Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Jantrakajorn, Sasibha; Wongtavatchai, Janenuj

doi:10.17221/8523-cjas

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…Bronopol is widely used in cosmetics along with in air and water sterilization (Xing, Chang, & Wei, 2010). While bronopol has been applied to aquatic disease control in some reports, its application to aquatic organisms is rare (Jantrakajorn & Wongtavatchai, 2015;Picón-Camacho, Taylor, Bron, Fu, & Shinn, 2012), and no detection method has been established to detect bronopol in fish. In this study, we established a bronopol detection method and evaluated the pharmacokinetics of bronopol in grass carp for the first time.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and tissue distribution of bronopol in grass carp, Ctenopharyngodon Idella , at 15 and 20°C

Zhan

Fang

et al. 2019

Aquac Res

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This study explored the pharmacokinetic characteristics of bronopol in grass carp (Ctenopharyngodon idella) under different temperatures. The concentrations of bronopol in the plasma and tissues (kidney, liver and muscle) of grass carp after soaking in bronopol (6.75 µg/ml) at 15 and 20°C were determined using liquid chromatography–mass spectrometry. The concentrations of bronopol in both plasma and tissue first increased and then decreased with soaking time at both tested temperatures. The peak bronopol concentrations at 15°C (plasma: 8.934 µg/ml; kidney: 9.23 µg/g; liver: 9.47 µg/g; and muscle: 7.98 µg/g) were slightly lower than those at 20°C (plasma: 9.654 µg/ml; kidney: 10.83 µg/g; liver: 13.40 µg/g; and muscle: 8.68 µg/g). In an analysis of pharmacokinetic parameters, the bronopol concentration–time profiles for plasma were best described by a two‐compartment, open pharmacokinetic model with first‐order absorption. The t1/2β for plasma at 15 and 20°C was 82.933 and 92.041 hr, respectively, indicating that the elimination of bronopol was faster at 15°C than at 20°C. In addition, AUC0‐t and AUC0‐∞ values of bronopol were 477.892 and 495.809 µg/L hr at 15°C and 495.809 and 589.859 µg/L hr at 20°C, indicating that the content of bronopol in the organism was higher at higher temperature.

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Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and tissue distribution of bronopol in grass carp, Ctenopharyngodon Idella , at 15 and 20°C

Zhan

Fang

et al. 2019

Aquac Res

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“…In the manufacturing industry, bronopol is commonly used in agricultural fungicides, cosmetic preservatives, water-treatment fungicides and petroleum industry fungicides [ 11 , 12 , 13 ]. It has been discovered that the number of bacteria on tilapia eggs could be significantly reduced when the fertilized tilapia eggs were soaked in bronopol for a certain period of time [ 14 ]. In a study conducted on rainbow trout, the incidence of fungal infection in the eggs of rainbow trout decreased after being treated with 100 mg/L of bronopol for 30 min [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Characterizing the Mechanisms of Metalaxyl, Bronopol and Copper Sulfate against Saprolegnia parasitica Using Modern Transcriptomics

Wang

Fei

et al. 2022

Genes

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Saprolegniasis, which is caused by Saprolegnia parasitica, leads to considerable economic losses. Recently, we showed that metalaxyl, bronopol and copper sulfate are good antimicrobial agents for aquaculture. In the current study, the efficacies of metalaxyl, bronopol and copper sulfate are evaluated by in vitro antimicrobial experiments, and the mechanism of action of these three antimicrobials on S. parasitica is explored using transcriptome technology. Finally, the potential target genes of antimicrobials on S. parasitica are identified by protein–protein interaction network analysis. Copper sulfate had the best inhibitory effect on S. parasitica, followed by bronopol. A total of 1771, 723 and 2118 DEGs upregulated and 1416, 319 and 2161 DEGs downregulated S. parasitica after three drug treatments (metalaxyl, bronopol and copper sulfate), separately. Additionally, KEGG pathway analysis also determined that there were 17, 19 and 13 significantly enriched metabolic pathways. PPI network analysis screened out three important proteins, and their corresponding genes were SPRG_08456, SPRG_03679 and SPRG_10775. Our results indicate that three antimicrobials inhibit S. parasitica growth by affecting multiple biological functions, including protein synthesis, oxidative stress, lipid metabolism and energy metabolism. Additionally, the screened key genes can be used as potential target genes of chemical antimicrobial drugs for S. parasitica.

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FELASA-AALAS Recommendations for Biosecurity in an Aquatic Facility, Including Prevention of Zoonosis, Introduction of New Fish Colonies, and Quarantine

Mocho¹,

Collymore²,

Farmer³

et al. 2022

comp med

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FELASA and AALAS established a joint working group to advise on good practices for the exchange of fish for research. In a first manuscript, the working group made recommendations for health monitoring and reporting of monitoring results. The focus of this second related manuscript is biosecurity in fish facilities. First, we define the risk of contamination of personnel by zoonotic pathogens from fish or from system water, including human mycobacteriosis. Preventive measures are recommended, such as wearing task-specific personal protective equipment. Then we discuss biosecurity, highlighting the establishment of biosecurity barriers to preserve the health status of a facility. A functional biosecurity program relies on integration of the entire animal facility organization, including the flow of staff and animals, water treatments, and equipment sanitation. Finally, we propose 4 steps for introducing new fish colonies: consideration of international trade and national restrictions; assessing risk according to fish source and developmental stage; establishing quarantine barriers; and the triage, screening, and treatment of newly imported fish. We then provide 3 realistic sample scenarios to illustrate practical biosecurity risk assessments and mitigation measures based on considerations of health status and quarantine conditions.

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Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticus

Cited by 4 publications

References 28 publications

Pharmacokinetics and tissue distribution of bronopol in grass carp, Ctenopharyngodon Idella , at 15 and 20°C

Pharmacokinetics and tissue distribution of bronopol in grass carp, Ctenopharyngodon Idella , at 15 and 20°C

Characterizing the Mechanisms of Metalaxyl, Bronopol and Copper Sulfate against Saprolegnia parasitica Using Modern Transcriptomics

FELASA-AALAS Recommendations for Biosecurity in an Aquatic Facility, Including Prevention of Zoonosis, Introduction of New Fish Colonies, and Quarantine

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