Egg-to-Embryo Transition Is Driven by Differential Responses to Ca2+ Oscillation Number

Ducibella, Tom; Huneau, D.; Angelichio, Elizabeth Lloyd; Xu, Zhe; Schultz, Richard M.; Kopf, Gregory S.; Fissore, Rafael A.; Madoux, S.; Ozil, Jean-Pierre

doi:10.1006/dbio.2002.0788

Cited by 362 publications

(217 citation statements)

References 50 publications

Supporting

Mentioning

206

Contrasting

Unclassified

Order By: Relevance

“…Subsequent IP 3 binding to its own receptor on the endoplasmic reticulum (ER) [19] opens Ca 2+ channels, which release Ca 2+ from the ER into the cytoplasm of the oocyte [19]. The resulting Ca 2+ oscillations within the oocyte [18,19] disseminate from the cortical region throughout the ooplasm [20,21] at 10-to 20-min intervals [22], triggering oocyte activation [23] to initiate oocyte fertilization and preimplantation embryogenesis [8,14,17].…”

Section: Discussionmentioning

confidence: 99%

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report

Chithiwala

Lee

Hill

et al. 2015

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose The purpose of this study is to describe impaired oocyte fertilization from phospholipase C-zeta (PLC-ζ) deficiency in normal-appearing sperm that was successfully treated using calcium (Ca 2+ ) ionophore with intracytoplasmic sperm injection (ICSI) of oocytes matured in vitro. Methods An infertile couple undergoing in vitro fertilization (IVF) experienced failed oocyte fertilization following ICSI with normal-appearing sperm. A semen sample collected from the patient was used to assess the expression of sperm PLC-ζ protein by Western blot analysis and immunofluorescence and PLC-ζ bioactivity by an in vitro model of Ca 2+ release. A second IVF cycle was performed using Ca 2+ ionophore with ICSI to enhance Ca 2+ -induced oocyte activation of oocytes matured in vitro.Results Sperm PLC-ζ protein deficiency was demonstrated by Western blot analysis and immunofluorescence and confirmed by reduced PLC-ζ bioactivity using an in vitro model of Ca 2+ release. Nevertheless, with this sperm and supplementation of Ca 2+ ionophore following ICSI, fertilization of four of six oocytes matured in vitro was obtained. In addition, four embryos underwent cleavage and two of them reached the blastocyst stage. Transfer of these blastocysts into the uterus led to a single pregnancy and live birth. Conclusions Deficiency of PLC-ζ in normal-appearing human sperm is associated with impaired Ca 2+ -dependent oocyte activation during ICSI. Under this condition, use of Ca 2+ ionophore following ICSI of oocytes matured in vitro improves embryo developmental competence, possibly through the activation of Ca 2+ -dependent mechanisms governing fertilization and preimplantation embryogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report

Chithiwala

Lee

Hill

et al. 2015

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

show abstract

“…In mouse, inhibiting Ca 2ϩ release at fertilization prevents many activationassociated changes in protein synthesis (Xu et al, 1994). Reciprocally, translation can be stimulated by artificially increasing Ca 2ϩ within the unfertilized egg (Ducibella et al, 2002). A hypothesis for the molecular mechanism of Ca 2ϩ -induced translation in mouse has been inferred from another murine cell type, hippocampal neurons .…”

Section: Maternal Transcript Translationmentioning

confidence: 99%

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

Horner

Wolfner

2008

Developmental Dynamics

183

177

View full text Add to dashboard Cite

The transition from mature oocyte to developing embryo requires a coordinated series of events, collectively known as egg activation. Egg activation includes changes to egg coverings to prevent polyspermy, release of oocyte meiotic arrest, generation of haploid female and male pronuclei, changes in maternal mRNAs and protein populations, and cytoskeletal rearrangements. In many animals, egg activation is triggered by fertilization, which increases intracellular calcium within the oocyte and thereby regulates molecular events of egg activation. In other animals, fertilization-independent external signals, including mechanical stimulation of eggs and/or changes in ionic milieu, trigger activation. Recent studies have clarified the upstream portion of pathways leading to eggshell changes and cell cycle resumption and have identified activation-induced changes in maternal mRNA and protein profiles that can identify molecular players in the downstream events of egg activation. We review signals that trigger activation and how they link to subsequent molecular events of egg activation. Developmental Dynamics 237:527-544, 2008.

show abstract

“…Moreover, the calcium oscillations triggered by the sperm cells function not only in inducing 70 resumption of meiosis but also in many other events [26]. For example, recruitment of specific maternal RNAs [27,28] which is essential for activation of zygotic genome [29] and may be extended to other unknown functions. In addition, using bovine oocytes harvested from ovaries stored for 26-30 h resulted in the great reduction of the blastocyst yield after ICSI [30], though the developmental potential of these oocytes after in vitro fertilizaton (IVF) or somatic cell nuclear 75 transplantation was not impaired [30,31].…”

mentioning

confidence: 99%

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

et al. 2009

View full text Add to dashboard Cite

Regardless of the presence of sperm-borne oocyte-activating factors, activation of bovine oocytes with exogenous activation stimuli is required for further development after intracytoplasmic sperm injection (ICSI). The present study was designed to develop a new activation regimen for improving the blastocyst yield after ICSI of bovine oocytes harvested from ovaries stored at 10-12 oC for 24 h. Following ICSI, oocytes were treated with 5 μM ionomycin for 5 min, 7% ethanol for 5 or 10 min, ionomycin followed by ethanol (5 or 10 min), ionomycin followed by 10 µg/mL Cycloheximide for 5 h, or ionomycin followed by 1.9 mM 6-dimethylaminopurine for 3 h. Across the activation regimens, the cleavage rates of ICSI oocytes (45-77%) were higher than those of parthenogenetically activated oocytes (11-21%; P<0.05). Activating the ICSI oocytes with ionomycin plus ethanol improved the blastocyst yield (29-30%) comparing to the non-treated oocytes (12%; P<0.05) but the other regimens did not (9-18%; P>0.05). The higher blastocyst yields were due to increasing the proportion of ICSI oocytes that passed through the early postfertilization events until cleavage. None of the regimens have any adverse effect on the quality of the blastocysts regarding the total cell number or the proportion of the inner cell mass cells. Thus, a new activation regimen composed from two triggers for single calcium increase has been proven effective to improve the blastocyst yield after bovine ICSI using oocytes harvested from stored ovaries.Dear Dr. John P. Kastelic, Thank you very much for prompt reply and accepting our manuscript for publication in Theriogenology. We have replied for each comment from Co-editor and the reviewer-2 in pointby-point basis.Sincerely yours, Shinichi Hochi (Corresponding author)Hany Abdalla (First author)Co-editor 1-P value has been added to the Abstract (Lines 27,29).2-The number of the references in Introduction section has been reduced. Reviewer #21-Regarding omitting the two groups (5 min ethanol group and ionomycin followed by 5 min ethanol group); We do not think that the presence of these two groups causes any difficulty to recognize the superiority of ionomycin and ethanol combination. Since activation of 7% ethanol for 5 min 4 hr after ICSI is a commonly applied method for bovine ICSI, presence of such group must be important to declare the superiority of the new combination (ionomycin and ethanol) over the methods of ethanol alone under the same experimental circumstance regarding oocyte quality, injection skill, culture condition, and so on. For the ionomycin followed by 5 min ethanol, the efficiency of this method to improve the cleavage and the blastocyst yield was similar to that of ionomycin followed by 10 min ethanol, making it possible to choose the method in which oocytes are exposed to minimum exogenous stimuli. Tables 1 and 2. 2-The time of 2 nd polar body detection has been added in footnote of 15Running head: Bovine oocyte activation after ICSI * Manuscript 2 AbstractRegardless of the pres...

show abstract

Egg-to-Embryo Transition Is Driven by Differential Responses to Ca2+ Oscillation Number

Cited by 362 publications

References 50 publications

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report

Transitioning from egg to embryo: Triggers and mechanisms of egg activation

A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa

Contact Info

Product

Resources

About