffness, with two patterns, in most animal and human studies: a direct correlation between arterial stiffness and abundances of bacteria associated with altered gut permeability and inflammation; an inverse relationship between arterial stiffness, microbiota diversity, and abundances of bacteria associated with most fit microbiota composition. Interventional studies were able to show a stable link between microbiota modification and arterial stiffness only in animals. None of the human interventional trials was able to demonstrate this relationship, and very few adjusted the analyses for determinants of arterial stiffness. We observed a lack of large randomized interventional trials in humans that test the role of gut microbiota modifications on arterial stiffness, and take into account BP and hemodynamic alterations.

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Egg consumption improves vascular and gut microbiota function without increasing inflammatory, metabolic, and oxidative stress markers

Cited by 17 publications

References 54 publications

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

TMAO-Activated Hepatocyte-Derived Exosomes Impair Angiogenesis via Repressing CXCR4

The Gut Microbiota and Vascular Aging: A State-of-the-Art and Systematic Review of the Literature

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