Eggplant Germination is Promoted by Hydrogen Peroxide and Temperature in an Independent but Overlapping Manner

Abstract

Hydrogen peroxide promotes seed germination, but the molecular mechanisms underlying this process are unclear. This study presents the results of eggplant (Solanum melongena) germination analyses conducted at two different temperatures and follows the effect of hydrogen peroxide treatment on seed germination and the seed proteome. Hydrogen peroxide was found to promote eggplant germination in a way not dissimilar to that of increased temperature stimuli. LC–MS profiling detected 729 protein families, 77 of which responded to a temperature increase or hydrogen peroxide treatment. These differentially abundant proteins were found to be involved in a number of processes, including protein and amino acid metabolism, carbohydrate metabolism, and the glyoxylate cycle. There was a very low overlap between hydrogen peroxide and temperature-responsive proteins, highlighting the differences behind the seemingly similar outcomes. Furthermore, the observed changes from the seed proteome indicate that hydrogen peroxide treatment diminished the seed endogenous hydrogen peroxide pool and that a part of manifested positive hydrogen peroxide effect might be related to altered sensitivity to abscisic acid.

Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

“…Peptides were eluted for up to a 120 min with a 4-40% acetonitrile gradient. Spectra were acquired at 2 Hz (MS) and 10-20 Hz (MS/MS) using an intensity-dependent mode with a total cycle time of 7 s. The measured spectra were extracted and processed as described previously by Dufková et al [75]. In brief, recalibrated MGF mascot generic format files were searched against the Araport Arabidopsis protein database by Proteome Discoverer 2.1, employing Sequest HT, MS Amanda, and Mascot 2.4 with the following parameters: enzyme-trypsin, max two missed cleavage sites; mass tolerance-35 ppm (MS) and 0.1 Da (MS/MS); modifications-up to three dynamic modifications including Met oxidation, Asn/Gln deamidation, Lys methylation, N-terminal Gln/Glu to pyro-Glu, N-terminal acetylation, and N-terminal Met loss.…”

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

“…Therefore, the observed decrease in most of the amino acid levels at the 48 h time-point following the GABA and VGB treatments might contribute to the inhibition of chestnut seed germination (Figure 6). At 120 h, however, only the VGB treatment induced a considerable increase in the accumulation of 10 amino acids ( Figure 6), which were reportedly negatively associated with root growth [13,37,[58][59][60]. Thus, VGB might have a specific role in inhibiting early primary root growth.…”

“…An exogenous nitric oxide donor (S-nitroso-N-acetyl-d,l-penicillamine) can enhance the germination of Kabuli chickpea seeds, which coincides with an increase in amino acid levels [57]. Exogenous H 2 O 2 also promotes the germination of eggplant seeds, accompanied by enhanced amino acid biosynthesis and protein expression [58]. Therefore, the observed decrease in most of the amino acid levels at the 48 h time-point following the GABA and VGB treatments might contribute to the inhibition of chestnut seed germination (Figure 6).…”

Barley Root Proteome and Metabolome in Response to Cytokinin and Abiotic Stimuli

“…Total protein extracts were prepared as previously described ( Hloušková et al, 2019 ) employing a combination of phenol/acetone/TCA extraction. Portions of samples corresponding to 5 μg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system (Thermo) and a qTOF maXis Impact mass spectrometer (Bruker) as previously described ( Dufková et al, 2019 ). Peptides were eluted with up to a 120-min, 4% to 40% acetonitrile gradient.…”