Egonol gentiobioside and egonol gentiotrioside from Styrax perkinsiae promote the biosynthesis of estrogen by aromatase

Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)

“…The assay was performed as described in a previous study (Lu et al, 2012). KGN cells or UMR-106 cells were seeded in 24-well plates and cultured overnight.…”

“…The qRT-PCR analysis of aromatase expression was conducted as described previously (Lu et al, 2012). Total cellular RNA was isolated using TRIzol reagent according to the manufacturer's instructions (Invitrogen).…”

“…The aromatase protein immunoblotting was conducted as described previously (Lu et al, 2012). KGN cells cultured in 60 mm dishes were lysed with RIPA lysis buffer (Beyotime, Haimen, China) supplemented with a protease inhibitor cocktail (Sigma).…”

Pregnane alkaloids from Sarcococca hookeriana var. digyna

“…The assay for estrogen biosynthesis has been described previously [11]. A 500-μL volume of 5 × 10 5 KGN cells/mL was added to each well of a 24-well tissue culture plate.…”

“…digyna that are responsible for the treatment of rheumatism, phytochemical investigation led to the isolation of three new pregnane-type steroidal alkaloids (1-3) together with eleven known ones (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). The structures of these components were elucidated on the basis of spectral data.…”