Egg Transport in the Rabbit Oviduct: The Roles of Cilia and Muscle

Halbert, Sheridan A.; Tam, P.Y.; Blandau, Richard J.

doi:10.1126/science.1251215

Cited by 185 publications

(112 citation statements)

References 2 publications

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“…Fertility clinics worldwide culture embryos in the static state. Before implantation, the oocytes and zygotes within the oviduct and uterus are constantly moving and being stimulated by the surrounding environment during natural pregnancy [7][8][9]. Moving and stimulating sources that influence embryonic development include shear stress by tubal fluid flow, compression by peristaltic tubal wall movement, buoyancy and kinetic friction forces between the embryo and cilia [10,11].…”

Section: Introductionmentioning

confidence: 99%

Effect of micro-vibration culture system on embryo development

Hur

Park

Ryu

et al. 2013

J Assist Reprod Genet

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Purpose Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders. Materials and methods The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n=178) and a micro-vibration culture group (n=181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n=159 cycles) and a micro-vibration culture group (n=166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.Results The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p<0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p<0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p<0.05). Conclusions Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.

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Section: Introductionmentioning

confidence: 99%

Effect of micro-vibration culture system on embryo development

Hur

Park

Ryu

et al. 2013

J Assist Reprod Genet

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“…After fertilization has occurred in the ampulla of the oviduct, the embryo is transported to the uterus within a few days. The transport to the uterus is caused by waves of contraction and relaxation of the smooth muscle and the ciliation of the oviductal epithelial cells (Halbert et al 1976, Hunter 2012. Prostaglandin (PG) E 2 and PGF 2a concentrations in the oviduct ipsilateral to the corpus luteum (CL) or the dominant follicle are highest in the periovulatory phase (Wijayagunawardane et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Summer heat stress affects prostaglandin synthesis in the bovine oviduct

et al. 2013

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Summer heat stress (HS) negatively affects reproductive functions, including prostaglandin (PG) F 2a secretion in the endometrium, and decreases fertility in cattle. In the present study, we examined the effects of elevated temperatures on PG synthesis in oviductal epithelial cells. The epithelial cells obtained from the ampulla and isthmus of the oviduct were incubated at various temperatures (38.5, 39.5, 40.0, and 40.5 8C) for 24 h. In the ampulla, PGE 2 concentration was higher at 40.5 8C than at 38.5 8C, while PGF 2a production was not affected by the temperatures in this range. The expressions of microsomal PGE synthase 1 (PTGES (mPGES1)), cytosolic PGES (PTGES3 (cPGES)), and heat shock protein 90 (HSP90AA1 (HSP90)) mRNAs and proteins were higher at 40.5 8C than at 38.5 8C in the ampullary epithelial cells. Seasonal changes in the expressions of PGES and HSP90AA1 mRNAs in oviductal tissues were also investigated. The expressions of PTGES3 and HSP90AA1 mRNAs were higher in the ampullary tissues in summer than in winter. In summary, elevated temperatures stimulated PGE 2 production in the ampullary oviduct by increasing the expressions of PGESs and HSP90AA1, which can activate cPGES. The overall results suggest that HS upsets PG secretions and reduces oviductal smooth muscle motility, which in turn could decrease gamete/embryo transport through the oviduct.

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“…Mammalian embryos are transported to the uterine cavity through the fallopian tube during cell cleavage, blastomere, and blastocyst development (Halbert et al 1976, Eddy et al 1980). In conjunction with ciliated epithelium, the fallopian tube acts as a peristaltic pump due to phasic contraction of the smooth muscle in the wall to transport the embryo (Zervomanolakis et al 2007, Lyons et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Improved development of mouse and human embryos using a tilting embryo culture system

Matsuura

Hayashi

Kuroda

et al. 2010

Reproductive BioMedicine Online

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Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression, and friction force, in the fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, we developed the Tilting Embryo Culture System (TECS). The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P<0.05). Although not significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared to static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low quality embryos and those embryos cultured under suboptimal conditions (P<0.05). Here, we propose that the TECS could be a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to in the fallopian tube.

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Egg Transport in the Rabbit Oviduct: The Roles of Cilia and Muscle

Cited by 185 publications

References 2 publications

Effect of micro-vibration culture system on embryo development

Effect of micro-vibration culture system on embryo development

Summer heat stress affects prostaglandin synthesis in the bovine oviduct

Improved development of mouse and human embryos using a tilting embryo culture system

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