Egg stickiness in artificial reproduction of sturgeon: an overview

Siddique, Mohammad Abdul Momin; Pšenička, Martin; Cosson, Jacky; Dzyuba, Borys; Rodina, Marek; Golpour, Amin; Linhart, Otomar

doi:10.1111/raq.12070

Cited by 30 publications

(18 citation statements)

References 74 publications

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“…Concurrently, the gas exchange decrease between the eggs (Siddique et al . ). The pikeperch eggs and majority of fish oocytes are surrounded with the external proteinaceous layer called the egg envelope or chorion (Cherr & Clark ; Robles, Cabrita, de Paz & Herraez ), which are the main source of egg stickiness.…”

Section: Discussionmentioning

confidence: 97%

“…In most cultured species, for example, tench, common carp, pike and pikeperch, hatching rate is an important criterion to evaluate the efficacy of artificial reproduction. In the hatchery, the success of artificial reproduction partially depends on the incubation and elimination of egg stickiness (Demska- Zakes et al 2005;Siddique et al 2014;Zarski et al 2015). The stickiness of eggs is not problematic for fertilization, but during incubation, eggs become attached to one another and increase fungal diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Elimination of egg stickiness is one of the most important factors for incubation of eggs in hatchery jars (e.g. Zug jars), which increase hatching rate and larval production (Linhart, Gela, Flaj shans, Duda, Rodina & Nov ak 2000;Gela, Linhart, Flajshans & Rodina 2003;Siddique, Psenicka, Cosson, Dzyuba, Rodina, Golpour & Linhart 2014).…”

Section: Introductionmentioning

confidence: 99%

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Alcalase treatment for elimination of stickiness in pikeperch (Sander luciopercaL.) eggs under controlled conditions

2015

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Elimination of egg stickiness is an important factor in artificial reproduction of pikeperch (Sander lucioperca L.). This study was conducted to evaluate the effectiveness of Alcalase enzyme to remove the adhesive layer of pikeperch eggs. The eggs were treated with Alcalase at 0.5, 1.0, 1.5, 2.0 and 5.0 mL L À1 or a milk/talc solution 2 min post insemination. Duration of exposure was 2 min in Alcalase and 60 min in milk/talc. The highest, albeit not significant, hatching rate (85.4%) was found with 1.5 mL L À1 of Alcalase, but hatching rates were similar in 0.5, 1.0 and 2.0 mL L À1 . Hatching rates were significantly lower groups treated with 5.0 mL L À1 Alcalase enzyme (56.4%) compared to groups treated with milk powder and talc (61.3%). Nominally complete removal of adhesiveness was observed in 1.5 and 2 mL L À1 . All Alcalase treatments led to significantly lower incubation duration compared with the traditional milk/talc treatment. The application of Alcalase successfully eliminated pikeperch egg stickiness in less time than with traditional milk/clay/talc methods.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Alcalase treatment for elimination of stickiness in pikeperch (Sander luciopercaL.) eggs under controlled conditions

2015

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“…The presence of only one such structure is common in teleost fish, and is a characteristic used to differentiate the oocytes from other species with multiple micropyles like Sturgeon (Psenicka et al, 2011;Siddique et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

Murgas¹,

Paulino²,

Palhares³

et al. 2017

fisheriessciences

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Abstract:This study was designed to identify and measure the gametes of neotropical fish using ultrastructural analysis. Number, shape and size of micropyle and ultrastructure of egg membrane and of the micropyle canal; the number and length of the longest and shortest ridge in the micropyle region beyond the structures of the sperm cell have been used as taxonomic tool in ichthyology . Gametes of six breeding pairs of Piaractus mesopotamicus, Brycon orbignyanus, Salminus brasiliensis and Prochilodus lineatus were collected, fixed and subjected to scanning electron microscopy for the observation and measurement of the structures. The results showed many similarities between the gametes of the studied species. An array of grooves and folds was observed in the micropyle in all species but not in the Prochilodus lineatus oocytes. The micropyle was funnel-shaped in all species. The oocytes showed a great variation in the diameters of major and minor micropyle ostium, evidencing different reproductive strategies. The sperm cells showed simple structures with ovoide heads, a cylindrical midpiece with a single flagellum and no acrosome. This study may contribute to a better understanding of fish reproductive biology, conservation genetics and breeding studies. Eight hours after the second CECP dose, fish were removed from the aquarium and had their urogenital papilla cleaned and dried with a towel. The collection of gametes began after delicate manual massage of the coelomic wall. This procedure was performed in accordance with the Ethical Principles of Animal Experimentation, Protocol 040/2009 of Lavras Federal University-UFLA. Keywords Ultrastructural analysis of gametesPost-fixation methodology was accomplished in accordance to the methodology employed in the Laboratory of Eletronic Microscopy and Ultrastructural Analysis of Lavras Federal University-UFLA. Samples containing 1mL of semen or 30 oocytes of each species were fixed separately in microtubules containing a solution of 2.5% glutaraldehyde and 2.0% paraformaldehyde in 0.05 M sodium cacodylate buffer, pH 7.2, calcium chloride 0.001 M. The samples were maintained for 24 hours at 4°C. After this, samples were immersed in 1% osmium tetroxide for 4 hours at room temperature and subsequent dehydration through in ascending series of acetone (25%, 50%, 75%, 90% and 100%).Before evaluation in the electron scanning microscope, the oocytes and spermatozoa were dehydrated with a CPD030 critical point instrument. The samples were coated with gold in a SCD 050 vacuum evaporator according to protocols designed in Ultrastructural Analysis and Electronic Microscopy Laboratory of Federal University of Lavras, Brazil. Morphometric analysis of the gametesIn the morphometry of the oocytes, was valued the major diameter (Md), minor diameter (md), total volume and the sperm head width ratio and the major diameter of major micropyle ostium (MSH/MO). In the morphometry of the micropyle (Figure 1) in the oocytes surface, was measured the diameters of the major ostium (MO) and minor...

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“…A 2‐min heat shock (at 34°C) was then given at 18 min post‐activation (Fopp‐Bayat et al., ). Sturgeon eggs are strongly adhesive; it is highly recommended to remove the egg adhesiveness after fertilization (Siddique et al., ). Therefore eliminating the egg stickiness was performed with NaCl–tannic acid solutions (Feledi, Kucska, & Ronyai, ).…”

Section: Methodsmentioning

confidence: 99%

Effects of feeding rate on the growth performance of gynogenetic albino sterlet,Acipenser ruthenus(Linnaeus, 1758) larvae

et al. 2017

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Summary The feeding rate effects were studied on the growth performance of gynogenetic diploid larvae of sterlet Acipenser ruthenus during the first 4 weeks of exogenous feeding. The experimental rearing was conducted from 7 to 38 days post‐hatch (dph) in a circulation system. This was set up in four groups with three replicates (440 individuals/replicate), viz: AC‐control larvae fed Artemia sp., CFC‐control larvae fed compound feed, AG‐gynogenetic larvae fed Artemia sp., and CFG‐gynogenetic larvae fed compound feed. The larvae were reared in glass tanks (44 L volume, 10 individuals/L) with the temperature maintained at 18 ± 0.5°C, photoperiod of 12L:12D and water flow regime of 1‐L/min and fed 50%, 25%, 25%, and 9% of their total biomass/day during feeding. Highest TL and WBW of gynogenetic diploid larvae (AG) were observed with 50.6 ± 1.2 mm and 607.3 ± 36.1 mg (n = 30) at 38 dph. Highest TL and WBW of control larvae (AC) were recorded with 49.5 ± 3.8 mm and 600.8 ± 88.0 mg (n = 30), respectively, with 73.1% ± 11.4% survival; the lowest survival rate was at 46.4% ± 7.1% (n = 30) for the CFG group. The results indicate that the gynogenetic and normal larvae of sterlet fed with live food (Artemia nauplii) from 7 dph can achieve higher growth and survivability compared to the larvae fed with formulated test feed. Results of this study suggest that the effective rearing of sterlet larvae from 7 to 38 dph strongly depends upon the types of feed rather than the genome manipulation performed.

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Egg stickiness in artificial reproduction of sturgeon: an overview

Cited by 30 publications

References 74 publications

Alcalase treatment for elimination of stickiness in pikeperch (Sander luciopercaL.) eggs under controlled conditions

Alcalase treatment for elimination of stickiness in pikeperch (Sander luciopercaL.) eggs under controlled conditions

Ultrastructural and Morphometric Analysis of Gametes in Neotropical Teleost Fishes

Effects of feeding rate on the growth performance of gynogenetic albino sterlet,Acipenser ruthenus(Linnaeus, 1758) larvae

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